The 1.5t/h algae and bacteria wastewater with high concentration was treated by hydroxyl radical (OH) produced by a strong ionization discharge. In this paper, the feasibility of kill bacteria and alga by OH was investigated, and effects of OH concentration, treatment time and pollution initial concentration on degrading microorganisms by OH were investigated in detail. The results indicated that the treatment process was effective in the degradation of the bacteria and alga at the initial pH value of 7.1, OH dosage of 2.33mg/L, the initial microcystis aeruginosa concentration of 106/mL and the initial bacterial dosage of 106cfu/mL, the bacteria and alga degradation rate reached 99.99% at 303K after 15min treatment. The results provided a theoretical basis for the OH treatment of microbial contamination in drinking water in large-scale.