scholarly journals Chromosome compartmentalization replacement during stem cell differentiation

2018 ◽  
Author(s):  
Y.A. Eidelman ◽  
S.V. Slanina ◽  
V.S. Pyatenko ◽  
S.G. Andreev
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Large Scale ◽  
3D Structure ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Coarse Grained ◽  
Interphase Chromosome ◽  
Coarse Grained Model ◽  
Differentiated Cells

ABSTRACTIn this paper, changes in a large-scale 3D structure of chromosomes during stem cell differentiation is studied. The polymer coarse-grained model of a human interphase chromosome is introduced which reproduces the experimental Hi-C contact maps in chromosomes 12, 17 for both embryonic stem and differentiated cells with high accuracy. Model based analysis of Hi-C data suggests a mechanism of establishment of preferential long-range chromosomal contacts and compartmentalization replacement during cell stem differentiation. The model provides the conceptual basis for integration of data on the dynamics of chromatin interactions, the 3D structure of chromosomes and gene expression during stem cell differentiation or reprogramming.

Effects of Encapsulation Microenvironment on Embryonic Stem Cell Differentiation

2008 ◽  
Author(s):  
Lulu Li ◽  
Rene Schloss ◽  
Noshir Langrana ◽  
Martin Yarmush
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Large Scale ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Cell Aggregation ◽  
Embryonic Stem Cell Differentiation ◽  
Cell Lineages

Pluripotent embryonic stem cells represent a promising renewable cell source to generate a variety of differentiated cell types. Although many investigators have described techniques to effectively differentiate stem cells into different mature cell lineages, their practicality is limited by the absence of large scale processing consideration and low yields of differentiated cells. Previously we have established a murine embryonic stem cell alginate-poly-l-lysine microencapsulation differentiation system. The three-dimensional alginate microenvironment maintains cell viability, is conducive to ES cell differentiation to hepatocyte lineage cells, and maintains differentiated cellular function. In the present work, we demonstrate that hepatocyte differentiation is mediated by cell-cell aggregation in the encapsulation microenvironment. Both cell aggregation and hepatocyte functions, such as urea and albumin secretion, as well as increased expression of cytokaratin 18 and cyp4507a, occur concomitantly with surface E-cadherin expression. Furthermore, by incorporating soluble inducers, such as retinoic acid, into the permeable microcapsule system, we demonstrate decreased cell aggregation and enhanced neuronal lineage differentiation with the expression of various neuronal specific markers, including neurofilament, A2B5, O1 and GFAP. Therefore, as a result of capsule parameter and microenvironment manipulation, we are capable of targeting cellular differentiation to both endodermal and ectodermal cell lineages.

Calcineurin A gamma and NFATc3/SRPX2 axis contribute to human embryonic stem cell differentiation

2021 ◽  
Author(s):  
Hao Chen ◽  
Yanwu Zeng ◽  
Min Shao ◽  
Hanzhi Zhao ◽  
Zhuoqing Fang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation ◽  
Calcineurin A

scholarly journals Identification of cardiac stem cells with FLK1, CD31, and VE‐cadherin expression during embryonic stem cell differentiation

2005 ◽  
Vol 19 (3) ◽  
pp. 371-378 ◽  
Author(s):  
Midori Iida ◽  
Toshio Heike ◽  
Momoko Yoshimoto ◽  
Shiro Baba ◽  
Hiraku Doi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation ◽  
Cardiac Stem Cells

scholarly journals Activation of proHGF by St14 induces mouse embryonic stem cell differentiation

2016 ◽  
Vol 7 (8) ◽  
pp. 601-605
Author(s):  
Xiaoshuang Yan ◽  
Yan Xue ◽  
Yiye Zhou ◽  
Yan Cheng ◽  
Shang Yin ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation
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