Three-dimensional visualization of Salmonella attachment to poultry skin using confocal scanning laser microscopy

1996 ◽  
Vol 22 (4) ◽  
pp. 280-282 ◽  
Author(s):  
K.Y. Kim ◽  
J.F. Frank ◽  
S.E. Craven
Keyword(s):  
Three Dimensional ◽  
Scanning Laser ◽  
Confocal Scanning Laser Microscopy ◽  
Laser Microscopy ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

scholarly journals Three-dimensional DNA image cytometry by confocal scanning laser microscopy in thick tissue blocks

Cytometry ◽  
1991 ◽  
Vol 12 (6) ◽  
pp. 511-524 ◽  
Author(s):  
Jean Paul Rigaut ◽  
Jany Vassy ◽  
Paulette Herlin ◽  
Françgoise Duigou ◽  
Eric Masson ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Image Cytometry ◽  
Scanning Laser ◽  
Confocal Scanning Laser Microscopy ◽  
Laser Microscopy ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Laser ◽  
Dna Image Cytometry

Confocal scanning laser microscopy applied to quantify gap junction expression and discriminate different connexin isoforms

1993 ◽  
Vol 51 ◽  
pp. 266-267
Author(s):  
Robert G Gourdie ◽  
Colin R Green ◽  
Robert P Thompson ◽  
Stephen Rothery ◽  
Nicholas S Peters ◽  
...  
Keyword(s):  
Gap Junction ◽  
Three Dimensional ◽  
Scanning Laser ◽  
Confocal Scanning Laser Microscopy ◽  
High Definition ◽  
Laser Microscopy ◽  
Scanning Laser Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Laser ◽  
Gap Junctional

The principles of confocal scanning laser microscopy, and the ability of the technique to provide serial optical sections free from out-of-focus blur for three-dimensional image reconstruction, are now well known to most microscopists. Current studies have entered a new phase — how best to exploit the high definition digital images for maximizing information in specific research applications. Here we report methods for the quantitative analysis of gap junction content per unit volume of tissue and per cell, and for qualitative analysis of the expression of different gap junctional proteins (connexins) in the heart.The first requirements for developing these methods are properly characterized antibodies that, under standardized conditions, give reproducible results in immunofluorescence staining protocols. The strategy as applied to cardiac gap junctions takes account of the unique organization of the junctions into intercalated disks at the end-on abutments between myocytes. Serial optical sectioning techniques are used to prepare projections revealing the entire gap junction population of en face-viewed disks.

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