A Deuterium-Hydrogen Exchange Study of Inhibitor-Induced Conformational Changes in Ribonuclease A

1971 ◽  
Vol 21 (3) ◽  
pp. 393-399 ◽  
Author(s):  
Genevieve Nonnenmacher ◽  
Eliane Viala ◽  
Jean Marie Thiery ◽  
Paul Calvet
Keyword(s):  
Conformational Changes ◽  
Hydrogen Exchange ◽  
Ribonuclease A ◽  
Exchange Study

Hydrogen-exchange study of lysozyme conformation changes induced by inhibitor binding

Biochemistry ◽  
1974 ◽  
Vol 13 (16) ◽  
pp. 3273-3277 ◽  
Author(s):  
R. R. Wickett ◽  
G. J. Ide ◽  
A. Rosenberg
Keyword(s):  
Hydrogen Exchange ◽  
Inhibitor Binding ◽  
Conformation Changes ◽  
Exchange Study

scholarly journals Structural lesion to ribonuclease A caused by reductive stress: Assessment by Raman spectroscopy

2008 ◽  
Vol 22 (4) ◽  
pp. 279-286 ◽  
Author(s):  
Armida Torreggiani
Keyword(s):  
Raman Spectroscopy ◽  
Conformational Changes ◽  
Protein Secondary Structure ◽  
Disulfide Bridge ◽  
Acid Sites ◽  
Ribonuclease A ◽  
Rnase A ◽  
Structural Lesion ◽  
Pancreatic Ribonuclease A ◽  
Sulfur Containing

The damages induced by reductive radical stress on bovine pancreatic ribonuclease A (RNAse A) were investigated by Raman spectroscopy. Gamma-irradiation was used to simulate the endogenous formation of reductive species, in particular•H atom that is a simple one-electron equivalent reducing agent. Specific damages occur at sensitive amino acid sites, selectively, rather than indiscriminately, leading to the structure modification of the protein. Sulfur-containing residues (Met and Cys) and aromatic residues are appreciably attacked. In particular, extensive changes in the disulfide bridge conformations are induced as well as conformational changes of the protein secondary structure; a gradual conversion ofα-helical to pleated-sheet geometry was evidenced, indicating a higher ability of reducing radicals in denaturing the protein structure compared with that of oxidizing radical species.

scholarly journals Mapping Temperature-induced Conformational Changes in theEscherichia coliHeat Shock Transcription Factor σ32by Amide Hydrogen Exchange

2003 ◽  
Vol 278 (51) ◽  
pp. 51415-51421 ◽  
Author(s):  
Wolfgang Rist ◽  
Thomas J. D. Jørgensen ◽  
Peter Roepstorff ◽  
Bernd Bukau ◽  
Matthias P. Mayer
Keyword(s):  
Transcription Factor ◽  
Conformational Changes ◽  
Hydrogen Exchange ◽  
Amide Hydrogen ◽  
Amide Hydrogen Exchange

scholarly journals Tracking Local Conformational Changes of Ribonuclease A Using Picosecond Time-Resolved Fluorescence of the Six Tyrosine Residues

2007 ◽  
Vol 92 (12) ◽  
pp. 4401-4414 ◽  
Author(s):  
Melinda Noronha ◽  
João C. Lima ◽  
Emanuele Paci ◽  
Helena Santos ◽  
António L. Maçanita
Keyword(s):  
Conformational Changes ◽  
Ribonuclease A ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Tyrosine Residues
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