scholarly journals Time-lapse imaging system with shell-less culture chamber

2014 ◽  
Vol 56 (4) ◽  
pp. 305-309 ◽  
Author(s):  
Jun-ichi Funahashi ◽  
Harukazu Nakamura
Keyword(s):  
Imaging System ◽  
Time Lapse ◽  
Culture Chamber ◽  
Time Lapse Imaging

P–201 The beneficial effects of ZP-free culture on cytoplasmic fragmentation in human embryos. : An innovative trial using 3PN zygotes

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
M Sugishima ◽  
K Yumoto ◽  
T Shimura ◽  
Y Mio
Keyword(s):  
Embryo Development ◽  
Imaging System ◽  
Culture Method ◽  
Cumulus Cells ◽  
Time Lapse ◽  
Human Embryos ◽  
Beneficial Effects ◽  
Molecular Biological Analysis ◽  
A Prospective Study ◽  
Time Lapse Imaging

Abstract Study question Is it possible to culture ZP-free embryos to eliminate perivitelline threads, which are known to be involved in generating cytoplasmic fragments at the first cleavage? Summary answer ZP-free culturing, an innovative system that decreases the amount of cytoplasmic fragments without disrupting the blastomeres, using incubators with time-lapse imaging. What is known already A study in 2017 observed perivitelline threads in more than 50% of cleavage-stage human embryos using time-lapse imaging, and the rate of cytoplasmic fragmentation (at the first cleavage) was significantly decreased in embryos without perivitelline threads (P < 0.001). While it has been proposed that perivitelline threads play an important role in crosslinking the cumulus cells and oocyte during maturation, the mechanism underlying such a role remains unclear. It is also unknown whether the threads still function in mature MII oocytes. Study design, size, duration A prospective study was conducted using 2,852 normal (2PN/2PB) embryos from c-IVF/ICSI and 113 abnormal (3PN) embryos obtained from c-IVF between 2017 and 2019. The zona pellucida (ZP) of 71 abnormal embryos was removed at the pronuclear stage (“ZP-free”), and the rest (n = 42) were cultured as “ZP-intact”. Normal and abnormal embryos were cultured for five days in bench-top incubators (MINC, COOK) and an incubator equipped with a time-lapse imaging system. Participants/materials, setting, methods Embryos used in this study were donated by 412 couples who underwent c-IVF cycles in our clinic between 2017 and 2019. For ZP removal, 3PN embryos were placed in 0.125M sucrose-containing HEPES media drops to reduce the ooplasm size. Then, ooplasms were completely separated from ZPs by a laser and pipetting. Embryo development and morphology of the three groups (normal, ZP-intact and ZP-free abnormal) were compared based on the degree of cytoplasmic fragmentation. Main results and the role of chance The first cleavage occurred in 97.8% (n = 2,790/2,852) of 2PN/2PB, 83.3% (n = 35/42) of ZP-intact 3PN and 97.2% (n = 69/71) of ZP-free 3PN. Normal (2PN/2PB), ZP-intact and ZP-free 3PN embryos were classified into three groups based on the modified Veeck’s criteria thus: <20% fragmented compared to the total volume of cytoplasm at the first cleavage (Grade 1 and 2, Good); 20–39% fragmented (Grade 3, Fair) and ≧40% fragmented (Grade 4, Poor). Of 69 cleaved ZP-free 3PN embryos, 68.1% (n = 47) showed less than 20% fragments which was significantly higher than 2PN/2PB (43.7%, n = 1,218/2,790) and ZP-intact 3PN (45.7%, n = 16/35; P < 0.05). Furthermore, 24.6% (n = 17/69) of ZP-free 3PN embryos showed 20–39% fragments which was significantly lower than 2PN/2PB (45.9%, n = 1,281/2,790; P < 0.05). In addition, 50.7% of ZP-free 3PN embryos (n = 36) developed to the morula stage after the third cleavage, and 29.6% (n = 21) formed blastocoel and became blastocysts. Thus, removing the ZP before the first cleavage did not adversely affect embryo development and decreased the cytoplasmic fragmentation. Limitations, reasons for caution Due to ethical and clinical limitations, we only examined abnormally fertilized embryos in this study. Moreover, since the relationship between the perivitelline threads and cytoplasmic fragments is unclear, we plan to conduct molecular biological analysis of the perivitelline threads in further studies. Wider implications of the findings: This study revealed that ZP is not always necessary after the pronuclear stage because ZP-free embryos studied herein developed normally and maintained cell adhesion well. This innovative culture method might provide the breakthrough needed for patients to improve embryo quality who obtain embryos with severe fragmentation caused by perivitelline threads. Trial registration number Not applicable

Advantages of a Time-Lapse Imaging System in Assisted Reproductive Technology

2015 ◽  
Vol 32 (4) ◽  
pp. 133-142
Author(s):  
Kazuhiro Matsuda ◽  
Masanobu Ito ◽  
Yumiho Yamaguchi ◽  
Fujiyo Matsushita ◽  
Hiromi Kawasaki ◽  
...  
Keyword(s):  
Assisted Reproductive Technology ◽  
Imaging System ◽  
Time Lapse ◽  
Reproductive Technology ◽  
Time Lapse Imaging

Omnidirectional Time-Lapse Imaging System for Egg-in-Cube Platform

2019 ◽  
Vol 2019.31 (0) ◽  
pp. 1C23
Author(s):  
Hatsushi TANIGUCHI ◽  
Tomohiro KAWAHARA
Keyword(s):  
Imaging System ◽  
Time Lapse ◽  
Time Lapse Imaging

Preliminary morphokinetic annotation data for early equine embryo development in vitro using a time-lapse imaging system

2016 ◽  
Vol 41 ◽  
pp. 72
Author(s):  
Karen Schnauffer ◽  
Niamh Lewis ◽  
Stephen Troup ◽  
Dai Grove-White ◽  
Caroline McG. Argo
Keyword(s):  
Embryo Development ◽  
Imaging System ◽  
Time Lapse ◽  
Annotation Data ◽  
Development In Vitro ◽  
Time Lapse Imaging

scholarly journals SERUM ANTI-MÜLLERIAN HORMONE CONCENTRATIONS IMPACTS NOT ONLY THE QUANTITY, BUT ALSO THE QUALITY OF THE OVARIAN RESERVE: THE IMPORTANCE OF TIME-LAPSE IMAGING SYSTEM

2021 ◽  
Vol 116 (3) ◽  
pp. e243
Author(s):  
Daniela Paes de Almeida Ferreira Braga ◽  
Amanda Souza Setti ◽  
Patricia Guilherme ◽  
Livia S. Vingris ◽  
Rodrigo R. Provenza ◽  
...  
Keyword(s):  
Ovarian Reserve ◽  
Imaging System ◽  
Time Lapse ◽  
Time Lapse Imaging

scholarly journals Euploidy is not predictable by time lapse imaging system with either blastomere or trophectoderm biopsies

2015 ◽  
Vol 104 (3) ◽  
pp. e314-e315
Author(s):  
F. Li ◽  
M. Urich ◽  
J.W. Ayers ◽  
M.I. Abuzeid ◽  
I. Khan
Keyword(s):  
Imaging System ◽  
Time Lapse ◽  
Time Lapse Imaging

High-Resolution, Intravital 4D Confocal Time-Lapse Imaging in Avian Embryos Using a Teflon Culture Chamber Design: Figure 1.

2007 ◽  
Vol 2007 (7) ◽  
pp. pdb.prot4790 ◽  
Author(s):  
Paul A. Rupp ◽  
Paul M. Kulesa
Keyword(s):  
High Resolution ◽  
Time Lapse ◽  
Culture Chamber ◽  
Avian Embryos ◽  
Chamber Design ◽  
Time Lapse Imaging

HIGH OOCYTE IMMATURITY RATES IMPACT EMBRYO MORPHOKINETICS: LESSONS OF TIME-LAPSE IMAGING SYSTEM

2021 ◽  
Vol 116 (3) ◽  
pp. e235
Author(s):  
Amanda Souza Setti ◽  
Daniela Paes de Almeida Ferreira Braga ◽  
Patricia Guilherme ◽  
Livia S. Vingris ◽  
Rodrigo R. Provenza ◽  
...  
Keyword(s):  
Imaging System ◽  
Time Lapse ◽  
Time Lapse Imaging

Evaluation of the morphokinetic parameters and development of pre‐implantation embryos obtained by testicular, epididymal and ejaculate spermatozoa using time‐lapse imaging system

Andrologia ◽  
2018 ◽  
Vol 51 (4) ◽  
pp. e13217
Author(s):  
Ali Buran ◽  
Pinar Tulay ◽  
Nurten Dayıoğlu ◽  
Mustafa Emre Bakircioglu ◽  
Mustafa Bahceci ◽  
...  
Keyword(s):  
Imaging System ◽  
Time Lapse ◽  
Morphokinetic Parameters ◽  
Time Lapse Imaging
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