Sphingobacterium thermophilum sp. nov., of the phylum Bacteroidetes , isolated from compost

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1584-1588 ◽  
Author(s):  
Shuhei Yabe ◽  
Yoshifumi Aiba ◽  
Yasuteru Sakai ◽  
Masaru Hazaka ◽  
Kazuyoshi Kawahara ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A Gram-negative bacterium, designated CKTN2T, was isolated from compost. Cells of strain CKTN2T were strictly aerobic rods. The isolate grew at 20–50 °C (optimum 40–45 °C), but not below 15 °C or above 52 °C, and at pH 5.9–8.8 (optimum pH 7.0), but not below pH 5.4 or above pH 9.3. The DNA G+C content was 40.3 mol%. The predominant menaquinone was MK-7. The major fatty acids were iso-C15 : 0 (45.2 %), iso-C17 : 0 3-OH (11.1 %) and C18 : 0 (14.5 %). Analysis of the 16S rRNA gene sequence of strain CKTN2T revealed that it is a member of the genus Sphingobacterium and is most closely related to Sphingobacterium alimentarium DSM 22362T (93.2 % 16S rRNA gene sequence similarity). Strain CKTN2T could be distinguished from its closest phylogenetic relatives by different phenotypic characteristics. According to the phenotypic and genotypic characteristics, strain CKTN2T represents a novel species of the genus Sphingobacterium , for which the name Sphingobacterium thermophilum sp. nov. is proposed. The type strain is CKTN2T ( = JCM 17858T  = KCTC 23708T).

scholarly journals Faecalicoccus acidiformans gen. nov., sp. nov., isolated from the chicken caecum, and reclassification of Streptococcus pleomorphus (Barnes et al. 1977), Eubacterium biforme (Eggerth 1935) and Eubacterium cylindroides (Cato et al. 1974) as Faecalicoccus pleomorphus comb. nov., Holdemanella biformis gen. nov., comb. nov. and Faecalitalea cylindroides gen. nov., comb. nov., respectively, within the family Erysipelotrichaceae

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3877-3884 ◽  
Author(s):  
Celine De Maesschalck ◽  
Filip Van Immerseel ◽  
Venessa Eeckhaut ◽  
Siegrid De Baere ◽  
Margo Cnockaert ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Strains LMG 27428T and LMG 27427 were isolated from the caecal content of a chicken and produced butyric, lactic and formic acids as major metabolic end products. The genomic DNA G+C contents of strains LMG 27428T and LMG 27427 were 40.4 and 38.8 mol%. On the basis of 16S rRNA gene sequence similarity, both strains were most closely related to the generically misclassified Streptococcus pleomorphus ATCC 29734T. Strain LMG 27428T could be distinguished from S. pleomorphus ATCC 29734T based on production of more lactic acid and less formic acid in M2GSC medium, a higher DNA G+C content and the absence of activities of acid phosphatase and leucine, arginine, leucyl glycine, pyroglutamic acid, glycine and histidine arylamidases, while strain LMG 27428 was biochemically indistinguishable from S. pleomorphus ATCC 29734T. The novel genus Faecalicoccus gen. nov. within the family Erysipelotrichaceae is proposed to accommodate strains LMG 27428T and LMG 27427. Strain LMG 27428T ( = DSM 26963T) is the type strain of Faecalicoccus acidiformans sp. nov., and strain LMG 27427 ( = DSM 26962) is a strain of Faecalicoccus pleomorphus comb. nov. (type strain LMG 17756T = ATCC 29734T = DSM 20574T). Furthermore, the nearest phylogenetic neighbours of the genus Faecalicoccus are the generically misclassified Eubacterium cylindroides DSM 3983T (94.4 % 16S rRNA gene sequence similarity to strain LMG 27428T) and Eubacterium biforme DSM 3989T (92.7 % 16S rRNA gene sequence similarity to strain LMG 27428T). We present genotypic and phenotypic data that allow the differentiation of each of these taxa and propose to reclassify these generically misnamed species of the genus Eubacterium formally as Faecalitalea cylindroides gen. nov., comb. nov. and Holdemanella biformis gen. nov., comb. nov., respectively. The type strain of Faecalitalea cylindroides is DSM 3983T = ATCC 27803T = JCM 10261T and that of Holdemanella biformis is DSM 3989T = ATCC 27806T = CCUG 28091T.

scholarly journals Oceanobacillus luteolus sp. nov., isolated from soil

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1495-1500 ◽  
Author(s):  
Min Wu ◽  
Guiqin Yang ◽  
Zhen Yu ◽  
Li Zhuang ◽  
Yingqiang Jin ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Two Gram-stain-positive, rod-shaped and endospore-forming bacteria, designated WM-1T and WM-4, were isolated from a paddy soil and a forest soil, respectively, in South China. Comparative 16S rRNA gene sequence analyses showed that both strains were members of the genus Oceanobacillus and most closely related to Oceanobacillus chironomi LMG 23627T with pairwise sequence similarity of 96.0 %. The isolates contained menaquinone-7 (MK-7) as the respiratory quinone and anteiso-C15 : 0, anteiso-C17 : 0 and iso-C15 : 0 as the major fatty acids (>10 %). Polar lipids consisted of a predominance of diphosphatidylglycerol and moderate to minor amounts of phosphatidylglycerol and phosphatidylinositol. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The DNA G+C content was 38.6–39.2 mol%. The 16S rRNA gene sequence of strain WM-1T displayed 99.7 % similarity to that of strain WM-4, and DNA–DNA hybridization between the two strains showed a relatedness value of 91 %. Based on the results of this polyphasic study, strains WM-1T and WM-4 represent a novel species in the genus Oceanobacillus , for which the name Oceanobacillus luteolus sp. nov. is proposed. The type strain is WM-1T ( = KCTC 33119T = CGMCC 1.12406T).

scholarly journals Cellulomonas rhizosphaerae sp. nov., a novel actinomycete isolated from soil

2019 ◽  
Vol 69 (4) ◽  
pp. 1001-1008 ◽  
Author(s):  
Yuanyuan Tian ◽  
Chuanyu Han ◽  
Jiangmeihui Hu ◽  
Junwei Zhao ◽  
Chen Zhang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A novel actinomycete, designated strain NEAU-TCZ24T, was isolated from soil and characterized using a polyphasic approach. The results of phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Cellulomonas and formed a stable clade with its closest relatives Cellulomonas terrae JCM 14899T (98.4 % 16S rRNA gene sequence similarity), Cellulomonas xylanilytica JCM 14281T (97.9 %) and Cellulomonas humilata JCM 11945T (97.7 %). The major menaquinones were identified as MK-9(H4) and MK-8(H4). The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositolmannoside, a ninhydrin-positiveglycolipid, an unidentified phosphoglycolipid, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as anteiso-C15 : 0, C18 : 1ω9c, C16 : 0 and anteiso-C17 : 0. Moreover, morphological and chemotaxonomic properties of NEAU-TCZ24T also confirmed the affiliation of the isolate to the genus Cellulomonas . However, multilocus sequence analysis based on five other house-keeping genes (gyrB, rpoB, recA, relA and atpD), DNA–DNA relatedness, physiological and biochemical data indicated that NEAU-TCZ24T could be distinguished from its closest relatives. Therefore, it is proposed that NEAU-TCZ24T represents a novel species of the genus Cellulomonas , for which the name Cellulomonas rhizosphaerae sp. nov. is proposed. The type strain is NEAU-TCZ24T (=CCTCC AA 2018042T=JCM 32383T).

scholarly journals Sphingomonas starnbergensis sp. nov., isolated from a prealpine freshwater lake

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 1017-1023 ◽  
Author(s):  
Hong Chen ◽  
Mareike Jogler ◽  
Brian J. Tindall ◽  
Hans-Peter Klenk ◽  
Manfred Rohde ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A novel type of freshwater bacterium was isolated from the prealpine mesotrophic Starnberger See (Bavaria, southern Germany). Cells of strain 382T were Gram-negative and rod-shaped, motile and creamy-white. The isolate was strictly aerobic, catalase- and oxidase-positive, and grew at pH values of 6–9 (optimum, pH 7) and temperatures of 10–37 °C (optimum, 28 °C). The genomic G+C content of strain 382T was 64.1 mol%. Based on 16S rRNA gene sequence analyses, strain 382T belongs to the family Sphingomonadaceae and clusters within the genus Sphingomonas . Sphingomonas histidinilytica UM 2T and Sphingomonas wittichii DSM 6014T were the closest relatives, as indicated by the highest 16S rRNA gene sequence similarities (97.1 % and 96.8 %, respectively). Sphingomonas paucimobilis DSM 1098T (the type species of the genus Sphingomonas ) exhibited 95.3 % sequence similarity. This affiliation of strain 382T to the genus Sphingomonas is confirmed by the presence of Q-10 as the major respiratory quinone, two sphingoglycolipids, C14 : 0 2-OH as the major 2-hydroxy fatty acid and sym-homospermidine as the major polyamine. The main cellular fatty acids of strain 382T were C18 : 1ω7c (39 %), C16 : 1ω7c (21 %), C16 : 0 (10 %) and C14 : 0 2-OH (10 %). Based on the phylogenetic distance from other species of the genus Sphingomonas and its unusually high C16 : 1ω7c content, strain 382T represents a novel species of the genus Sphingomonas , for which the name Sphingomonas starnbergensis is proposed. The type strain is 382T ( = DSM 25077T  = LMG 26763T).

Phocaeicola faecicola sp. nov., isolated from porcine faeces

2021 ◽  
Vol 71 (9) ◽  
Author(s):  
Ji Young Choi ◽  
Seung-Hyeon Choi ◽  
Jam-Eon Park ◽  
Ji-Sun Kim ◽  
Jiyoung Lee ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

An obligately anaerobic, non-motile, Gram-negative and rod-shaped strain (AGMB03916T) was isolated from faeces of a 2-week-old piglet raised at the National Institute of Animal Science in Wanju, Republic of Korea. Growth of strain AGMB03916T occurred at 30–45 °C (optimum, 37 °C), at pH 6–9 (optimum, pH 8) and in the presence of 0.5–1.0 % (w/v) NaCl. Based on the results of 16S rRNA gene sequence analyses, strain AGMB03916T was closely related to two validly published species of the genus Phocaeicola , Phocaeicola plebeius and Phocaeicola coprocola . The 16S rRNA gene sequence similarity of strain AGMB03916T compared to P. plebeius M12T (=KCTC 5793T) and P. coprocola M16T (=KCTC 5443T) were 96.3 and 95.0 %, respectively. The genomic DNA G+C content of strain AGMB03916T was 46.4 mol%. The average nucleotide identity values between strain AGMB03916T and the reference strains were 74.9–78.5 %. Cells were able to utilize d-glucose, lactose, sucrose, maltose, salicin, aesculin hydrolysis, cellobiose and raffinose. The major end product of metabolism was acetate. The major cellular fatty acids (>10 %) of the isolate were iso-C15 : 0, anteiso-C15 : 0, C16 : 0, C16 : 0 3-OH and summed feature 11 (iso-C17 : 0 3-OH and/or C18 : 2 DMA). On the basis of the genotypic, biochemical, chemotaxonomic, phenotypic and phylogenetic data, strain AGMB03916T represents a novel species of the genus Phocaeicola , for which the name Phocaeicola faecicola sp. nov. is proposed. The type strain is AGMB03916T (=KCTC 25014T=GDMCC 1.2574T).

scholarly journals Pseudoalteromonas arabiensis sp. nov., a marine polysaccharide-producing bacterium

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1805-1809 ◽  
Author(s):  
Hidetoshi Matsuyama ◽  
Hideki Minami ◽  
Hirokazu Kasahara ◽  
Yoshihisa Kato ◽  
Masafumi Murayama ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A novel exopolysaccharide-producing bacterium, designated strain k53T, was isolated from sediment from the Arabia Sea, Indian Ocean. The strain was Gram-negative, motile, strictly aerobic, oxidase-positive and catalase-positive, and required Na+ for growth. Its major isoprenoid quinone was ubiquinone-8 (Q-8), and its cellular fatty acid profile mainly consisted of C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. The DNA G+C content was 43 mol%. 16S rRNA gene sequence analysis suggested that strain k53T is a member of the genus Pseudoalteromonas . Strain k53T exhibited close phylogenetic affinity to Pseudoalteromonas lipolytica LMEB 39T (98.0% 16S rRNA gene sequence similarity) and Pseudoalteromonas donghaensis HJ51T (97.3 %).The DNA–DNA reassociation values between strain k53T and P. lipolytica JCM 15903T and P. donghaensis LMG 24469T were 17 % and 12 %, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, and phylogenetic analysis based on the 16S rRNA gene sequence and DNA–DNA relatedness data, the isolate merits classification as a representative of a novel species, for which the name Pseudoalteromonas arabiensis is proposed. The type strain of this species is k53T ( = JCM 17292T = NCIMB 14688T).

Chryseobacterium gwangjuense sp. nov., isolated from soil

2013 ◽  
Vol 63 (Pt_12) ◽  
pp. 4580-4585 ◽  
Author(s):  
Yong Jin Park ◽  
Heung-Min Son ◽  
Eun-Hee Lee ◽  
Ju Han Kim ◽  
Gafurjon T. Mavlonov ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterial strain, THG-A18T, was isolated from soil of Gwangju province in South Korea. Strain THG-A18T grew optimally at 25–30 °C, at pH 7.0–8.0 and in the absence of NaCl. Strain THG-A18T displayed β-glucosidase activity, which enabled it to convert ginsenoside Rb1 to Rd. According to 16S rRNA gene sequence analysis, strain THG-A18T was shown to belong to the genus Chryseobacterium . The closest phylogenetic neighbours were Chryseobacterium ginsenosidimutans THG 15T (97.9 % 16S rRNA gene sequence similariity), C. defluvii B2T (97.7 %), C. daeguense K105T (97.6 %), C. taiwanense BCRC 17412T (97.5 %), C. indoltheticum LMG 4025T (97.4 %), C. gregarium P 461/12T (97.4 %) and C. lathyri RBA2-6T (97.3 %), but DNA–DNA relatedness values between these strains and strain THG-A18T were below 41.9 %. The G+C content of the genomic DNA was 36.4 mol%. The major respiratory quinone (MK-6) and fatty acids [iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (comprising iso-C17 : 1ω9c and/or 10-methyl C16 : 0)] supported the affiliation of strain THG-A18T with the genus Chryseobacterium . The polar lipids of strain THG-A18T were phosphatidylethanolamine, four unidentified aminolipids and seven unidentified lipids. A number of physiological and biochemical tests allowed phenotypic differentiation of strain THG-A18T from recognized species of the genus Chryseobacterium . The name Chryseobacterium gwangjuense sp. nov. is proposed, with THG-A18T ( = KACC 16227T = LMG 26579T) as the type strain.

Gracilimonas rosea sp. nov., isolated from tropical seawater, and emended description of the genus Gracilimonas

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4006-4011 ◽  
Author(s):  
Yirang Cho ◽  
Hyunwoo Chung ◽  
Gwang Il Jang ◽  
Dong Han Choi ◽  
Jae Hoon Noh ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A Gram-staining-negative, non-motile, spore-forming, rod-shaped, marine bacterial strain, CL-KR2T, was isolated from tropical seawater near Kosrae, an island in the Federated States of Micronesia. Analysis of the 16S rRNA gene sequence of strain CL-KR2T revealed a clear affiliation with the genus Gracilimonas . Based on phylogenetic analysis, strain CL-KR2T showed the closest phylogenetic relationship to Gracilimonas tropica CL-CB462T, with 16S rRNA gene sequence similarity of 96.6 %. DNA–DNA relatedness between strain CL-KR2T and G. tropica CL-CB462T was 6.7 % (reciprocal 9.5 %). Strain CL-KR2T grew in the presence of 1–20 % sea salts and the optimal salt concentration was 3.5–5 %. The temperature and pH optima for growth were 35 °C and pH 7.5. The major cellular fatty acids (≥10.0 %) of strain CL-KR2T were iso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) and iso-C17 : 1ω9c and the only isoprenoid quinone was MK-7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid, two unidentified glycolipids and two unidentified lipids. The genomic DNA G+C content of strain CL-KR2T was 43.2 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain CL-KR2T could be distinguished from the only member of the genus Gracilimonas with a validly published name. Thus, strain CL-KR2T should be assigned to a novel species in the genus Gracilimonas , for which the name Gracilimonas rosea sp. nov. is proposed. The type strain is CL-KR2T ( = KCCM 90206T = JCM 18898T).

Nonomuraea jabiensis sp. nov., isolated from arid soil

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 212-218 ◽  
Author(s):  
Mustafa Camas ◽  
Anil Sazak ◽  
Cathrin Spröer ◽  
Hans-Peter Klenk ◽  
Demet Cetin ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

A novel actinomycete, strain A4036T, was isolated from a soil sample collected from the Jabi district in Abuja, Nigeria. The taxonomic position of strain A4036T was established using a combination of genotypic and phenotypic analyses. The organism formed extensively branched substrate and aerial hyphae that generated spiral chains of spores with warty surfaces. The cell wall contained meso-diaminopimelic acid and the cell-wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinone was MK-9(H4). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylinositol mannoside, hydroxy-phosphatidylethanolamine, hydroxy-phosphatidylmethylethanolamine, two unidentified phospholipids and four unknown glucosamine-containing phospholipids. The major cellular fatty acids were iso-C16 : 0 2-OH, iso-C16 : 0 and 10-methyl C17 : 0. On the basis of 16S rRNA gene sequence similarity studies, strain A4036T grouped in the genus Nonomuraea , being most closely related to Nonomuraea angiospora IFO 13155T (99.05 %), Nonomuraea candida HMC10T (98.78 %), Nonomuraea kuesteri GW 14-1925T (98.49 %), Nonomuraea endophytica YIM 65601T (98.42 %), Nonomuraea maheshkhaliensis 16-5-14T (98.40 %), Nonomuraea turkmeniaca DSM 43926T (98.38 %), Nonomuraea helvata IFO 14681T (98.29 %), Nonomuraea rubra DSM 43768T (98.10 %) and Nonomuraea salmonea DSM 43678T (98.06 %). Levels of 16S rRNA gene sequence similarity to the type strains of other species of the genus Nonomuraea were <98 %. Despite the high 16S rRNA gene sequence similarities, DNA–DNA relatedness values and phenotypic data demonstrated that strain A4036T was clearly distinguished from all closely related species of the genus Nonomuraea . Thus, this isolate is considered to represent a novel species of the genus Nonomuraea , for which the name Nonomuraea jabiensis sp. nov. is proposed. The type strain is A4036T ( = DSM 45507T = KCTC 19870T).

scholarly journals Bacillus oleivorans sp. nov., a diesel oil-degrading and solvent-tolerant bacterium

2015 ◽  
Vol 65 (Pt_4) ◽  
pp. 1310-1315 ◽  
Author(s):  
M. Azmatunnisa ◽  
K. Rahul ◽  
Y. Subhash ◽  
Ch. Sasikala ◽  
Ch. V. Ramana
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Diesel Oil ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type

Two Gram-stain-positive, diesel oil-degrading, solvent-tolerant, aerobic, endospore-forming, rod-shaped bacteria were isolated from a contaminated laboratory plate. Based on 16S rRNA gene sequence analysis, strains JC228T and JC279 were identified as belonging to the genus Bacillus within the family Bacillaceae of the phylum Firmicutes and were found to be most closely related to Bacillus carboniphilus JCM 9731T (98.1 % 16S rRNA gene sequence similarity) and shared <96.0 % 16S rRNA gene sequence similarity with other members of the genus Bacillus . The DNA–DNA hybridization value between the two strains was 88±2 %. Strain JC228T showed 23.4±1 % reassociation (based on DNA–DNA hybridization) with B. carboniphilus LMG 18001T. The DNA G+C content of strains JC228T and JC279 was 39 and 38.4 mol%, respectively. Both strains were positive for catalase and oxidase activities, and negative for hydrolysis of starch and Tween 80. Strains JC228T and JC279 grew chemoorganoheterotrophically with optimum growth at pH 7 (range pH 7–9.5) and 35 °C (range 25–40 °C). Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid (PL2) were the major polar lipids. Major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C17 : 0 and C16 : 0. Whole-cell hydrolysates contained l-alanine, d-alanine, d-glutamic acid and meso-diaminopimelic acid. Both strains utilized diesel oil as sole carbon and energy source. The results of physiological, biochemical, chemotaxonomic and molecular analyses allowed clear differentiation of strains JC228T and JC279 from their closest phylogenetic neighbours. Therefore strains JC228T and JC279 represent a novel species of the genus Bacillus , for which the name Bacillus oleivorans sp. nov. is proposed. The type strain is JC228T ( = LMG 28084T = CCTCC AB 2013353T).

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