scholarly journals Isolation and Characterization of hnRNA-snRNA-Protein Complexes from Morris Hepatoma Cells

2005 ◽  
Vol 128 (1) ◽  
pp. 169-178 ◽  
Author(s):  
Angela PRÜSSE ◽  
Christos LOUIS ◽  
Angel ALONSO ◽  
Constantin E. SEKERIS
Keyword(s):  
Protein Complexes ◽  
Hepatoma Cells ◽  
Isolation And Characterization ◽  
Morris Hepatoma

Arginine synthesis by hepatomas in vitro. II. Isolation and characterization of Morris hepatoma variants unable to convert ornithine to arginine, and modulation of urea-cycle enzymes by dexamethasone and cyclic-AMP

1984 ◽  
Vol 68 (1) ◽  
pp. 305-319
Author(s):  
S.J. Goss
Keyword(s):  
Cyclic Amp ◽  
Liver Cells ◽  
Isolation And Characterization ◽  
Rat Liver Cells ◽  
Morris Hepatoma ◽  
Ornithine Transcarbamoylase ◽  
Cellular Incorporation

‘77orn’, a derivative of the Morris rat hepatoma 7777, stably expresses high levels of ornithine transcarbamoylase (OTC) and carbamoylphosphate synthetase I (CPS-I), and is able to grow indefinitely in ornithine-medium (medium with ornithine in place of arginine). Variants that have lost this ability are isolated from 77orn by a ‘suicide’ selective technique dependent on the cellular incorporation of [3H]ornithine. These variants, which have reduced levels of CPS-I, or of both CPS-I and OTC, are shown to have developed multiple hormonal requirements; their enzyme deficiencies can be reversed by use of an appropriately supplemented medium. In particular, CPS-I is inducible by dexamethasone and dibutyryl-cyclic-AMP in combination. Cholera toxin can be used instead of cyclic-AMP, but then butyrate is additionally required if the induction is to be maintained in the long term. The use of these agents in excess can depress OTC. Several other hepatomas, and alos explanted foetal rat liver cells, have similar requirements for CPS-I expression. It is argued that multiple hormonal requirements for CPS-I production are normal in liver cells in vitro, and that hormone-independent hepatomas should be regarded as abnormal. The implications of this for the somatic cell genetic investigation of differentiation are briefly discussed.

scholarly journals High-throughput Isolation and Characterization of Untagged Membrane Protein Complexes: Outer Membrane Complexes ofDesulfovibrio vulgaris

2012 ◽  
Vol 11 (12) ◽  
pp. 5720-5735 ◽  
Author(s):  
Peter J. Walian ◽  
Simon Allen ◽  
Maxim Shatsky ◽  
Lucy Zeng ◽  
Evelin D. Szakal ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
High Throughput ◽  
Protein Complexes ◽  
Isolation And Characterization ◽  
Membrane Protein Complexes

Isolation and characterization of the plasma membrane from Yoshida hepatoma cells

1975 ◽  
Vol 20 (1) ◽  
pp. 99-110 ◽  
Author(s):  
A. Réthy ◽  
A. Trevisani ◽  
R. Manservigi ◽  
V. Tomasi
Keyword(s):  
Plasma Membrane ◽  
Hepatoma Cells ◽  
Isolation And Characterization

Chromosomale Strukturen von Pseudomonas testosteroni. I. Isolierung und Charakterisierung der chromosomalen Komplexe / Chromosomal Structures of Pseudomonas testosteroni. I. Isolation and Characterization of the Chromosomal Complexes

1976 ◽  
Vol 31 (1-2) ◽  
pp. 91-97 ◽  
Author(s):  
Günter Reimer ◽  
Dusan Drahovsky
Keyword(s):  
Protein Complexes ◽  
E Coli ◽  
Ionic Detergents ◽  
Supercoiled Form ◽  
Isolation And Characterization ◽  
Dnase Digestion ◽  
Dna Packing ◽  
Pronase Treatment ◽  
Pseudomonas Testosteroni

Abstract After lysis of Pseudomonas testosteroni with lysozyme and non-ionic detergents different DNA-protein complexes can be separated in 5 -25% (w/v) neutral sucrose gradient. The protein to DNA ratio of these complexes varies between 0.5-4.5 to 1, whereby the faster sedimenting forms contain more protein than the slower sedimenting ones. Different initial rates of DNase digestion may indicate various degrees of DNA packing in these complexes. The chromosomal complexes of Pseudomonas testosteroni are relatively stable towards pronase. Treatment with RNase or sodium dodecylsulphate is accompanied by a dramatic increase in viscosity and decrease in relative density. It suggests that DNA in these complexes is maintained in its supercoiled form by RNA molecule (s) in a similar way as in isolated chromosome of E. coli.

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