Reconstruction of C3 and C4 metabolism in Methylobacterium extorquens AM1 using transposon mutagenesis

Microbiology ◽  
2003 ◽  
Vol 149 (3) ◽  
pp. 601-609 ◽  
Author(s):  
Stephen J. Van Dien ◽  
Yoko Okubo ◽  
Melinda T. Hough ◽  
Natalia Korotkova ◽  
Tricia Taitano ◽  
...  
Keyword(s):  
Transposon Mutagenesis ◽  
Methylobacterium Extorquens ◽  
Methylobacterium Extorquens Am1 ◽  
C4 Metabolism

scholarly journals Novel Methylotrophy Genes of Methylobacterium extorquens AM1 Identified by using Transposon Mutagenesis Including a Putative Dihydromethanopterin Reductase

2003 ◽  
Vol 185 (2) ◽  
pp. 669-673 ◽  
Author(s):  
Christopher J. Marx ◽  
Brooke N. O'Brien ◽  
Jennifer Breezee ◽  
Mary E. Lidstrom
Keyword(s):  
Dihydrofolate Reductase ◽  
Transposon Mutagenesis ◽  
Methylobacterium Extorquens ◽  
Mutagenesis Screen ◽  
Methylobacterium Extorquens Am1 ◽  
Facultative Methylotroph ◽  
Formaldehyde Oxidation

ABSTRACT Ten novel methylotrophy genes of the facultative methylotroph Methylobacterium extorquens AM1 were identified from a transposon mutagenesis screen. One of these genes encodes a product having identity with dihydrofolate reductase (DHFR). This mutant has a C1-defective and methanol-sensitive phenotype that has previously only been observed for strains defective in tetrahydromethanopterin (H4MPT)-dependent formaldehyde oxidation. These results suggest that this gene, dmrA, may encode dihydromethanopterin reductase, an activity analogous to that of DHFR that is required for the final step of H4MPT biosynthesis.

scholarly journals Structure of Methylene-Tetrahydromethanopterin Dehydrogenase from Methylobacterium extorquens AM1

Structure ◽  
2002 ◽  
Vol 10 (8) ◽  
pp. 1127-1137 ◽  
Author(s):  
Ulrich Ermler ◽  
Christoph H. Hagemeier ◽  
Annette Roth ◽  
Ulrike Demmer ◽  
Wolfgang Grabarse ◽  
...  
Keyword(s):  
Methylobacterium Extorquens ◽  
Methylobacterium Extorquens Am1
Sign in / Sign up

Export Citation Format

Share Document