In vitro selection of Plasmodium falciparum 3D7 for expression of variant surface antigens associated with severe malaria in African children

2003 ◽  
Vol 25 (8-9) ◽  
pp. 421-427 ◽  
Author(s):  
Trine Staalsoe ◽  
Morten A. Nielsen ◽  
Lasse S. Vestergaard ◽  
Anja T. R. Jensen ◽  
Thor G. Theander ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Severe Malaria ◽  
In Vitro Selection ◽  
Surface Antigens ◽  
Variant Surface Antigens ◽  
African Children ◽  
Selection Of

scholarly journals Plasmodium falciparum Parasites Expressing Pregnancy-Specific Variant Surface Antigens Adhere Strongly to the Choriocarcinoma Cell Line BeWo

2006 ◽  
Vol 74 (5) ◽  
pp. 3035-3038 ◽  
Author(s):  
Rikke N. Haase ◽  
Rosette Megnekou ◽  
Maja Lundquist ◽  
Michael F. Ofori ◽  
Lars Hviid ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Cell Line ◽  
Surface Antigens ◽  
Variant Surface Antigens ◽  
Choriocarcinoma Cell Line

ABSTRACT Placenta-sequestering Plasmodium falciparum parasites causing pregnancy-associated malaria express pregnancy-specific variant surface antigens (VSAPAM). We report here that VSAPAM-expressing patient isolates adhere strongly to the choriocarcinoma cell line BeWo and that the BeWo line can be used to efficiently select for VSAPAM expression in vitro.

scholarly journals In vitro selection of Plasmodium falciparum Pfcrt and Pfmdr1 variants by artemisinin

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Muturi J. Njokah ◽  
Joseph N. Kang’ethe ◽  
Johnson Kinyua ◽  
Daniel Kariuki ◽  
Francis T. Kimani
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Selection ◽  
Selection Of

scholarly journals Selective Expression of Variant Surface Antigens Enables Plasmodium falciparum to Evade Immune Clearance in vivo

2020 ◽  
Author(s):  
Marvin Chew ◽  
Weijian Ye ◽  
Radoslaw Igor Omelianczyk ◽  
Charisse Flerida Pasaje ◽  
Regina Hoo ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Immune Evasion ◽  
Immune Escape ◽  
Killer Cell ◽  
Surface Antigens ◽  
Variant Surface Antigens ◽  
Immune Clearance ◽  
Macrophage Phagocytosis

AbstractPlasmodium falciparum has developed extensive mechanisms to evade host immune clearance. Currently, most of our understanding is based on in vitro studies of individual parasite variant surface antigens and how this relates to the processes in vivo is not well-understood. Here, we have used a humanized mouse model to identify parasite factors important for in vivo growth. We show that upregulation of the specific PfEMP1, VAR2CSA and the RIFIN PF3D7_1254800 provides the parasite with protection from macrophage phagocytosis and natural killer cell mediated killing. Taken together, these findings reveal new insights on the molecular and cellular mechanisms that coordinate the immune escape process the parasite utilizes in vivo. As immune evasion may be particularly important during the establishment of the blood stage infection when parasite numbers are still relatively small, identification of specific parasite variant surface antigens provides targets for developing more effective vaccines by targeting parasite immune evasion.

Purification and in Vitro Selection of Rosette-Positive (R+) and Rosette-Negative (R-) Phenotypes of Knob-Positive Plasmodium falciparum Parasites

1992 ◽  
Vol 46 (4) ◽  
pp. 371-381 ◽  
Author(s):  
Shiroma M. Handunnetti ◽  
Russell J. Howard ◽  
Marie-Rose van Schravendijk ◽  
Kei-Ichiro Nakamura ◽  
Aileen D. Gilladoga ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Selection ◽  
Selection Of

In vitro selection of halofantrine resistance in Plasmodium falciparum is not associated with increased expression of Pgh1

1996 ◽  
Vol 83 (1) ◽  
pp. 35-46 ◽  
Author(s):  
Graeme Y Ritchie ◽  
Mathirut Mungthin ◽  
Janet E Green ◽  
Patrick G Bray ◽  
Shaun R Hawley ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
In Vitro Selection ◽  
Selection Of
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