Characterization and Molecular Cloning of Sambucus nigra Agglutinin V (Nigrin b), A Galnac-specific Type-2 Ribosome-Inactivating Protein from the Bark of Elderberry (Sambucus nigra)

1996 ◽  
Vol 237 (2) ◽  
pp. 505-513 ◽  
Author(s):  
Els J. M. Damme ◽  
Annick Barre ◽  
Pierre Rouge ◽  
Fred Leuven ◽  
Willy J. Peumans
Keyword(s):  
Molecular Cloning ◽  
Ribosome Inactivating Protein ◽  
Sambucus Nigra ◽  
Sambucus Nigra Agglutinin

scholarly journals Isolation and Molecular Cloning of a Novel Type 2 Ribosome-inactivating Protein with an Inactive B Chain from Elderberry (Sambucus nigra) Bark

1997 ◽  
Vol 272 (13) ◽  
pp. 8353-8360 ◽  
Author(s):  
Els J. M. Van Damme ◽  
Annick Barre ◽  
Pierre Rougé ◽  
Fred Van Leuven ◽  
Willy J. Peumans
Keyword(s):  
Molecular Cloning ◽  
Ribosome Inactivating Protein ◽  
Sambucus Nigra

scholarly journals Mutational analysis of the carbohydrate-binding activity of the NeuAc(α-2,6)Gal/GalNAc-specific type 2 ribosome-inactivating protein from elderberry (Sambucus nigra) fruits

2002 ◽  
Vol 364 (2) ◽  
pp. 587-592 ◽  
Author(s):  
Ying CHEN ◽  
Pierre ROUGE ◽  
Willy J. PEUMANS ◽  
Els J.M. van DAMME
Keyword(s):  
Mutational Analysis ◽  
Binding Activity ◽  
Site Directed Mutagenesis ◽  
Carbohydrate Binding ◽  
Ribosome Inactivating Protein ◽  
Sambucus Nigra ◽  
Original Activity ◽  
Highly Active ◽  
Critical Residue

Sambucus nigra agglutinin I (SNA-I) is a type 2 ribosome-inactivating protein. Site-directed mutagenesis was used to mimic the conversion of the highly active B-chain of fruit-specific SNA (SNA-If) into the completely inactive B-chain of the closely related and naturally occurring loss-of-activity mutant called S. nigra agglutinin lectin-related protein. In the first mutant SNA-If-M1 the high-affinity site 2 of SNA-If was disrupted by replacing the presumed critical residue Asp231 with Glu231. In the double mutant SNA-If-M2, site 1 of SNA-If-M1 was also disrupted by substituting the presumed critical residue Asn48 with Ser48. The parent type 2 ribosome-inactivating protein and both mutants were expressed in Nicotiana tabacum Samsun NN and the recombinant proteins were purified and analysed. Recombinant SNA-If agglutinated rabbit erythrocytes equally well as SNA-If, but both mutants were completely inactive in this test. Binding assays to immobilized galactose and fetuin revealed that the mutation Asp231→Glu231 reduces the affinity of the B-chain for galactose and fetuin by more than 50%. Furthermore, the introduction of the second mutation Asn48→Ser48 reduces the binding activity to less than 20% of the original activity.

scholarly journals The major elderberry (Sambucus nigra) fruit protein is a lectin derived from a truncated type 2 ribosome-inactivating protein

1997 ◽  
Vol 12 (6) ◽  
pp. 1251-1260 ◽  
Author(s):  
Els J.M. Damme ◽  
Soma Roy ◽  
Annick Barre ◽  
Pierre Rouge ◽  
Fred Leuven ◽  
...  
Keyword(s):  
Ribosome Inactivating Protein ◽  
Sambucus Nigra

Molecular Cloning of a cDNA Encoding Ribosome Inactivating Protein from Amaranthus viridis and Its Expression in E. coli

2000 ◽  
Vol 10 (1) ◽  
pp. 8-12 ◽  
Author(s):  
Suk-Yoon Kwon ◽  
Chung Sun An ◽  
Jang Ryol Liu ◽  
Sang-Soo Kwak ◽  
Haeng-Soon Lee ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Ribosome Inactivating Protein ◽  
E Coli

Effects of Sialic Acid Substitutions on Recognition by Sambucus nigra Agglutinin and Maackia amurensis Hemagglutinin

2002 ◽  
Vol 303 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Els C.M. Brinkman-Van der Linden ◽  
Justin L. Sonnenburg ◽  
Ajit Varki
Keyword(s):  
Sialic Acid ◽  
Sambucus Nigra ◽  
Maackia Amurensis ◽  
Sambucus Nigra Agglutinin
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