Diversity of glutelin acidic subunit polypeptides in rice cultivars collected from Northern Vietnam

2014 ◽  
Vol 133 (3) ◽  
pp. 341-347 ◽  
Author(s):  
Tran T. T. Hoai ◽  
Tran D. Suu ◽  
Hikaru Satoh ◽  
Toshihiro Kumamaru

Author(s):  
Jaap Brink ◽  
Wah Chiu

The crotoxin complex is a potent neurotoxin composed of a basic subunit (Mr = 12,000) and an acidic subunit (M = 10,000). The basic subunit possesses phospholipase activity whereas the acidic subunit shows no enzymatic activity at all. The complex's toxocity is expressed both pre- and post-synaptically. The crotoxin complex forms thin crystals suitable for electron crystallography. The crystals diffract up to 0.16 nm in the microscope, whereas images show reflections out to 0.39 nm2. Ultimate goal in this study is to obtain a three-dimensional (3D-) structure map of the protein around 0.3 nm resolution. Use of 100 keV electrons in this is limited; the unit cell's height c of 25.6 nm causes problems associated with multiple scattering, radiation damage, limited depth of field and a more pronounced Ewald sphere curvature. In general, they lead to projections of the unit cell, which at the desired resolution, cannot be interpreted following the weak-phase approximation. Circumventing this problem is possible through the use of 400 keV electrons. Although the overall contrast is lowered due to a smaller scattering cross-section, the signal-to-noise ratio of especially higher order reflections will improve due to a smaller contribution of inelastic scattering. We report here our preliminary results demonstrating the feasability of the data collection procedure at 400 kV.Crystals of crotoxin complex were prepared on carbon-covered holey-carbon films, quench frozen in liquid ethane, inserted into a Gatan 626 holder, transferred into a JEOL 4000EX electron microscope equipped with a pair of anticontaminators operating at −184°C and examined under low-dose conditions. Selected area electron diffraction patterns (EDP's) and images of the crystals were recorded at 400 kV and −167°C with dose levels of 5 and 9.5 electrons/Å, respectively.





Author(s):  
Marc Fredrick Oxenham
Keyword(s):  


2019 ◽  
Vol 26 (6) ◽  
pp. 325-334
Author(s):  
Ivan I. Kropachev ◽  
Nikolai L. Orlov ◽  
Hoa Thi Ninh ◽  
Tao Thien Nguyen

We describe a new species of the Rhacophorus genus, which differs from all species known in Asia by the combination of characters. It strongly differs also from small and middle-sized species of Rhacophorus sensu lato: Rhacophorus calcaneus Smith, 1924, Leptomantis cyanopunctatus (Manthey et Steiof, 1998), Rhacophorus hoabinhensis Nguyen, Pham, Nguyen, Ninh et Ziegler, 2017, Rhacophorus hoanglienensis Orlov, Lathrop, Murphy et Ho, 2001, Zhangixalus jarujini (Matsui et Panha, 2006), Rhacophorus laoshan Mo, Jiang, Xie et Ohler, 2008, Rhacophorus pardalis Günther, 1858, Rhacophorus rhodopus Liu et Hu, 1960, Rhacophorus robertingeri Orlov, Poyarkov, Vassilieva, Ananjeva, Nguyen, Sang, and Geissler, 2012, Leptomantis robinsonii (Boulenger, 1903), Rhacophorus spelaeus Orlov, Gnophanxay, Phimminith, and Phomphoumy, 2010, Rhacophorus translineatus Wu, 1977, Rhacophorus turpes Smith, 1940, Rhacophorus vampyrus Rowley, Le, Thi, Stuart et Hoang, 2010, Rhacophorus viridimaculatus Ostroshabov, Orlov et Nguyen, 2013 by having brown color with two green dorsolateral stripes starting at the groin level and connecting through the distal part of eyelid with green triangle on the head, slender body and head, lower ratio HW/HL 0.86, lower HW/SVL 0.28 and lower ratio HL/SVL 0.32.



2007 ◽  
Vol 24 (0) ◽  
Author(s):  
R.C. Sharma ◽  
N.K. Chaudhary ◽  
B.R. Ojha ◽  
B.K. Joshi ◽  
M.P. Pandey ◽  
...  




2010 ◽  
Vol 19 (1) ◽  
pp. 29-34
Author(s):  
A.V. Gorochov

Prohimerta (Anisotima) laocai sp. nov. from Northern Vietnam and Paraducetia cruciata gialai subsp. nov. from Central Vietnam are described. Corrected are some erroneous data on systematic position of Prohimerta (Anisotima) vieta, P. (Prohimerta) maculosa, and Paraducetia cruciata, as well as those on the type locality of P. maculosa.



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