Enzyme-linked immunosorbent assay (ELISA) detection of infectious pancreatic necrosis virus (IPNV) in culture fluids and tissue homogenates of the rainbow trout, Salmo gairdneri Richardson

1988 ◽  
Vol 11 (3) ◽  
pp. 225-235 ◽  
Author(s):  
L. RODAK ◽  
Z. POSPISIL ◽  
J. TOMANEK ◽  
T. VESELY ◽  
T. OBR ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Immunosorbent Assay ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Salmo Gairdneri ◽  
Enzyme Linked Immunosorbent Assay ◽  
Necrosis Virus ◽  
Tissue Homogenates ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Susceptibility of Trouts of Different Species and Origins to Various Isolates of Infectious Pancreatic Necrosis Virus

1982 ◽  
Vol 39 (12) ◽  
pp. 1580-1584 ◽  
Author(s):  
A. Silim ◽  
M. A. S. Y. Elazhary ◽  
A. Lagacé
Keyword(s):  
Rainbow Trout ◽  
Brook Trout ◽  
Pancreatic Necrosis ◽  
Lake Trout ◽  
Infectious Pancreatic Necrosis Virus ◽  
Infected Fish ◽  
Salmo Gairdneri ◽  
Necrosis Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

We investigated the susceptibility of trouts of different species and origins to infectious pancreatic necrosis virus (IPNV) and the pathogenicity of three strains of IPNV for brook trout (Salvelinus fontinalis) of various origins and lake trout (Salvelinus namaycush) of a single origin. Fish were inoculated by immersion in water containing 105 PFU of virus/mL for 6 h. Susceptibility to IPNV infection was assessed by counting dead fish over a period of 21 d after infection and on histological lesions in the pancreas, kidney, and intestine of the infected fish. Different species of trouts had different susceptibility to IPNV strain 3865. Brook trout had the highest mortality followed by rainbow trout (Salmo gairdneri), whereas the least mortality occurred in lake trout. Brook trout from Crowford, Nebraska, were more than twice as susceptible as the same species from Baldwin Mills, Quebec. Rainbow trout also varied in susceptibility as a result of origin. Virus isolate 3B, originally isolated from chain pickerel (Esox niger), was less virulent than isolates 4495 or 3865 (both from trout). Hatcheries that use water from sources containing pickerel may increase their chances of IPN infection.Key words: trout species, infectious pancreatic necrosis virus, resistance

Isolation of Infectious Pancreatic Necrosis Virus from the Blood and Blood Components of Experimentally Infected Trout

1982 ◽  
Vol 39 (1) ◽  
pp. 225-228 ◽  
Author(s):  
Robert N. Swanson ◽  
James H. Gillespie
Keyword(s):  
Rainbow Trout ◽  
Brook Trout ◽  
Pancreatic Necrosis ◽  
Salvelinus Fontinalis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Salmo Gairdneri ◽  
Blood Components ◽  
Necrosis Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Experimental infection with infectious pancreatic necrosis virus (IPNV) was achieved in yearling rainbow trout (Salmo gairdneri) and 7-mo-old brook trout (Salvelinus fontinalis) by intraperitoneal inoculation and by water contact, respectively. Blood removed periodically from both groups of fish was fractionated and various blood components examined for virus. IPNV was recovered consistently from the plasma and mononuclear-enriched blood fractions of the rainbow trout from 1 to 19 d postinoculation (DPI) and also was detected in the mononuclear fraction on 33 DPI. In the brook trout virus was recovered from 3 to 40 DPI in the plasma and from 7 to 40 DPI in the mononuclear fraction. Thus a viremia appeared to be important in the early stages of experimental IPNV infection and infectious virus was found both free in the plasma and in association with an undetermined cell population present in the mononuclear component of blood.Key words: infectious pancreatic necrosis, blood, fish disease, Salmo gairdneri, Salvelinus fontinalis

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