scholarly journals Cardioprotective effect of hyperthyroidism on the stunned rat heart during ischaemia-reperfusion: energetics and role of mitochondria

2015 ◽  
Vol 100 (6) ◽  
pp. 680-697 ◽  
Author(s):  
María Inés Ragone ◽  
Patricia Bonazzola ◽  
Germán A. Colareda ◽  
Alicia E. Consolini
Keyword(s):  
Rat Heart ◽  
Cardioprotective Effect ◽  
Ischaemia Reperfusion

scholarly journals ROLE OF SDF-1α/CXCR4 AXIS TO THE CARDIOPROTECTIVE EFFECT OF ISCHEMIC POST-CONDITIONING IN ISOLATED RAT HEART

2013 ◽  
Vol 61 (10) ◽  
pp. E217
Author(s):  
Jeong-Su Kim ◽  
Ju-Hyun Park ◽  
Kook-Jin Chun ◽  
Young-Ho Jang ◽  
June-Hong Kim ◽  
...  
Keyword(s):  
Rat Heart ◽  
Isolated Rat Heart ◽  
Cardioprotective Effect ◽  
Cxcr4 Axis ◽  
Isolated Rat

scholarly journals Pertussis toxin abolishes the cardioprotective effect of ischemic preconditioning in intact rat heart

1998 ◽  
Vol 275 (2) ◽  
pp. H495-H500 ◽  
Author(s):  
Jo El J. Schultz ◽  
Anna K. Hsu ◽  
Joseph T. Barbieri ◽  
Pin-Lan Li ◽  
Garrett J. Gross
Keyword(s):  
Pertussis Toxin ◽  
Ischemic Preconditioning ◽  
Rat Heart ◽  
Cardioprotective Effect ◽  
Control Group ◽  
Area At Risk ◽  
Small Subpopulation

It has been previously demonstrated that Gi/o proteins are involved in ischemic preconditioning (IPC) in rabbits and dogs; however, there has been controversy as to the role of Gi/o proteins in IPC in in vivo rat infarct models. Therefore, the role of G proteins in the cardioprotective effect of IPC in a rat infarct model was reevaluated. Cardioprotection as indicated by infarct size (IS) as a percentage of the area at risk (IS/AAR) was determined by triphenyltetrazolium stain. The control group, which was subjected to 30 min of occlusion (Occ) and 2 h of reperfusion (Rep), had an IS/AAR of 46 ± 6%. A single 5-min Occ followed by 10 min of Rep (1× PC) as well as three 5-min Occ periods interspersed with 5 min of Rep (3× PC) markedly reduced IS/AAR (6 ± 1 and 8 ± 1%, respectively). Pretreatment with pertussis toxin (10 μg/kg ip) for 48 h before 1× PC or 3× PC completely abolished their cardioprotective effects (46 ± 5 and 38 ± 4%, respectively). Pertussis toxin had no effect on IS/AAR and did not inactivate Gi/o proteins as assessed by an in vitro ADP-ribosylation assay of heart homogenates. These results demonstrate that the cardioprotective effect of IPC is mediated by a small subpopulation of Gi/o proteins in the intact rat heart.

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