Cell function studies in long-term bone marrow culture after cell concentration and cryopreservation for autologous transplantation

2009 ◽  
Vol 53 (2) ◽  
pp. 93-99 ◽  
Author(s):  
R. Gilabert ◽  
R. Ayats
Keyword(s):  
Bone Marrow ◽  
Cell Function ◽  
Cell Concentration ◽  
Autologous Transplantation ◽  
Bone Marrow Culture

Cells responsible for restoration of haemopoiesis in long-term murine bone marrow culture

1986 ◽  
Vol 10 (6) ◽  
pp. 659-663 ◽  
Author(s):  
J.L. Chertkov ◽  
Nina J. Drize ◽  
Olga A. Gurevitch ◽  
G.A. Udalov
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Culture ◽  
Murine Bone Marrow

Assessment of bone marrow stem cell reserve and function and stromal cell function in patients with autoimmune cytopenias

Blood ◽  
2000 ◽  
Vol 96 (9) ◽  
pp. 3272-3275 ◽  
Author(s):  
Helen A. Papadaki ◽  
Frances M. Gibson ◽  
Sian Rizzo ◽  
Edward C. Gordon-Smith ◽  
Judith C. W. Marsh
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Stromal Cell ◽  
Cell Function ◽  
Resistant Disease ◽  
Cell Assays ◽  
Factor Data ◽  
Autoimmune Cytopenias ◽  
And Function

Abstract To investigate whether bone marrow (BM) stem cell compartment and/or BM microenvironment are affected by the immune insult in autoimmune cytopenias (AICs), BM stem cell reserve and function and BM stromal function were studied in 15 AIC patients. Stem cells were evaluated by means of flow cytometry, clonogenic progenitor cell assays, long-term BM cultures (LTBMCs), and limiting dilution assay for quantification of long-term–culture initiating cells (LTC-ICs). Stromal cell function was assessed with the use of preformed irradiated LTBMCs from patients and normal controls, recharged with normal CD34+ cells. AIC patients exhibited a high number of CD34+, CD34+/CD38+, and CD34+/CD38− cells; high frequency of granulocyte-macrophage colony forming units in the BM mononuclear cell fraction; high colony recovery in LTBMCs; and normal LTC-IC frequency. Patient BM stromal layers displayed normal hematopoietic-supporting capacity and increased production of granulocyte-colony stimulating factor. Data from this study support the concept that AIC patients with severe, resistant disease might be appropriate candidates for autologous stem cell transplantation.

Can Long-Term Bone Marrow Culture Eliminate Leukemia Cells?

1990 ◽  
Vol 3 (2) ◽  
pp. 87-91 ◽  
Author(s):  
Maria Alessandra Santucci ◽  
Anna Butturini ◽  
Robert Peter Gale
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Culture ◽  
Leukemia Cells

The kinetics of granulopoiesis in long-term mouse bone marrow culture. Part I.

1984 ◽  
Vol 2 (6) ◽  
pp. 394-407 ◽  
Author(s):  
Ursula Reincke ◽  
Markus Loeffler ◽  
H. Erich Wichmann ◽  
Bettina Harrison
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Culture ◽  
Mouse Bone Marrow ◽  
Kinetics Of

The expression of cytokine and cytokine receptor genes in long-term bone marrow culture in congenital and acquired bone marrow hypoplasias

1993 ◽  
Vol 83 (4) ◽  
pp. 560-566 ◽  
Author(s):  
R. Stark ◽  
C. Andre ◽  
D. Thierry ◽  
M. Cherel ◽  
F. Galibert ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Culture ◽  
Cytokine Receptor
Sign in / Sign up

Export Citation Format

Share Document