Relation of Replication of Tobacco Mosaic Virus and Potato Virus X to Age in Detached Leaves of Nicotiana tabacum

1981 ◽  
Vol 101 (1) ◽  
pp. 16-21
Author(s):  
A. V. Reunov ◽  
G. D. Reunova ◽  
L. A. Lapshina ◽  
V. G. Reifman
1977 ◽  
Vol 90 (4) ◽  
pp. 342-349 ◽  
Author(s):  
A. V. Reunov ◽  
G. D. Reunova ◽  
L. A. Vasilyeva ◽  
V. G. Reifman

2006 ◽  
Vol 87 (4) ◽  
pp. 1005-1012 ◽  
Author(s):  
A. A. Bazzini ◽  
S. Asurmendi ◽  
H. E. Hopp ◽  
R. N. Beachy

Replication of Potato virus X (PVX) was reduced in transgenic protoplasts that accumulated wild-type coat protein (CPWT) of Tobacco mosaic virus (TMV) or a mutant CP, CPT42W, that produced highly ordered states of aggregation, including pseudovirions. This reaction is referred to as heterologous CP-mediated resistance. However, protoplasts expressing a CP mutant that abolished aggregation and did not produce pseudovirions, CPT28W, did not reduce PVX replication. Similarly, in transgenic tobacco plants producing TMV CPWT or CPT42W, there was a delay in local cell-to-cell spread of PVX infection that was not observed in CPT28W plants or in non-transgenic plants. The results suggest that the quaternary structure of the TMV CP regulates the mechanism(s) of heterologous CP-mediated resistance. Similarly, transgenic protoplasts that produced PVX CP conferred transient protection against infection by TMV RNA. Transgenic plants that accumulated PVX CP reduced the cell-to-cell spread of infection and resulted in a delay in systemic infection following inoculation with TMV or TMV RNA. Heterologous CP-mediated resistance was characterized by a brief delay in systemic infection, whilst homologous CP-mediated resistance conferred reduced or no systemic infection.


2010 ◽  
Vol 164 (1-2) ◽  
pp. 7-13 ◽  
Author(s):  
Cristiano Lacorte ◽  
Simone G. Ribeiro ◽  
Dick Lohuis ◽  
Rob Goldbach ◽  
Marcel Prins

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