scholarly journals PCR with degenerate primers containing deoxyinosine fails with Pfu DNA polymerase.

1993 ◽  
Vol 2 (4) ◽  
pp. 346-347 ◽  
Author(s):  
T Knittel ◽  
D Picard
Keyword(s):  
Dna Polymerase ◽  
Degenerate Primers ◽  
Pfu Dna Polymerase

scholarly journals Engineered split in Pfu DNA polymerase fingers domain improves incorporation of nucleotide  -phosphate derivative

2010 ◽  
Vol 39 (5) ◽  
pp. 1801-1810 ◽  
Author(s):  
C. J. Hansen ◽  
L. Wu ◽  
J. D. Fox ◽  
B. Arezi ◽  
H. H. Hogrefe
Keyword(s):  
Dna Polymerase ◽  
Pfu Dna Polymerase

An Improved Phage Display Antibody Cloning System Using Newly Designed PCR Primers Optimized for Pfu DNA Polymerase

1995 ◽  
Vol 117 (6) ◽  
pp. 1218-1227 ◽  
Author(s):  
Hachiro I. Yamanaka ◽  
Yasuyuki Kirii ◽  
Hiroshi Ohmoto
Keyword(s):  
Phage Display ◽  
Dna Polymerase ◽  
Pcr Primers ◽  
Cloning System ◽  
Pfu Dna Polymerase ◽  
Phage Display Antibody

Purification of recombinant Pfu DNA polymerase using a new JK110 resin

2006 ◽  
Vol 23 (4) ◽  
pp. 607-609 ◽  
Author(s):  
Zhanghui Sun ◽  
Jin Cai
Keyword(s):  
Dna Polymerase ◽  
Pfu Dna Polymerase ◽  
Jk110 Resin

Molecular cloning and expression of Pfu DNA polymerase gene and its application in long-distance PCR

1998 ◽  
Vol 43 (10) ◽  
pp. 863-867 ◽  
Author(s):  
Bing Li ◽  
Cheng Gu ◽  
Jindong Zhao
Keyword(s):  
Molecular Cloning ◽  
Dna Polymerase ◽  
Cloning And Expression ◽  
Polymerase Gene ◽  
Long Distance ◽  
Dna Polymerase Gene ◽  
Long Distance Pcr ◽  
Pfu Dna Polymerase

A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification

2019 ◽  
Author(s):  
Prabu Siva Sankar ◽  
Marimuthu Citartan ◽  
Aminah Ahmed Siti ◽  
Boris V. Skryabin ◽  
Timofey S. Rozhdestvensky ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Expression System ◽  
Pcr Amplification ◽  
The Other ◽  
High Fidelity ◽  
Purification Method ◽  
Simple Method ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Pfu Dna Polymerase

  Background and Objectives: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3´ to 5´ exonucle- ase (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs). This study was aimed to express and purify Pfu DNA polymerase in a bacterial expression system under a simple purification method. Materials and Methods: Pfu polymerase gene sequence, derived from Pyrocuccus furiosus (Pfu) genomic DNA, was cloned and overexpressed in E. coli BL21 (DE3) pLysS. Upon overexpression, bacterial lysate containing the Pfu DNA polymerase was heated at 94°C for 5 minutes. Pfu DNA polymerase having high thermal stability was retained while the other bacterial proteins were denatured. The resulting thermo stable Pfu DNA polymerase was separated from the other debris of the dena- tured proteins by simple centrifugation. Results: The enzymatic activity of the resulting Pfu DNA polymerase was estimated by comparing with the commer- cial Pfu DNA Polymerases. An estimated 50000 units of functional Pfu DNA polymerase was produced from a 400 ml culture. Conclusion: The in-house produced Pfu DNA Polymerase could be used for routine amplification that requires high-fidelity such as cloning and DNA sequencing.

Purification of recombinant Pfu DNA polymerase by double step affinity chromatography

2014 ◽  
pp. 42-47 ◽  
Author(s):  
А. Mussakhmetov ◽  
◽  
A. Nurmagambetova ◽  
S. Abeldenov ◽  
B. Khassenov
Keyword(s):  
Affinity Chromatography ◽  
Dna Polymerase ◽  
Double Step ◽  
Pfu Dna Polymerase

scholarly journals Site-directed mutagenesis using Pfu DNA polymerase and T4 DNA ligase

BioTechniques ◽  
2005 ◽  
Vol 38 (6) ◽  
pp. 864-868 ◽  
Author(s):  
Yair Adereth ◽  
Kristen J. Champion ◽  
Tien Hsu ◽  
Vincent Dammai
Keyword(s):  
Dna Polymerase ◽  
Site Directed Mutagenesis ◽  
Dna Ligase ◽  
Directed Mutagenesis ◽  
T4 Dna Ligase ◽  
Pfu Dna Polymerase

A method for filling in the cohesive ends of double-stranded DNA using Pfu DNA polymerase

2005 ◽  
Vol 42 (3) ◽  
pp. 223 ◽  
Author(s):  
Xin Li ◽  
Shaohui Yang ◽  
Dongfeng Ding ◽  
Jianhua Hou ◽  
Zhaoxia Jin ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Double Stranded Dna ◽  
Pfu Dna Polymerase

scholarly journals A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering

2010 ◽  
Vol 10 (1) ◽  
pp. 21 ◽  
Author(s):  
Morten HH Nørholm
Keyword(s):  
Dna Polymerase ◽  
Dna Engineering ◽  
Pfu Dna Polymerase
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