scholarly journals Whole-brain calcium imaging during physiological vestibular stimulation in larval zebrafish

2018 ◽  
Author(s):  
Geoffrey Migault ◽  
Thomas Panier ◽  
Raphaël Candelier ◽  
Georges Debrégeas ◽  
Volker Bormuth
Keyword(s):  
Virtual Environments ◽  
Functional Imaging ◽  
Light Sheet ◽  
Vestibular Stimulation ◽  
Brain Response ◽  
Whole Brain ◽  
Larval Zebrafish ◽  
In Vivo Functional Imaging ◽  
First Time

AbstractDuring in vivo functional imaging, animals are head-fixed and thus deprived from vestibular inputs, which severely hampers the design of naturalistic virtual environments. To overcome this limitation, we developed a miniaturized ultra-stable light-sheet microscope that can be dynamically rotated during imaging along with a head-restrained zebrafish larva. We demonstrate that this system enables whole-brain functional imaging at single-cell resolution under controlled vestibular stimulation. We recorded for the first time the dynamic whole-brain response of a vertebrate to physiological vestibular stimulation. This development largely expands the potential of virtual-reality systems to explore complex multisensory-motor integration in 3D.

Dual-region in vivo Functional Imaging with a Spatial Light Modulator

2015 ◽  
Author(s):  
Weijian Yang ◽  
Jae-eun Kang Miller ◽  
Luis Carrillo-Reid ◽  
Rafael Yuste ◽  
Darcy S. Peterka
Keyword(s):  
Functional Imaging ◽  
Spatial Light Modulator ◽  
Light Modulator ◽  
In Vivo Functional Imaging

scholarly journals A brainstem integrator for self-localization and positional homeostasis

2021 ◽  
Author(s):  
En Yang ◽  
Maarten F Zwart ◽  
Mikail Rubinov ◽  
Ben James ◽  
Ziqiang Wei ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Optic Flow ◽  
Inferior Olive ◽  
Brain Regions ◽  
Neural Pathway ◽  
Whole Brain ◽  
Water Currents ◽  
Larval Zebrafish ◽  
Integrate Optic ◽  
As If

To accurately track self-location, animals need to integrate their movements through space. In amniotes, representations of self-location have been found in regions such as the hippocampus. It is unknown whether more ancient brain regions contain such representations and by which pathways they may drive locomotion. Fish displaced by water currents must prevent uncontrolled drift to potentially dangerous areas. We found that larval zebrafish track such movements and can later swim back to their earlier location. Whole-brain functional imaging revealed the circuit enabling this process of positional homeostasis. Position-encoding brainstem neurons integrate optic flow, then bias future swimming to correct for past displacements by modulating inferior olive and cerebellar activity. Manipulation of position-encoding or olivary neurons abolished positional homeostasis or evoked behavior as if animals had experienced positional shifts. These results reveal a multiregional hindbrain circuit in vertebrates for optic flow integration, memory of self-location, and its neural pathway to behavior.

scholarly journals Minimally invasive microendoscopy system for in vivo functional imaging of deep nuclei in the mouse brain

2015 ◽  
Vol 6 (11) ◽  
pp. 4546 ◽  
Author(s):  
Miriam E. Bocarsly ◽  
Wan-chen Jiang ◽  
Chen Wang ◽  
Joshua T. Dudman ◽  
Na Ji ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Mouse Brain ◽  
Functional Imaging ◽  
In Vivo Functional Imaging

scholarly journals Dual-slit confocal light sheet microscopy for in vivo whole-brain imaging of zebrafish

2015 ◽  
Vol 6 (5) ◽  
pp. 1797 ◽  
Author(s):  
Zhe Yang ◽  
Li Mei ◽  
Fei Xia ◽  
Qingming Luo ◽  
Ling Fu ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Light Sheet ◽  
Whole Brain ◽  
Light Sheet Microscopy

scholarly journals In vivo functional imaging of human cone photoreceptors

2007 ◽  
Vol 15 (24) ◽  
pp. 16141 ◽  
Author(s):  
Ravi S. Jonnal ◽  
Jungtae Rha ◽  
Yan Zhang ◽  
Barry Cense ◽  
Weihua Gao ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Cone Photoreceptors ◽  
In Vivo Functional Imaging
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