Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein

Matrix porin, the ompF gene product of E. coli, has been the object of a electron crystallographic study of its pore geometry in an attempt to understand its function as a membrane molecular sieve. Three polymorphic forms have been found for two-dimensional crystals reconstituted in phospholipid, two hexagonal forms with different lipid content and an orthorhombic form coexisting with and similar to the hexagonal form found after lipid loss. In projection these have been shown to retain the same three-fold pore triplet geometry and analyses of three-dimensional data reveal that the small hexagonal and orthorhombic polymorphs have similar structure as well as unit cell spacings.

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Structural genomics in the Spotlight

PSI Structural Genomics Knowledgebase ◽

10.1038/nw_psisgkb.2010.15 ◽

2010 ◽

Author(s):

Margaret Gabanyi ◽

Torsten Schwede

Keyword(s):

Structural Genomics

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Structural Genomics, Part A

10.1016/s0065-3233(07)x7500-4 ◽

2008 ◽

Keyword(s):

Structural Genomics

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A Crystallographic Study of Bis[S-benzyl-5-bromo-2-oxoindolin-3-ylidenemethanehydrazonthioate] Disulfide Solvated with Dimethylsulfoxide

Scientific Research Journal ◽

10.24191/srj.v9i2.9410 ◽

2012 ◽

Vol 9 (2) ◽

pp. 87

Author(s):

Mohd Abdul Fatah Abdul Manan ◽

M. Ibrahim M. Tahir ◽

Karen A. Crouse ◽

Fiona N.-F. How ◽

David J. Watkin

Keyword(s):

Crystal Structure ◽

Hydrogen Bonds ◽

Solvent Molecule ◽

Site Occupancy ◽

Unit Cell Parameters ◽

Intermolecular Hydrogen Bonds ◽

Triclinic Space Group ◽

Cell Parameters ◽

Crystallographic Study ◽

Thiol Form

The crystal structure of the title compound has been determined. The compound crystallized in the triclinic space group P -1, Z = 2, V = 1839 .42( 18) A3 and unit cell parameters a= 11. 0460( 6) A, b = 13 .3180(7) A, c=13. 7321 (8) A, a = 80.659(3 )0, b = 69 .800(3 )0 and g = 77 .007 (2)0 with one disordered dimethylsulfoxide solvent molecule with the sulfur and oxygen atoms are distributed over two sites; S101/S102 [site occupancy factors: 0.6035/0.3965] and 0130/0131 [site occupancy factor 0.3965/0.6035]. The C22-S2 l and C 19-S20 bond distances of 1. 779(7) A and 1. 788(8) A indicate that both of the molecules are connected by the disulfide bond [S20-S21 2.055(2) A] in its thiol form. The crystal structure reveals that both of the 5-bromoisatin moieties are trans with respect to the [S21-S20 and CI 9-Nl 8] and [S20-S21 and C22-N23] bonds whereas the benzyl group from the dithiocarbazate are in the cis configuration with respect to [S21-S20 and C19-S44] and [S20-S21 and C22-S36] bonds. The crystal structure is further stabilized by intermolecular hydrogen bonds of N9-H35···O16 formed between the two molecules and N28-H281 ···O130, N28-H281 ···O131 and C4 l-H4 l l ···O 131 with the solvent molecule.

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