The Recognition of Human 60-kDa Ro Ribonucleoprotein Particles by Antibodies Associated With Cutaneous Lupus and Neonatal Lupus

Xenopus laevis oocytes have been used to determine the intracellular localization of components of Ro ribonucleoprotein particles (Ro RNPs) and to study the assembly of these RNA-protein complexes. Microinjection of the protein components of human Ro RNPs, i.e., La, Ro60, and Ro52, in X. laevis oocytes showed that all three proteins are able to enter the nucleus, albeit with different efficiencies. In contrast, the RNA components of human Ro RNPs (the Y RNAs) accumulate in the X. laevis cytoplasm upon injection. Localization studies performed at low temperatures indicated that both nuclear import of Ro RNP proteins and nuclear export of Y RNAs are mediated by active transport mechanisms. Immunoprecipitation experiments using monospecific anti-La and anti-Ro60 antibodies showed that the X. laevis La and Ro60 homologues were cross-reactive with the respective antibodies and that both X. laevis proteins were able to interact with human Y1 RNA. Further analyses indicated that: (a) association of X. laevis La and Ro60 with Y RNAs most likely takes place in the nucleus; (b) once formed, Ro RNPs are rapidly exported out of the nucleus; and (c) the association with La is lost during or shortly after nuclear export.

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Cutaneous lupus in infancy and childhood

Lupus ◽

10.1177/0961203310370347 ◽

2010 ◽

Vol 19 (9) ◽

pp. 1112-1117 ◽

Cited By ~ 22

Author(s):

LA Lee

Keyword(s):

Clinical Findings ◽

Endogenous Development ◽

Neonatal Lupus ◽

Infancy And Childhood ◽

Cutaneous Lupus

Cutaneous lupus may occur in infancy as transient lesions associated with and probably caused by maternal autoantibodies, or later in childhood, associated with the endogenous development of autoimmunity. In this review, clinical findings, diagnosis, management, and pathogenesis of neonatal lupus are discussed, and the management of cutaneous lupus in children is detailed. Lupus (2010) 19, 1112—1117.

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Cutaneous neonatal lupus with cutis marmorata telangiectatica congenita-like lesions

Anais Brasileiros de Dermatologia ◽

10.1590/abd1806-4841.20131832 ◽

2013 ◽

Vol 88 (3) ◽

pp. 428-431 ◽

Cited By ~ 9

Author(s):

Flavia Trevisan ◽

Paulo Rowilson Cunha ◽

Clovis Antonio Lopes Pinto ◽

Fernanda Gomes Cattete

Keyword(s):

Rare Disease ◽

Heart Block ◽

Congenital Heart ◽

Clinical Manifestations ◽

Congenital Heart Block ◽

Neonatal Lupus ◽

Cutaneous Manifestations ◽

Cutis Marmorata Telangiectatica Congenita ◽

The Face ◽

Cutaneous Lupus

Neonatal lupus is a rare disease caused by the transplacental transfer of maternal autoantibodies to the foetus, characterized by transient clinical manifestations such as cutaneous, haematological, and hepatobiliary events or permanent such as congenital heart block. The typical cutaneous manifestations include erythematous, scaly, annular or arched lesions on the face, with slight central atrophy and photosensitivy, clinically and histologically similar to subacute cutaneous lupus. However, in some cases, the lesions may resemble those in cutis marmorata telangiectatica congenita, although this phenomenon is rare and only eight such cases have been reported to date. We report a case of cutaneous neonatal lupus with atypical lesions on the limbs, which had a reddish-purple marbled appearance, resembling the lesions in cutis marmorata telangiectatica congenita.

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