scholarly journals Halopenitus persicus gen. nov., sp. nov., an archaeon from an inland salt lake

2012 ◽  
Vol 62 (Pt_8) ◽  
pp. 1932-1936 ◽  
Author(s):  
Mohammad Ali Amoozegar ◽  
Ali Makhdoumi-Kakhki ◽  
Seyed Abolhassan Shahzadeh Fazeli ◽  
Reza Azarbaijani ◽  
Antonio Ventosa
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Salt Lake ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family

A novel pale pink-pigmented halophilic archaeon, strain DC30T, was isolated from Aran-Bidgol salt lake, a hypersaline playa in Iran. Cells of strain DC30T were non-motile and pleomorphic, from rods to triangular or disc-shaped. Strain DC30T required at least 1.7 M NaCl and 0.05 M MgCl2 for growth (optimum, 3 M NaCl and 0.1 M MgCl2). The optimum pH and temperature for growth of strain DC30T were pH 7.5 and 40 °C, respectively, although it was capable of growth over pH and temperature ranges of 6.5–8.5 and 25–50 °C, respectively. Analysis of the 16S rRNA gene sequence showed that strain DC30T was a member of the family Halobacteriaceae . However, it had low 16S rRNA gene sequence similarities of 92.4 %, 89.4 % and 89.1 % to the most closely related haloarchaeal taxa, the type species of the genera Halorubrum , Halogranum and Haloplanus , respectively. The DNA G+C content was 66.0 mol%. Phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, common phospholipids found in haloarchaea, were present. Three minor phospholipids and one unidentified glycolipid were also observed. The only quinone present was MK-8(II-H2). The physiological, biochemical and phylogenetic differences between strain DC30T and other previously described genera of extremely halophilic archaea suggest that strain DC30T represents a novel species in a new genus within the family Halobacteriaceae , for which the name Halopenitus persicus gen. nov., sp. nov. is proposed. The type strain of Halopenitus persicus is DC30T ( = IBRC 10041T = KCTC 4046T).

Mangrovimonas yunxiaonensis gen. nov., sp. nov., isolated from mangrove sediment

2013 ◽  
Vol 63 (Pt_6) ◽  
pp. 2043-2048 ◽  
Author(s):  
Yi Li ◽  
Shijie Bai ◽  
Caiyun Yang ◽  
Qiliang Lai ◽  
Huajun Zhang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Mangrove Sediment ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family

A Gram-negative, short-rod-shaped, orange-pigmented bacterium, strain LYYY01T, was isolated from a mangrove sediment sample collected from Yunxiao mangrove National Nature Reserve, Fujian Province, China. 16S rRNA gene sequence comparisons showed that strain LYYY01T is a member of the family Flavobacteriaceae , forming a distinct lineage with species of the genera Meridianimaribacter , Sediminibacter , Gelidibacter and Subsaximicrobium . The 16S rRNA gene sequence similarity between strain LYYY01T and the type strains of related species ranged from 93.9 to 90.9 %. Growth was observed at temperatures from 10 to 38 °C, at salinities from 1 to 7 % and at pH from 6 to 10. The DNA G+C content of the strain was 38.6 mol% and the major respiratory quinone was menaquinone-6 (MK-6). The major fatty acids were iso-C15 : 1 (27.6 %), iso-C15 : 0 (24.0 %), iso-C17 : 0 3-OH (12.0 %) and iso-C16 : 0 3-OH (6.2 %). According to its morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, strain LYYY01T is considered to represent a novel species of a new genus in the family Flavobacteriaceae , for which the name Mangrovimonas yunxiaonensis gen. nov., sp. nov. is proposed. The type strain of Mangrovimonas yunxiaonensis is LYYY01T ( = CGMCC 1.12280T = LMG 27142T).

scholarly journals Salinispirillum marinum gen. nov., sp. nov., a haloalkaliphilic bacterium in the family ‘Saccharospirillaceae’

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3610-3615 ◽  
Author(s):  
Azadeh Shahinpei ◽  
Mohammad Ali Amoozegar ◽  
Seyed Abolhassan Shahzadeh Fazeli ◽  
Peter Schumann ◽  
Antonio Ventosa
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family

A novel Gram-staining-negative, motile, non-pigmented, facultatively anaerobic, spirillum-shaped, halophilic and alkaliphilic bacterium, designated strain GCWy1T, was isolated from water of the coastal–marine wetland Gomishan in Iran. The strain was able to grow at NaCl concentrations of 1–10 % (w/v) and optimal growth was achieved at 3 % (w/v). The optimum pH and temperature for growth were pH 8.5 and 30 °C, while the strain was able to grow at pH 7.5–10 and 4–40 °C. Phylogenetic analysis based on the comparison of the 16S rRNA gene sequence placed the isolate within the class Gammaproteobacteria as a separate deep branch, with 92.1 % or lower sequence similarity to representatives of the genera Saccharospirillum and Reinekea and less than 91.0 % sequence similarity with other remotely related genera. The major cellular fatty acids of the isolate were C18 : 1ω7c, C16 : 0 and C17 : 0, and the major components of its polar lipid profile were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The cells of strain GCWy1T contained the isoprenoid quinones Q-9 and Q-8 (81 % and 2 %, respectively). The G+C content of the genomic DNA of this strain was 52.3 mol%. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic and phenotypic data, strain GCWy1T represents a novel species in a new genus in the family ‘ Saccharospirillaceae ’, order Oceanospirillales , for which the name Salinispirillum marinum gen. nov., sp. nov. is proposed. The type strain of the type species is GCWy1T ( = IBRC-M 10765T = CECT 8342T).

Pontimonas salivibrio gen. nov., sp. nov., a new member of the family Microbacteriaceae isolated from a seawater reservoir of a solar saltern

2013 ◽  
Vol 63 (Pt_6) ◽  
pp. 2124-2131 ◽  
Author(s):  
Gwang Il Jang ◽  
Yirang Cho ◽  
Byung Cheol Cho
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family

A Gram-staining-positive, non-motile, strictly aerobic, non-spore-forming, vibrio-shaped bacterial strain, CL-TW6T, was isolated from a reservoir seawater sample from a solar saltern in Korea. Analysis of the 16S rRNA gene sequence of strain CL-TW6T revealed a clear affiliation with the family Microbacteriaceae . Strain CL-TW6T showed the closest phylogenetic relationships with the genera Yonghaparkia and Microcella , with 16S rRNA gene sequence similarity of 94.8–95.3 %. The strain grew in the presence of 1–9 % sea salts, at 15–35 °C and at pH 7.0–9.0. The major cellular fatty acids of strain CL-TW6T were anteiso-C15 : 0 (32.6 %), iso-C16 : 0 (20.4 %), iso-C15 : 0 (13.2 %) and iso-C14 : 0 (11.8 %) and the major menaquinones were MK-9 and MK-10. Cell-wall analysis showed that the peptidoglycan of strain CL-TW6T contained 2,4-diaminobutyric acid, alanine, glycine and glutamic acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The genomic DNA G+C content of strain CL-TW6T was 60.0 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed clearly that strain CL-TW6T could be distinguished from members of the family Microbacteriaceae with validly published names. Thus, strain CL-TW6T should be classified as representing a novel genus and species in the family Microbacteriaceae , for which the name Pontimonas salivibrio gen. nov., sp. nov. is proposed. The type strain of Pontimonas salivibrio is CL-TW6T ( = KCCM 90105T  = JCM 18206T).

scholarly journals Marininema mesophilum gen. nov., sp. nov., a thermoactinomycete isolated from deep sea sediment, and emended description of the family Thermoactinomycetaceae

2012 ◽  
Vol 62 (Pt_6) ◽  
pp. 1383-1388 ◽  
Author(s):  
Jie Li ◽  
Guang-Tao Zhang ◽  
Jian Yang ◽  
Xin-Peng Tian ◽  
Fa-Zuo Wang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family

A novel filamentous bacterium, strain SCSIO 10219T, was isolated from a sediment sample collected from the South China Sea (113° 3.752′ E 18° 1.722′ N) at a depth of 2105 m. Growth was observed at 25–35 °C (optimum 30 °C) and pH 5.0–8.0 (optimum pH 6.0–7.0). The organism formed yellow–white colonies with radial wrinkles. Aerial mycelium was not produced on any of the growth media tested. Phenotypic characterization and 16S rRNA gene sequence analysis indicated that strain SCSIO 10219T belongs to the family Thermoactinomycetaceae . The strain contained ll-diaminopimelic acid in the cell wall. The predominant menaquinone was MK-7. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and five unknown phospholipids. Major fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The DNA G+C content was 46.5 mol%. On the basis of chemotaxonomic properties and phylogenetic analysis based on 16S rRNA gene sequence data, it is proposed that this strain represents a novel species in a new genus, Marininema mesophilum gen. nov., sp. nov., in the family Thermoactinomycetaceae . The type strain of the type species is SCSIO 10219T ( = CCTCC AA 2011006T = DSM 45610T). In addition, we propose that the description of the family Thermoactinomycetaceae should be further emended based on the present study.

scholarly journals Gracilibacillus kekensis sp. nov., a moderate halophile isolated from Keke Salt Lake

2012 ◽  
Vol 62 (Pt_5) ◽  
pp. 1032-1036 ◽  
Author(s):  
Miao Gao ◽  
Zhan-Zhi Liu ◽  
Yu-Guang Zhou ◽  
Hong-Can Liu ◽  
Yu-Chao Ma ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Salt Lake ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A novel moderately halophilic bacterium, designated strain K170T, was isolated from Keke Salt Lake in Qinghai, China. The strain grew with 0–22 % (w/v) NaCl, at 4–50 °C and at pH 6–11, with optimum growth in 3 % (w/v) NaCl, at 40 °C and at pH 8. The predominant respiratory quinone was menaquinone 7 (MK-7). The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, aminolipids and glycolipids. The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0. The DNA G+C content was 35.8 mol%. Phylogenetic analysis based on the full-length 16S rRNA gene sequence revealed that strain K170T was a member of the genus Gracilibacillus . High levels of 16S rRNA gene sequence similarity were found between strain K170T and Gracilibacillus boraciitolerans DSM 17256T (97.3 %) and Gracilibacillus thailandensis JCM 15569T (97.1 %). 16S rRNA gene sequence similarities between strain K170T and the type strains of other recognized members of the genus Gracilibacillus were below 97 %. The DNA–DNA hybridization values of strain K170T with G. boraciitolerans DSM 17256T and G. thailandensis JCM 15569T were 21.9 % and 34.3 %, respectively. On the basis of these results, strain K170T is considered to represent a novel species of the genus Gracilibacillus , for which the name Gracilibacillus kekensis sp. nov. is proposed; the type strain is K170T ( = CGMCC 1.10681T = DSM 23178T).

scholarly journals Mameliella phaeodactyli sp. nov., a member of the family Rhodobacteraceae isolated from the marine algae Phaeodactylum tricornutum

2015 ◽  
Vol 65 (Pt_5) ◽  
pp. 1617-1621 ◽  
Author(s):  
Zhangran Chen ◽  
Jingyan Zhang ◽  
Xueqian Lei ◽  
Qiliang Lai ◽  
Luxi Yang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phaeodactylum Tricornutum ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family

A novel Gram-staining-negative, aerobic, rod-shaped, non-motile, yellow bacterium designated strain KD53T, was isolated from a culture of the alga Phaeodactylum tricornutum from Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD53T was a member of the Roseobacter clade within the family Rhodobacteraceae , forming a distinct lineage with species of the genus Mameliella . The 16S rRNA gene sequence similarities between strain KD53T and other strains examined were all less than 97.0 %. Strain KD53T was found to grow optimally at 28 °C, at pH 7.5–8.0 and in the presence of 3 % (w/v) NaCl. The dominant fatty acids of strain KD53T were C18 : 1ω6c and/or C18 : 1ω7c, C18 : 0 and C16 : 0. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 65 mol% and the major respiratory quinone was ubiquinone 10 (Q-10). On the basis of phenotypic data and phylogenetic inference, strain KD53T represents a novel member of the genus Mameliella , then the name Mameliella phaeodactyli sp. nov. is proposed. The type strain is KD53T ( = MCCC 1K00273T = KCTC 42178T).

scholarly journals Aidingimonas lacisalsi sp. nov., isolated from a salt lake in China

2020 ◽  
Vol 70 (3) ◽  
pp. 1678-1683 ◽  
Author(s):  
Tong-Wei Guan ◽  
Li-Sha Tian ◽  
Shu-Kun Tang ◽  
Lei Tian ◽  
Yu-Zhou Feng
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Gene Sequence ◽  
Salt Lake ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A novel bacterium, XHU 5135T, belonging to the genus Aidingimonas , was isolated from a salt lake sample collected in Xinjiang Province, north-west PR China. The isolate was Gram-stain-negative, rod-shaped and non-motile. The strain was catalase-positive and oxidase-negative. Growth occurred at NaCl concentrations of 5–25 % (optimum, 10–13 %), at 13–41 °C (35–37 °C) and at pH 6.0–10.0 (pH 7.0–8.0). The predominant ubiquinone was Q-9. The major fatty acids were C19 : 0 cyclo ω8c and C16 : 0. The G+C content of the genomic DNA was 58.1 mol%. The affiliation of strain XHU 5135T with the genus Aidingimonas was confirmed by 16S rRNA gene sequence comparisons. The closest type strain was Aidingimonas halophile YIM 90637T, which showed a 16S rRNA gene sequence similarity of 97.5 %. The ANI value between XHU 5135T and the closest type strain was 80.01 %. The estimated digital DNA–DNA hybridization estimate value between strain XHU 5135T and the closest type strain was 22.80 %. Phenotypically, the characteristics of XHU 5135T were shown to differ from the most closely related species, A. halophila . On the basis of the data from this polyphasic study, strain XHU 5135T represents a novel species of the genus Aidingimonas , for which the name Aidingimonas lacisalsi sp. nov. is proposed. The type strain is strain XHU 5135T (=CCTCC AB 2016344T=KCTC 42945T=DSM 104700T).

scholarly journals Sinomicrobium pectinilyticum sp. nov., a pectinase-producing bacterium isolated from alkaline and saline soil, and emended description of the genus Sinomicrobium

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 2939-2943 ◽  
Author(s):  
Bin Cheng ◽  
Chunfang Li ◽  
Qiliang Lai ◽  
Miaofen Du ◽  
Zongze Shao ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family

A Gram-reaction-negative, non-spore-forming strain, designated 5DNS001T, was isolated from soil of an ancient salt-extracting facility in China. Analysis of the almost-complete 16S rRNA gene sequence of the bacterium suggested that it belongs to the genus Sinomicrobium in the family Flavobacteriaceae . It exhibited highest 16S rRNA gene sequence similarity with Sinomicrobium oceani SCSIO 03483T (96.3 %), but less than 93 % sequence similarity with members of the genera Imtechella , Zhouia and Joostella and other recognized members of the family Flavobacteriaceae . The strain was able to hydrolyse pectin and starch by producing pectinase and α-amylase. The DNA G+C content of the strain was 42.6 mol%. The major respiratory quinone was MK-6. The major polar lipid detected in the strain was phosphatidylethanolamine. The dominant cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). Based on phenotypic, genotypic, chemotaxonomic and phylogenetic analyses, a novel species, Sinomicrobium pectinilyticum, is proposed. The type strain is 5DNS001T ( = CGMCC1.11000T = KCTC23776T).

scholarly journals Phaeodactylibacter xiamenensis gen. nov., sp. nov., a member of the family Saprospiraceae isolated from the marine alga Phaeodactylum tricornutum

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3496-3502 ◽  
Author(s):  
Zhangran Chen ◽  
Xueqian Lei ◽  
Qiliang Lai ◽  
Yi Li ◽  
Bangzhou Zhang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phaeodactylum Tricornutum ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
The Family

A novel Gram-staining-negative, aerobic, rod-shaped, non-motile, reddish-orange and chemoheterotrophic bacteria, designated strain KD52T, was isolated from a culture of the alga Phaeodactylum tricornutum from Xiamen, Fujian Province, China. 16S rRNA gene sequence comparison showed that strain KD52T was a member of the family Saprospiraceae , forming a distinct lineage with ‘Portibacter lacus’ KCTC 23747. The 16S rRNA gene sequence similarity between strain KD52T and the type strains of species of the family Saprospiraceae ranged from 86 % to 89 %. Growth occurred at 20–37 °C (optimum, 28 °C), in the presence of 1–9 % (w/v) NaCl (optimum, 2.5 %) and at pH 5–8.5 (optimum, pH 6.0). The dominant fatty acids (>10 %) of strain KD52T were iso-C15 : 0 (33.1 %), iso-C15 : 1 G (14.8 %) and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c, 13.8 %). The major polar lipids were diphosphatidylglycerol, three unidentified phospholipids, four unknown lipids and one unidentified aminolipid. The DNA G+C content was 51 mol% and the major respiratory quinone was menaquinone-7 (MK-7). On the basis of phenotypic data and phylogenetic inference, strain KD52T represents a novel species of a new genus, for which the name Phaeodactylibacter xiamenensis gen. nov., sp. nov., is proposed. The type strain is KD52T ( = MCCC 1F01213T = KCTC 32575T).

Salinibacter iranicus sp. nov. and Salinibacter luteus sp. nov., isolated from a salt lake, and emended descriptions of the genus Salinibacter and of Salinibacter ruber

2012 ◽  
Vol 62 (Pt_7) ◽  
pp. 1521-1527 ◽  
Author(s):  
Ali Makhdoumi-Kakhki ◽  
Mohammad Ali Amoozegar ◽  
Antonio Ventosa
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Salt Lake ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Two Gram-staining-negative, red- and orange-pigmented, non-motile, rod-shaped, extremely halophilic bacteria, designated strains CB7T and DGOT, were isolated from Aran-Bidgol salt lake, Iran. Growth occurred at NaCl concentrations of between 2 and 5 M NaCl and the isolates grew optimally with 3 M NaCl. The optimum pH and temperature for growth of the two strains were pH 7.5 and 37 °C, and they were able to grow over pH and temperature ranges of pH 6–8 and 25–50 °C. The predominant fatty acids of the two isolates were C18 : 1ω7c, iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The polar lipid pattern of the two isolates consisted of diphosphatidylglycerol, phosphatidylcholine, three unidentified lipids, one unidentified aminolipid and three unidentified glycolipids. The only quinone present was menaquinone 7 (MK-7). The G+C contents of the genomic DNA of strains CB7T and DGOT were 64.8 and 65.6 mol%, respectively. 16S rRNA gene sequence analysis indicated that strains CB7T and DGOT were related to Salinibacter ruber in the phylum Bacteroidetes . Levels of 16S rRNA gene sequence similarity between strains CB7T and DGOT and Salinibacter ruber DSM 13855T were 93.2 and 93.6 %, respectively. The two novel strains shared 98.9 % 16S rRNA gene sequence similarity. DNA–DNA hybridization experiments between strains CB7T and DGOT and Salinibacter ruber DSM 13855T indicated levels of relatedness of 44 and 52 %, respectively, while the level of relatedness between the two new isolates was 53 %. Chemotaxonomic data supported the placement of strains CB7T and DGOT in the genus Salinibacter . DNA–DNA hybridization studies and biochemical and physiological characterization allowed strains CB7T and DGOT to be differentiated from Salinibacter ruber and from each other. They are therefore considered to represent two novel species of the genus Salinibacter , for which the names Salinibacter iranicus sp. nov. (type strain CB7T = IBRC-M 10036T = CGMCC 1.11003T) and Salinibacter luteus sp. nov. (type strain DGOT = IBRC-M 10423T = CGMCC 1.11002T) are proposed. Emended descriptions of the genus Salinibacter and of Salinibacter ruber are also presented.

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