Immunoglobulin Synthesis In Vitro by Cerebrospinal Fluid Cells in Patients with Multiple Sclerosis

1974 ◽  
Vol 3 (6) ◽  
pp. 717-730 ◽  
Author(s):  
M SANDBERG-WOLLHEIM
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Immunoglobulin Synthesis ◽  
Cerebrospinal Fluid Cells

Rapid diagnosis of meningoencephalitis, encephalitis: Immunofluorescent examination of fresh and in vitro cultured cerebrospinal fluid cells

Neurology ◽  
1974 ◽  
Vol 24 (2) ◽  
pp. 143-143 ◽  
Author(s):  
J. LINDEMAN ◽  
W. K. MULLER ◽  
J. VERSTEEG ◽  
G. T. BOTS ◽  
A. C. B. PETERS
Keyword(s):  
Cerebrospinal Fluid ◽  
Rapid Diagnosis ◽  
Cerebrospinal Fluid Cells

Immunophenotyping of Cerebrospinal Fluid Cells in Multiple Sclerosis

2014 ◽  
Vol 71 (7) ◽  
pp. 905 ◽  
Author(s):  
Sascha Alvermann ◽  
Christian Hennig ◽  
Olaf Stüve ◽  
Heinz Wiendl ◽  
Martin Stangel
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Cerebrospinal Fluid Cells

Multiple sclerosis cerebrospinal fluid lymphocytes secretion in vitro: effect on myelinated cultures

1984 ◽  
pp. 246-249
Author(s):  
J. J. Hauw ◽  
O. de Brunier ◽  
J. M. Boutry ◽  
E. Schuller ◽  
E. Roullet ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Vitro Effect

scholarly journals Drug library screen identifies inhibitors of toxic astrogliosis

2020 ◽  
Author(s):  
Ruturaj R Masvekar ◽  
Peter R Kosa ◽  
Christopher Barbour ◽  
Joshua L Milstein ◽  
Bibiana Bielekova
Keyword(s):  
Multiple Sclerosis ◽  
Endoplasmic Reticulum ◽  
Cerebrospinal Fluid ◽  
Calcium Release ◽  
Ryanodine Receptors ◽  
Mammalian Target Of Rapamycin ◽  
Inhibitory Effect ◽  
Multiple Sclerosis Patients

Objective: Multiple sclerosis is a chronic neuroinflammatory disorder, in which activated immune cells directly or indirectly induce demyelination and axonal degradation. Inflammatory stimuli also change the phenotype of astrocytes, making them neurotoxic. The resulting toxic astrocyte phenotype has been observed in animal models of neuroinflammation and in multiple sclerosis lesions. Proteins secreted by toxic astrocytes are elevated in the cerebrospinal fluid of multiple sclerosis patients and reproducibly correlate with the rates of accumulation of neurological disability and brain atrophy. This suggests a pathogenic role for neurotoxic astrocytes in multiple sclerosis. Methods: Here, we applied a commercially available library of small molecules that are either Food and Drug Administration-approved or in clinical development to an in vitro model of toxic astrogliosis to identify drugs and signaling pathways that inhibit inflammatory transformation of astrocytes to a neurotoxic phenotype. Results: Inhibitors of three pathways related to the endoplasmic reticulum stress: 1) proteasome, 2) heat shock protein 90 and 3) mammalian target of rapamycin reproducibly decreased inflammation-induced conversion of astrocytes to toxic phenotype. Dantrolene, an anti-spasticity drug that inhibits calcium release through ryanodine receptors expressed in the endoplasmic reticulum of central nervous system cells, also exerted inhibitory effect at in vivo achievable concentrations. Finally, we established cerebrospinal fluid SERPINA3 as a relevant pharmacodynamic marker for inhibiting toxic astrocytes in clinical trials. Interpretation: Drug library screening provides mechanistic insight into the generation of toxic astrocytes and identifies candidates for immediate proof-of-principle clinical trial(s).

Mitogen and antigen stimulation of multiple sclerosis cerebrospinal fluid lymphocytes in vitro

1983 ◽  
Vol 58 (2) ◽  
pp. 211-221 ◽  
Author(s):  
M Reunanen ◽  
J Ilonen ◽  
T Arnadottir ◽  
A Ahonen ◽  
A Salmi
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
Antigen Stimulation ◽  
Stimulation Of
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