scholarly journals Protein Phosphatase 2A B'α and B'β Protect Centromeric Cohesion during Meiosis I

2019 ◽  
Vol 179 (4) ◽  
pp. 1556-1568 ◽  
Author(s):  
Yu-Lan Zhang ◽  
He Zhang ◽  
Ying-Jie Gao ◽  
Lin-Lin Yan ◽  
Xin-Yu Yu ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
Protein Phosphatase 2A ◽  
Phosphatase 2A ◽  
Meiosis I

scholarly journals Multiple pools of Protein Phosphatase 2A-B56 function to antagonize spindle assembly, promote kinetochore attachments and maintain cohesion in Drosophila Oocytes

2020 ◽  
Author(s):  
Janet K. Jang ◽  
Amy C. Gladstein ◽  
Arunika Das ◽  
Zachary L. Sisco ◽  
Kim S. McKim
Keyword(s):  
Protein Phosphatase ◽  
Protein Phosphatase 2A ◽  
Spindle Assembly ◽  
Meiotic Spindle ◽  
Aurora B ◽  
Microtubule Organizing Center ◽  
Chromosomal Passenger ◽  
Organizing Center ◽  
Phosphatase 2A ◽  
Meiosis I

AbstractMeiosis in female oocytes lack centrosomes, the major microtubule-organizing center, which makes them especially vulnerable to aneuploidy. In the acentrosomal oocytes of Drosophila, meiotic spindle assembly depends on the chromosomal passenger complex (CPC). Aurora B is the catalytic component of the CPC while the remaining subunits regulate its localization. Using an inhibitor of Aurora B activity, Binucleine 2, we found that continuous Aurora B activity is required to maintain the oocyte spindle during meiosis I, and this activity is antagonized by phosphatases acting on spindle associated proteins such as kinesins. Protein Phosphatase 2A (PP2A) exists in two varieties, B55 and B56. While both antagonize Aurora B, B55 has only minor roles in meiosis I spindle function. The B56 subunit is encoded by two partially redundant paralogs in the Drosophila genome, wdb and wrd. Knocking down both paralogs showed that the B56 subunit is critical for maintaining sister chromatid cohesion, establishing end-on microtubule attachments, and the metaphase I arrest in oocytes. We found that WDB recruitment to the centromeres depends on BUBR1, MEI-S332, and kinetochore protein SPC105R. While BUBR1 has been shown previously to stabilize microtubule attachments in Drosophila oocytes, only SPC105R is required for cohesion maintenance during meiosis I. We propose that SPC105R promotes cohesion maintenance by recruiting two proteins that recruit PP2A, MEI-S332, and the Soronin homolog Dalmatian.

Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I

Nature ◽  
2006 ◽  
Vol 441 (7089) ◽  
pp. 53-61 ◽  
Author(s):  
Christian G. Riedel ◽  
Vittorio L. Katis ◽  
Yuki Katou ◽  
Saori Mori ◽  
Takehiko Itoh ◽  
...  
Keyword(s):  
Sister Chromatid ◽  
Protein Phosphatase ◽  
Protein Phosphatase 2A ◽  
Sister Chromatid Cohesion ◽  
Chromatid Cohesion ◽  
Phosphatase 2A ◽  
Meiosis I
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