Real‐time molecular characterization of air pollutants in a Hong Kong residence: Implication of indoor source emissions and heterogeneous chemistry

Indoor Air ◽  
2021 ◽  
Author(s):  
Xiaopu Lyu ◽  
Yunxi Huo ◽  
Jin Yang ◽  
Dawen Yao ◽  
Kaimin Li ◽  
...  
Keyword(s):  
Hong Kong ◽  
Real Time ◽  
Molecular Characterization ◽  
Air Pollutants ◽  
Heterogeneous Chemistry

scholarly journals Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria

2020 ◽  
Vol 9 (1) ◽  
pp. 54
Author(s):  
Salem Belkessa ◽  
Daniel Thomas-Lopez ◽  
Karim Houali ◽  
Farida Ghalmi ◽  
Christen Rune Stensvold
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Specific Pcr ◽  
Stool Samples ◽  
Pcr Targeting ◽  
Assemblage B

The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data.

Molecular Characterization of Prader–Willi Syndrome by Real-Time PCR

2008 ◽  
Vol 12 (2) ◽  
pp. 319-324 ◽  
Author(s):  
Teresa Munce ◽  
Robert Simpson ◽  
Francis Bowling
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Prader Willi Syndrome

Biochemical and molecular characterization of dopa-responsive dystonias in Hong Kong Chinese

2010 ◽  
Vol 411 (11-12) ◽  
pp. 909-910
Author(s):  
C.M. Mak ◽  
T.S. Siu ◽  
S. Tam ◽  
C.W. Lam ◽  
Y.W. Chan ◽  
...  
Keyword(s):  
Hong Kong ◽  
Molecular Characterization ◽  
Hong Kong Chinese

scholarly journals Molecular Characterization of the 2011 Hong Kong Scarlet Fever Outbreak

2012 ◽  
Vol 206 (3) ◽  
pp. 341-351 ◽  
Author(s):  
H. Tse ◽  
J. Y. J. Bao ◽  
M. R. Davies ◽  
P. Maamary ◽  
H.-W. Tsoi ◽  
...  
Keyword(s):  
Hong Kong ◽  
Molecular Characterization ◽  
Scarlet Fever
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