Plastic Embedding of Cutaneous Specimens for Light Microscopy using Araldite Epoxy Resin

Abstract Semithin and thin sections of glutaraldehyde-fixed, epoxy resin-embedded animal tissues were treated with solutions of oxidized p-phenylenediamine (PPD). This method is suitable to reveal the general morphology of tissues in light microscopy, showing a high staining degree in some polyanion containing components. Posttreatments of thin sections with gold chloride solutions give consider able electron opacity in PPD positive structures.

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Plastic Embedding for Light Microscopy

Theory and Practice of Histological Techniques ◽

10.1016/b978-0-443-10279-0.50036-1 ◽

2008 ◽

pp. 585-599

Author(s):

Neil M. Hand

Keyword(s):

Light Microscopy ◽

Plastic Embedding

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Plastic Embedding for Light Microscopy

Laboratory Procedures and their Applications ◽

10.1016/b978-0-12-715001-7.50082-2 ◽

1984 ◽

pp. 684-688 ◽

Cited By ~ 1

Author(s):

Claudia Botti ◽

Indra K. Vasil

Keyword(s):

Light Microscopy ◽

Plastic Embedding

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Plastic embedding of transbronchial biopsy specimens for light microscopy.

Journal of Clinical Pathology ◽

10.1136/jcp.32.12.1294 ◽

1979 ◽

Vol 32 (12) ◽

pp. 1294-1298 ◽

Cited By ~ 3

Author(s):

C W Edwards ◽

A Krypczyk ◽

A Brownhill

Keyword(s):

Light Microscopy ◽

Transbronchial Biopsy ◽

Biopsy Specimens ◽

Plastic Embedding

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Rapid Specimen Preparation Using Microwave Energy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119065 ◽

1985 ◽

Vol 43 ◽

pp. 448-449 ◽

Cited By ~ 1

Author(s):

F. Baldwin ◽

M.S. Diocee

Keyword(s):

Electron Microscopy ◽

Light Microscopy ◽

Epoxy Resin ◽

Wistar Rats ◽

Microwave Energy ◽

Rapid Diagnosis ◽

Specimen Preparation ◽

Rapid Preparation ◽

Male Wistar Rats ◽

Kidney Liver

In recent years the feasibility of using microwave energy to fix tissue and expedite histologic staining has been tested. The technique has proven to be particularly valuable for shortening staining time of light microscopy preparations', thus enabling more rapid diagnosis which may be critical to patient care.Rapid preparation of tissue for electron microscopy is often desirable in diagnostic ultrastructural pathology but with techniques commonly in use several hours may elapse before epoxy resin blocks reach a consistency suitable for ultrathin sectioning.In this laboratory, microwave energy has been used to polymerise epoxy resin and the usefulness of the technique has been evaluated.Kidney, liver and lung from male Wistar rats were fixed in phosphate buffered glutaraldehyde, cacodylate buffered glutaraldehyde and universal fixative.

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