Identification of Differential Genes by Suppression Subtractive Hybridization: III. PCR Amplification of Differentially Presented DNAs

2008 ◽  
Vol 2008 (8) ◽  
pp. pdb.prot4857-pdb.prot4857
Author(s):  
D. V. Rebrikov
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Pcr Amplification ◽  
Subtractive Hybridization ◽  
Differential Genes

scholarly journals Use of suppression subtractive hybridization to examine the accessory genome of the Liverpool cystic fibrosis epidemic strain of Pseudomonas aeruginosa

2006 ◽  
Vol 55 (6) ◽  
pp. 677-688 ◽  
Author(s):  
Catherine H. M. Smart ◽  
Martin J. Walshaw ◽  
C. Anthony Hart ◽  
Craig Winstanley
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Suppression Subtractive Hybridization ◽  
Sequence Data ◽  
Pcr Amplification ◽  
Subtractive Hybridization ◽  
Epidemic Strain ◽  
Strain Pao1 ◽  
The Uk ◽  
Specific Sequences

The Liverpool epidemic strain (LES) of Pseudomonas aeruginosa has been highly successful at colonizing cystic fibrosis (CF) patients throughout the UK, has replaced previously established strains in CF patients, has caused infections of non-CF parents of CF patients, and can cause greater morbidity in CF than other strains of P. aeruginosa. Using suppression subtractive hybridization (SSH) to identify strain-specific sequences, a diagnostic test for the LES based on PCR amplification of SSH sequence PS21 had previously been developed. In this study, the SSH sequence database of LES was substantially increased, using both extension of previous sequences and new rounds of subtraction. Of 92 SSH sequences identified as present in the LES but absent from strain PAO1, 25 were assessed for prevalence amongst a strain panel consisting mainly of LES and non-LES CF isolates. Preliminary analysis of genome sequence data indicated that all SSH sequences that were LES specific or found only rarely in other strains of P. aeruginosa were present on one of three contigs. All of the SSH sequences screened were either unstable amongst LES isolates or were not completely LES specific. Rare false positives were found with the PS21 test. The authors suggest that a second PCR assay designed to detect SSH sequence LESF9 can be used to confirm the identity of the most prevalent CF epidemic lineage in the UK.

Identification of Differential Genes by Suppression Subtractive Hybridization: An Overview

2008 ◽  
Vol 2008 (8) ◽  
pp. pdb.top21-pdb.top21 ◽  
Author(s):  
D. V. Rebrikov
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Subtractive Hybridization ◽  
Differential Genes

Construction and analysis of gonad suppression subtractive hybridization library of rice field eel (Monopterus albus)

2013 ◽  
Vol 18 (1) ◽  
pp. 23-28
Author(s):  
Xiancheng QU ◽  
Xiaoli SHANG ◽  
Cui CHENG ◽  
Xuewei QU ◽  
Yong ZHANG ◽  
...  
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Rice Field ◽  
Subtractive Hybridization ◽  
Suppression Subtractive Hybridization Library ◽  
Monopterus Albus ◽  
Rice Field Eel
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