scholarly journals First Report on the Detection and Molecular Characterization of Bovine Herpesvirus 1 from a Clinical case of Infectious Bovine Rhinotracheitis in Pakistan

2021 ◽  
Vol 41 (01) ◽  
pp. 160-162
Author(s):  
Aayesha Riaz
Keyword(s):  
Molecular Characterization ◽  
Nested Pcr ◽  
Bovine Herpesvirus ◽  
Nasal Discharge ◽  
Infectious Bovine Rhinotracheitis ◽  
Bovine Herpesvirus 1 ◽  
Nasal Swabs ◽  
Herpesvirus 1 ◽  
First Time

Bovine herpesvirus 1 (BHV-1) is a noteworthy reason for many Cattle/Buffalo diseases. Infectious bovine rhinotracheitis (IBR) is one of the diseases which are caused by the BHV-1. In the present study a cow which was suspected of IBR was examined. The animal was suffering from fever and respiratory distress along with rhinitis (red nose), in appetence, and dyspnea. The nasal mucosa and muzzle were distinctly inflamed, with nasal discharge. DNA from blood samples and nasal swabs were subjected to nested PCR using glycoprotein B gene (gB) Primers. The samples were found positive for BHV-1 gB gene. Sequencing and phylogenetic analysis revealed close similarities with other BHV-1 gB gene sequences. The accession numbers assigned to this pioneer sequences in GenBank are MT449510 (BHV-1-Pak 1) and MT449511 (BHV-1-Pak 2). In this study, we reported for the first time the detection of DNA of BHV-1 through nested PCR assay and molecular characterization of BHV-1 gB gene in Pakistan. This study will be useful in further diagnoses of BHV-1 in Pakistan and in development of BHV-1 vaccine to reduce economical losses due to IBR

Molecular Characterization of South American Bovine Herpesvirus-1 Isolates with Monoclonal Antibodies and SDS-PAGE

1993 ◽  
Vol 40 (1-10) ◽  
pp. 125-130 ◽  
Author(s):  
A. Suarez Heinlein ◽  
A. E. Metzler ◽  
R. Weiblen ◽  
P. Berrios ◽  
A. A. Schudel ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Characterization ◽  
Bovine Herpesvirus ◽  
South American ◽  
Bovine Herpesvirus 1 ◽  
Sds Page ◽  
Herpesvirus 1

Evidences of Serological Studies for The Presence of Infectious Bovine Rhinotracheitis IBR, In Albania

1970 ◽  
Vol 3 (1) ◽  
pp. 16-21
Author(s):  
Arta Lugaj ◽  
Liljana Cara ◽  
Marsel Borakaj ◽  
Kristaq Bërxholi
Keyword(s):  
Direct Contact ◽  
Indirect Elisa ◽  
Bovine Herpesvirus ◽  
Infectious Bovine Rhinotracheitis ◽  
Serum Samples ◽  
Chi Square ◽  
Bovine Herpesvirus 1 ◽  
Viral Pathogen ◽  
Herpesvirus 1 ◽  
First Time

Bovine herpesvirus 1 (BHV-1), the causative agent of infectious bovine rhinotracheitis (IBR), is considered to be the most common viral pathogen found in bovine. Virus enters through aerosol route or by direct contact with the nasal secretion in case of respiratory tract infection and by direct contact or by semen containing virus in case of genital infection. For the first time in Albania, this study was conducted to know the status of bovine herpesvirus-1 (BHV-1) antibodies in the bovines of the selected area of Albania. Antibody level was measured using a commercial indirect ELISA. A total of 263 collected serum samples from 7 areas of Albania were subjected to serum neutralization test for detection of BHV-1 antibodies by using of Indirect-ELISA kits. The chi-square test was used for comparison of results between regions and in this study p Values>0.01 was considered statistically no-significant at the 0.01 level. From these results we had an indication about the antibody prevalence of IBR infection respectively, 96% in Terpan-Berat, 52% in Fejza-Has, 50% in Kavajë, 33% in Rrëshen, 14.3% in Guras-Pogradec, 10% in Drenovë-Korçë and 0% in Fier. The prevalence was ranged from 10% to 96% among seropositive herds in this study. In conclusion, results of this study clearly established for the first time that BHV-1 is subclinical prevalent virus in bovine in Albania. Further studies are needed to prevent the spread of this viral infection in Albania.BHV-1,

scholarly journals Neurological disorder in cattle associated with bovine herpesvirus 4

2011 ◽  
Vol 63 (4) ◽  
pp. 828-835 ◽  
Author(s):  
E.A. Costa ◽  
A.C. Vasconcelos ◽  
M.R.Q. Bomfim ◽  
H.B. Amorim ◽  
G.B.L. Lima ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Clinical Course ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus Type ◽  
Neurological Signs ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Herpesvirus 4

A nested PCR assay was used to diagnose bovine encephalitis through herpesviruses including bovine herpesvirus 5 (BHV-5), bovine herpesvirus 1 (BHV-1), Aujeszky's disease virus (SHV-1), and ovine herpesvirus 2 (OHV-2) in 14 fragments of central nervous system (CNS) from cattle that died with neurological signs. In addition, as some samples of bovine herpesvirus type 4 (BHV-4) have been isolated from neural tissue, it was also tested by nested PCR. The cases of encephalitis occurred in isolation at different times of the year and did not present any seasonality. The duration of the clinical course ranged between 1 to 15 days, and in 64.3% of the cases it manifested between 1 to 2 days. The most frequently observed neurological signs were ataxia, recumbency, unsteadiness and inability to stand, opisthotonus, paddling movements, nystagmus and ptyalism. In the nested assay, there was no evidence of: BHV-1, SHV-1 or OHV-2 in the DNA obtained from the CNS in any of the samples. But the presence of BHV-4 was found in all fragments of the CNS in cattle which died presenting neurological signs. Moreover, BHV-5 was found in association with BHV-4 in two of these samples.

Phylogenetic analysis and characterization of bovine herpesvirus-1 in cattle of China, 2016–2019

2020 ◽  
Vol 85 ◽  
pp. 104416 ◽  
Author(s):  
Yulong Zhou ◽  
Xuyang Li ◽  
Yachao Ren ◽  
Xilin Hou ◽  
Yu Liu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1
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