Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni
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Automated ChIPmentation procedure is a convenient alternative to native chromatin immunoprecipitation (N-ChIP). It is now routinely used for ChIP-Seq. Using the human parasite Schistosoma mansoni, whose production requires scarifying animals and should therefore kept to a minimum, we show here that the automated ChIPmentation is suitable for limited biological material. We define as operational limit ≥20,000 cells. We also present a streamlined protocol for the preparation of ChIP input libraries.
2014 ◽
Vol 289
(10)
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pp. 7151-7163
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1986 ◽
Vol 83
(15)
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pp. 5544-5548
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1997 ◽
Vol 14
(6)
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pp. 602-610
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1996 ◽
Vol 114
(3)
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pp. 227-234
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2000 ◽
Vol 380
(1)
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pp. 46-55
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