Effect of hyperoxia and vascular occlusion on tissue oxygenation measured by near infra-red spectroscopy (InSpectra™): a volunteer study

Anaesthesia ◽  
2012 ◽  
Vol 67 (11) ◽  
pp. 1237-1241 ◽  
Author(s):  
B. Kyle ◽  
E. Litton ◽  
K. M. Ho
Keyword(s):  
Tissue Oxygenation ◽  
Vascular Occlusion ◽  
Volunteer Study ◽  
Infra Red

scholarly journals Mitochondrial oxygen monitoring with COMET: verification of calibration in man and comparison with vascular occlusion tests in healthy volunteers

2020 ◽  
Author(s):  
R. Ubbink ◽  
M. A. Wefers Bettink ◽  
W. van Weteringen ◽  
E. G. Mik
Keyword(s):  
Healthy Volunteers ◽  
Tissue Oxygenation ◽  
Vascular Occlusion ◽  
Oxygen Depletion ◽  
Arterial Oxygen ◽  
Vascular Occlusion Test ◽  
Local Pressure ◽  
Mitochondrial Oxygen Consumption ◽  
Arterial Oxygen Partial Pressure ◽  
Oxygen Monitoring

Abstract Mitochondria are the primary consumers of oxygen and therefore an important location for oxygen availability and consumption measurement. A technique has been developed for mitochondrial oxygen tension (mitoPO2) measurement, incorporated in the COMET. In contrast to most textbooks, relatively high average mitoPO2 values have been reported. The first aim of this study was to verify the validity of the COMET calibration for mitoPO2 measurements in human skin. The second aim was to compare the dynamics of mitoPO2 to several other techniques assessing tissue oxygenation. Firstly, we performed a two-point calibration. Mitochondrial oxygen depletion was achieved with vascular occlusion. A high mitoPO2 was reached by local application of cyanide. MitoPO2 was compared to the arterial oxygen partial pressure (PaO2). Secondly, for deoxygenation kinetics we compared COMET variables with the LEA O2C, SenTec OxiVenT™ and Medtronic INVOS™ parameters during a vascular occlusion test. 20 healthy volunteers were recruited and resulted in 18 datasets (2 times 9 subjects). The lowest measured mitoPO2 value per subject had a median [IQR] of 3.0 [1.0–4.0] mmHg, n = 9. After cyanide application the mitoPO2 was 94.1 mmHg [87.2–110.9] and did not differ significantly (n = 9, p = 0.5) from the PaO2 of 101.0 [98.0–106.0] mmHg. In contrast to O2C, OxiVenT™ and INVOS parameters, mitoPO2 declined within seconds with pressure on the probe. The kinetics from this decline are used to mitochondrial oxygen consumption (mitoVO2). This study validates the calibration of the COMET device in humans. For mitoVO2 measurements not only blood flow cessation but application of local pressure is of great importance to clear the measurement site of oxygen-carrying erythrocytes.

scholarly journals Comparison of muscle tissue oxygenation response curves to two time-based vascular occlusion tests: evidence of diminishing returns?

Critical Care ◽  
2009 ◽  
Vol 13 (Suppl 1) ◽  
pp. P240
Author(s):  
SJ Thomson ◽  
N Al-Subaie ◽  
M Hamilton ◽  
ML Cowan ◽  
S Musa ◽  
...  
Keyword(s):  
Muscle Tissue ◽  
Tissue Oxygenation ◽  
Vascular Occlusion ◽  
Response Curves ◽  
Diminishing Returns

scholarly journals A comparison of different modes of pneumatic compression on muscle tissue oxygenation: An intraparticipant, randomised, controlled volunteer study

2019 ◽  
Vol 47 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Sanat K Nandwana ◽  
Kwok M Ho
Keyword(s):  
Muscle Tissue ◽  
Near Infrared ◽  
Tissue Oxygenation ◽  
Lower Limbs ◽  
Mechanistic Study ◽  
Limb Muscle ◽  
Alternative Mode ◽  
Single Compartment ◽  
Hospitalised Patients ◽  
Volunteer Study

Intermittent pneumatic compression (IPC) to the lower limbs is widely used as a mechanical means to prevent deep vein thrombosis in hospitalised patients. Due to a theoretical concern about impairing blood flow, thromboembolic-deterrent stockings and IPC are considered contraindicated for patients with peripheral vascular diseases by some clinicians. This study assessed whether IPC would alter peripheral limb muscle tissue oxygenation (StO2), and whether such changes were different during 10 minutes of sequential and single-compartment compressions. Twenty volunteers were randomised to have their left or right arm treated with a sequential or single-compartment IPC for 10 minutes, using the contralateral arm without compression as an intraparticipant control. After a five-minute wash-out period, the procedure was repeated on the same arm using the alternative mode of IPC. Both hands’ thenar muscles StO2 was monitored every two minutes for 10 minutes using the same near-infrared spectroscopy StO2 monitor. Both sequential (3.5%, 95% confidence intervals (CI) 2.7–4.2; p < 0.001) and single-compartment IPC (1.6%, 95% CI 0.4–2.8; p = 0.039) significantly increased muscle StO2 within 10 minutes compared to no compression; and the increments were higher during sequential compressions compared to during single-compartment compressions (2.1%, 95% CI 0.7–3.5; p = 0.023). This mechanistic study showed that both modes of IPC increased upper limb muscle StO2 compared to no compression, but the StO2 increments were higher with the multiple-chamber sequential compressions mode. Contrary to the theoretical concern that IPC may impair peripheral limb tissue oxygenation, our results showed that IPC actually increases oxygenation of the peripheral limb muscles, especially during the sequential compressions mode.

scholarly journals Adaptability of muscle tissue oxygenation to repeated vascular occlusion

Critical Care ◽  
2009 ◽  
Vol 13 (Suppl 1) ◽  
pp. P241
Author(s):  
SJ Thomson ◽  
N Al-Subaie ◽  
M Hamilton ◽  
ML Cowan ◽  
S Musa ◽  
...  
Keyword(s):  
Muscle Tissue ◽  
Tissue Oxygenation ◽  
Vascular Occlusion

scholarly journals Effect of assuming constant tissue scattering on measured tissue oxygenation values during tissue ischemia and vascular reperfusion

2019 ◽  
Vol 127 (1) ◽  
pp. 22-30 ◽  
Author(s):  
Shane M. Hammer ◽  
Dennis M. Hueber ◽  
Dana K. Townsend ◽  
Lillie M. Huckaby ◽  
Andrew M. Alexander ◽  
...  
Keyword(s):  
Optical Properties ◽  
Near Infrared ◽  
Tissue Oxygenation ◽  
Vascular Occlusion ◽  
Tissue Oxygen Saturation ◽  
Tissue Ischemia ◽  
Rates Of Change ◽  
Tissue Optical Properties ◽  
Tissue Scattering ◽  
Nirs Data

The purpose of this study was to determine the effects of assuming constant tissue scattering properties on tissue oxygenation measurements during a vascular occlusion test (VOT). Twenty-one subjects (21.8 ± 1.9 yr) completed a VOT [1 min baseline (BL), 5 min of tissue ischemia (TI), and 3 min of vascular reperfusion (VR)]. Absolute concentrations of oxygenated heme (oxy-[heme]), deoxygenated heme (deoxy-[heme]), total heme (total [heme), tissue oxygen saturation (StO2), and heme difference [heme]diff) were measured using frequency domain near-infrared spectroscopy (FD-NIRS) while 1) continuously measuring and incorporating tissue scattering ([Formula: see text]) and 2) assuming scattering remained constant. FD-NIRS measured [Formula: see text] increased during TI at 692 nm ( P < 0.001) and decreased at 834 nm ( P < 0.001). During VR, [Formula: see text] decreased at 692 nm ( P < 0.001) and increased at 834 nm ( P < 0.001). When assuming constant scattering, oxy-[heme] was significantly less at TIpeak ( P < 0.05) while deoxy-[heme] and StO2 were significantly altered at BL, TIpeak, and VRpeak (all P < 0.001). Total [heme] did not change during the VOT. Absolute changes in deoxy-[heme], oxy-[heme], and StO2 in response to TI and VR were significantly exaggerated (all P < 0.001) and the rates of change during TI ( slope 1) and VR ( slope 2) in deoxy-[heme], oxy-[heme], StO2, and [heme]diff were significantly increased (all P < 0.05) when constant tissue scattering was assumed. These findings demonstrate the need for caution when interpreting NIRS data without continuously measuring tissue optical properties. Further, assuming tissue optical properties remain constant may have important consequences to experimental data and clinical conclusions made using NIRS. NEW & NOTEWORTHY NIRS measurements provide significant experimental and clinical insight. We demonstrate that absolute changes in tissue oxygenation measurements made with NIRS are overestimated and the kinetic responses of NIRS measurements are exaggerated by varying degrees among individuals if tissue scattering characteristics are assumed to remain constant during vascular occlusion tests.

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