scholarly journals The movement proteins of cowpea mosaic virus and cauliflower mosaic virus induce tubular structures in plant and insect cells

1996 ◽  
Vol 77 (11) ◽  
pp. 2857-2864 ◽  
Author(s):  
D. T. J. Kasteel ◽  
M.-C. Perbal ◽  
J.-C. Boyer ◽  
J. Wellink ◽  
R. W. Goldbach ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Insect Cells ◽  
Cauliflower Mosaic Virus ◽  
Cowpea Mosaic Virus ◽  
Tubular Structures ◽  
Movement Proteins

scholarly journals Synthesis of the complete 200K polyprotein encoded by cowpea mosaic virus B-RNA in insect cells

1992 ◽  
Vol 73 (11) ◽  
pp. 2775-2784 ◽  
Author(s):  
H. van Bokhoven ◽  
J. W. M. van Lent ◽  
R. Custers ◽  
J. M. Vlak ◽  
J. Wellink ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Insect Cells ◽  
Cowpea Mosaic Virus

scholarly journals Co-expression of the capsid proteins of Cowpea mosaic virus in insect cells leads to the formation of virus-like particles

2000 ◽  
Vol 81 (12) ◽  
pp. 3093-3097 ◽  
Author(s):  
Michael Shanks ◽  
George P. Lomonossoff
Keyword(s):  
Mosaic Virus ◽  
Spodoptera Frugiperda ◽  
Insect Cells ◽  
Gradient Centrifugation ◽  
Coat Proteins ◽  
Cowpea Mosaic Virus ◽  
Virion Assembly ◽  
Virus Like Particles ◽  
Assembly Pathway ◽  
The Individual

The regions of RNA-2 of Cowpea mosaic virus (CPMV) that encode the Large (L) and Small (S) coat proteins were expressed either individually or together in Spodoptera frugiperda (sf21) cells using baculovirus vectors. Co-expression of the two coat proteins from separate promoters in the same construct resulted in the formation of virus-like particles whose morphology closely resembled that of native CPMV virions. No such particles were formed when the individual L and S proteins were expressed. Sucrose gradient centrifugation of the virus-like particles showed that they had the sedimentation characteristics of empty (protein-only) shells. The results confirm that the 60 kDa L–S fusion is not an obligate intermediate in the virion assembly pathway and indicate that expression of the coat proteins in insect cells will provide a fruitful route for the study of CPMV morphogenesis.

scholarly journals Identification of Inhibitory Mutants of Cauliflower mosaic virus Movement Protein Function after Expression in Insect Cells

1999 ◽  
Vol 73 (9) ◽  
pp. 7886-7890 ◽  
Author(s):  
C. L. Thomas ◽  
A. J. Maule
Keyword(s):  
Mosaic Virus ◽  
Protein Function ◽  
Insect Cells ◽  
Movement Protein ◽  
Dominant Negative ◽  
Cauliflower Mosaic Virus ◽  
Tubule Formation ◽  
Virus Particles ◽  
And Function

ABSTRACT Cauliflower mosaic virus (CaMV) encodes a movement protein (MP) which forms tubules in vivo and mediates the translocation of virus particles through plasmodesmata. The relationship between CaMV MP structure and function, in isolation from the complete virus infection, was studied by using MP expression in insect cells. The study allowed the MP domains necessary for tubule formation to be identified and potential MP-MP interactions to be investigated by using double infections with recombinant baculoviruses. Two MP domains which interfered with the ability of the wild-type MP to form tubules were identified. These mutant domains appeared to act as competitive, rather than dominant negative, inhibitors.

scholarly journals Isolation and characterization of tubular structures of cowpea mosaic virus.

1997 ◽  
Vol 78 (12) ◽  
pp. 3167-3170 ◽  
Author(s):  
D T Kasteel ◽  
J W van Lent ◽  
J Wellink ◽  
R W Goldbach
Keyword(s):  
Mosaic Virus ◽  
Cowpea Mosaic Virus ◽  
Tubular Structures ◽  
Isolation And Characterization

The cowpea mosaic virus M RNA-encoded 48-kilodalton protein is responsible for induction of tubular structures in protoplasts.

1993 ◽  
Vol 67 (6) ◽  
pp. 3660-3664 ◽  
Author(s):  
J Wellink ◽  
J W van Lent ◽  
J Verver ◽  
T Sijen ◽  
R W Goldbach ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Cowpea Mosaic Virus ◽  
Tubular Structures

Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus

2005 ◽  
Vol 112 (1-2) ◽  
pp. 38-41 ◽  
Author(s):  
Takumi Abe ◽  
Norifumi Miyake ◽  
Yasuyuki Nishijima ◽  
Ryousuke Fujita ◽  
Ken Sahara ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Insect Cells ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Cauliflower Mosaic Virus 35S

scholarly journals Tubular structures involved in movement of cowpea mosaic virus are also formed in infected cowpea protoplasts

1991 ◽  
Vol 72 (11) ◽  
pp. 2615-2623 ◽  
Author(s):  
J. van Lent ◽  
M. Storms ◽  
F. van der Meer ◽  
J. Wellink ◽  
R. Goldbach
Keyword(s):  
Mosaic Virus ◽  
Cowpea Mosaic Virus ◽  
Tubular Structures ◽  
Cowpea Protoplasts
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