A Comparison of Enzyme-Linked Immunosorbent Assay (ELISA) with Sperm Immobilization Test (SIT) in Detection of Antisperm Antibodies in Sera of Infertile Women

2010 ◽  
Vol 20 (3) ◽  
pp. 305-310 ◽  
Author(s):  
Kuo-Cherng Lin ◽  
Jinu-Huang Su ◽  
Jau-Nan Lee
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antisperm Antibodies ◽  
Infertile Women ◽  
Sperm Immobilization

scholarly journals Human sperm carbohydrate antigens defined by an antisperm human monoclonal antibody derived from an infertile woman bearing antisperm antibodies in her serum.

1988 ◽  
Vol 168 (1) ◽  
pp. 343-356 ◽  
Author(s):  
Y Tsuji ◽  
H Clausen ◽  
E Nudelman ◽  
T Kaizu ◽  
S Hakomori ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Monoclonal Antibody ◽  
Human Sperm ◽  
Infertile Woman ◽  
Antisperm Antibodies ◽  
Infertile Women ◽  
Epitope Structure ◽  
Sperm Immobilization ◽  
Sperm Antigen

The epitope structure of the human sperm antigen reacting with antibodies present in sera of infertile women has been studied using mAb H6-3C4, which produces immobilization of human sperm in the presence of complement. Another antibody, NUH2, which also induces human sperm immobilization, was used to substantiate the presence of a receptor on sperm involved in susceptibility to immobilization. Both antibodies defined type 2 chain polylactosamine structure. H6-3C4 is directed to internally located repetitive N-acetyllactosamine, i.e., sialyl-i, i, or fucosyl-i. NUH2 defines binary alpha 2----3 sialyl type 2 chain, i.e., sialyl-I. Thus, the presence of antibodies in the sera of infertile women directed to sperm lactosaminoglycan or lactosaminolipid could be the basis for infertility in these cases.

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