scholarly journals Modeling of Deformable Cell Separation in a Microchannel with Sequenced Pillars

2021 ◽  
Author(s):  
Scott J. Hymel ◽  
Hideki Fujioka ◽  
Damir B. Khismatullin
Keyword(s):  
Cell Migration ◽  
Numerical Analysis ◽  
Shear Flow ◽  
Cell Separation ◽  
Separation Efficiency ◽  
Lattice Line ◽  
Rigid Particles ◽  
Circulating Cells ◽  
Cell Elasticity ◽  
Size Based Separation

AbstractEmbedded pillar microstructures are an efficient approach for controlling and sculpting shear flow in a microchannel but have not yet demonstrated to be effective for deformability-based cell separation and sorting. Although simple pillar configurations (lattice, line sequence) worked well for size-based separation of rigid particles, they had a low separation efficiency for circulating cells. The objective of this study was to optimize sequenced microstructures for separation of deformable cells. This was achieved by numerical analysis of pairwise cell migration in a microchannel with multiple pillars, which size, longitudinal spacing, and lateral location as well as the cell elasticity and size varied. This study revealed two basic pillar configurations optimized for deformability-based separation: 1) “duplet” that consists of two closely spaced pillars positioned far below the centerline and above the centerline halfway to the wall; and 2) “triplet” composed of three widely-spaced pillars located below, above and at the centerline, respectively. The duplet configuration is well suited for deformable cell separation in short channels, while the triplet or a combination of duplets and triplets provides even better separation in long channels. These optimized pillar microstructures can dramatically improve microfluidic methods for sorting and isolation of blood and rare circulating tumor cells.

An evaluation of cell separation techniques in a model mixed cell population

1992 ◽  
Vol 102 (4) ◽  
pp. 789-798
Author(s):  
S.J. Murphy ◽  
D.J. Watt ◽  
G.E. Jones
Keyword(s):  
Cell Population ◽  
Cell Separation ◽  
Separation Efficiency ◽  
Neuronal Cell ◽  
Quantitative Measure ◽  
Precursor Cells ◽  
Muscle Fibres ◽  
Myogenic Cells ◽  
Muscle Precursor ◽  
Muscle Precursor Cells

Muscle precursor cells may act not only as a means of inserting normal genes into diseased muscle fibres, in order to correct or alleviate a genetically inherited myopathy, but recent demonstrations have shown they may prove an invaluable tool for the expression of, and systemic dissemination of, non-muscle gene products. If muscle precursor cells are proved to act as such widespread vectors in terms of gene therapy, then it is imperative that methods are properly elucidated to produce large populations of pure viable myogenic cells for such purposes. In the past, many methods of cell separation have been investigated but carry with them the problems of either a lack of myogenic purity of the population or poor percentage recovery of the original cell population. In the present work we have investigated two methods for segregating myogenic from non-myogenic cells and have critically reviewed the efficiency of separation of the two techniques used. To obtain a quantitative measure of separation efficiency, segregation was carried out on a 1:1 mixture of murine C2 myogenic and murine 3T3 fibroblastic cells. To distinguish between C2 and 3T3 cells, the latter were prelabelled with the fluorescent strain carboxyfluorescein diacetate succinimyl ester (CFSE). Once incorporated into the cell, CFSE remains there, thus preventing transfer of the label to C2 cells. Both methods of separation used depend on the affinity of myogenic cells for the monoclonal antibody Mab H28, which specifically binds to the mouse neuronal cell adhesion molecule N-CAM, but differ in that one method, “panning”, completes segregation by adherence of N-CAM positive cells to a dish precoated with secondary IgG antibody whereas in the other separation proceeds by the use of commercially available IgG-coated magnetic beads. Results indicate magnetic bead separation to be more efficient than panning if the beads are precoated with 0.1% gelatin.

scholarly journals Effects of different quantities of antibody conjugated with magnetic nanoparticles on cell separation efficiency

Heliyon ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. e03677
Author(s):  
Amir Hossein Haghighi ◽  
Mohammad Taghi Khorasani ◽  
Zahra Faghih ◽  
Fatemeh Farjadian
Keyword(s):  
Magnetic Nanoparticles ◽  
Cell Separation ◽  
Separation Efficiency

scholarly journals Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis

Micromachines ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 699
Author(s):  
Rohollah Nasiri ◽  
Amir Shamloo ◽  
Javad Akbari ◽  
Peyton Tebon ◽  
Mehmet R. Dokmeci ◽  
...  
Keyword(s):  
Numerical Simulation ◽  
Angular Velocity ◽  
Microfluidic Device ◽  
Cell Separation ◽  
High Efficiency ◽  
Separation Efficiency ◽  
White Blood Cells ◽  
Cell Lysis ◽  
Target Cells ◽  
Separation Unit

Separation of circulating tumor cells (CTCs) from blood samples and subsequent DNA extraction from these cells play a crucial role in cancer research and drug discovery. Microfluidics is a versatile technology that has been applied to create niche solutions to biomedical applications, such as cell separation and mixing, droplet generation, bioprinting, and organs on a chip. Centrifugal microfluidic biochips created on compact disks show great potential in processing biological samples for point of care diagnostics. This study investigates the design and numerical simulation of an integrated microfluidic device, including a cell separation unit for isolating CTCs from a blood sample and a micromixer unit for cell lysis on a rotating disk platform. For this purpose, an inertial microfluidic device was designed for the separation of target cells by using contraction–expansion microchannel arrays. Additionally, a micromixer was incorporated to mix separated target cells with the cell lysis chemical reagent to dissolve their membranes to facilitate further assays. Our numerical simulation approach was validated for both cell separation and micromixer units and corroborates existing experimental results. In the first compartment of the proposed device (cell separation unit), several simulations were performed at different angular velocities from 500 rpm to 3000 rpm to find the optimum angular velocity for maximum separation efficiency. By using the proposed inertial separation approach, CTCs, were successfully separated from white blood cells (WBCs) with high efficiency (~90%) at an angular velocity of 2000 rpm. Furthermore, a serpentine channel with rectangular obstacles was designed to achieve a highly efficient micromixer unit with high mixing quality (~98%) for isolated CTCs lysis at 2000 rpm.

scholarly journals Proposal of a Deformable Erythrocyte Model and Numerical Analysis of Shear Flow of Blood

2001 ◽  
Vol 44 (4) ◽  
pp. 964-971 ◽  
Author(s):  
Takuji ISHIKAWA ◽  
Nobuyoshi KAWABATA ◽  
Motoyoshi TACHIBANA
Keyword(s):  
Numerical Analysis ◽  
Shear Flow

A101 A Numerical Analysis of a Capsule Containing Multiple Small Capsules in Simple Shear Flow

2013 ◽  
Vol 2013.24 (0) ◽  
pp. 1-2
Author(s):  
Yuki ICHIKAWA ◽  
Yohsuke IMAI ◽  
Daiki MATSUNAGA ◽  
Takuji ISHIKAWA ◽  
Takami YAMAGUCHI
Keyword(s):  
Numerical Analysis ◽  
Shear Flow ◽  
Simple Shear ◽  
Simple Shear Flow

Numerical Analysis of Liquid Sheet Instability in Shear Flow

2016 ◽  
Vol 2016.52 (0) ◽  
pp. 312
Author(s):  
Hiroto ENDO ◽  
Yosuke SUENAGA ◽  
Hideki YANAOKA
Keyword(s):  
Numerical Analysis ◽  
Shear Flow ◽  
Liquid Sheet
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