Single particle tracking through highly scattering media with multiplexed two-photon excitation

2015 ◽  
Author(s):  
Evan Perillo ◽  
Yen-Liang Liu ◽  
Cong Liu ◽  
Hsin-Chih Yeh ◽  
Andrew K. Dunn
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Scattering Media ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals Improving z-tracking accuracy in the two-photon single-particle tracking microscope

2015 ◽  
Vol 107 (15) ◽  
pp. 153701 ◽  
Author(s):  
C. Liu ◽  
Y.-L. Liu ◽  
E. P. Perillo ◽  
N. Jiang ◽  
A. K. Dunn ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Tracking Accuracy ◽  
Two Photon

Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

2004 ◽  
Vol 65 (4-5) ◽  
pp. 169-179 ◽  
Author(s):  
H.E. Grecco ◽  
K.A. Lidke ◽  
R. Heintzmann ◽  
D.S. Lidke ◽  
C. Spagnuolo ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Single Particle ◽  
Photophysical Properties ◽  
Live Cells ◽  
Two Photon ◽  
Photon Excitation ◽  
Spectral Conversion ◽  
Two Photon Excitation

Spatially localized ballistic two-photon excitation in scattering media

1998 ◽  
Vol 4 (5) ◽  
pp. 303-310 ◽  
Author(s):  
Henryk Szmacinski ◽  
Ignacy Gryczynski ◽  
Joseph R. Lakowicz
Keyword(s):  
Scattering Media ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals Two-photon excitation in scattering media by spatiotemporally shaped beams and their application in optogenetic stimulation

2013 ◽  
Vol 4 (12) ◽  
pp. 2869 ◽  
Author(s):  
Aurélien Bègue ◽  
Eirini Papagiakoumou ◽  
Ben Leshem ◽  
Rossella Conti ◽  
Leona Enke ◽  
...  
Keyword(s):  
Scattering Media ◽  
Two Photon ◽  
Optogenetic Stimulation ◽  
Photon Excitation ◽  
Two Photon Excitation

Spatially distributed two-photon excitation fluorescence in scattering media: Experiments and time-resolved Monte Carlo simulations

2007 ◽  
Vol 272 (1) ◽  
pp. 269-278 ◽  
Author(s):  
A. Leray ◽  
C. Odin ◽  
E. Huguet ◽  
F. Amblard ◽  
Y. Le Grand
Keyword(s):  
Monte Carlo ◽  
Monte Carlo Simulations ◽  
Scattering Media ◽  
Time Resolved ◽  
Two Photon ◽  
Photon Excitation ◽  
Spatially Distributed ◽  
Two Photon Excitation

scholarly journals Improving Z-Tracking Accuracy in Two-Photon Single-Particle Tracking Microscope

2016 ◽  
Vol 110 (3) ◽  
pp. 633a
Author(s):  
Cong Liu ◽  
Evan Perillo ◽  
Yen-Liang Liu ◽  
Ajay Rastog ◽  
Andrew Dunn ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Tracking Accuracy ◽  
Two Photon

Two-Photon Microscopy of Single Molecules

2000 ◽  
Vol 6 (S2) ◽  
pp. 804-805
Author(s):  
R. Yuste ◽  
A. Majewska ◽  
K. Holthoff ◽  
K. Holthoff
Keyword(s):  
Dendritic Spines ◽  
Pyramidal Neurons ◽  
Calcium Influx ◽  
Pyramidal Cells ◽  
Scattering Media ◽  
Decay Kinetics ◽  
Two Photon ◽  
Photon Excitation ◽  
Cortical Pyramidal Neurons ◽  
Two Photon Excitation

Two-photon excitation has enabled investigators to image living cells in highly scattering media like the central nervous system (1). We have used a custom-built two-photon microscope to image dendritic spines from living cortical pyramidal neurons. Pyramidal cells form the majority of the neuron in the mammalian cortex and they receive practically all their synaptic contacts through dendritic spines. Dendritic spines are small (<1 μm diameter) appendages that have been practically inaccessible to physiological measurements until the application of two-photon excitation to their study (2). We have concentrated in two questions:A- Calcium compartmentalization of spines: Mechanisms of calcium decay kinetics.Dendritic spines can compartmentalize calcium (2). Although the mechanisms of calcium influx into spines have been explored (3), it is unknown what determines the calcium decay kinetics in spines. We investigate calcium dynamics in spines from rat CA1 pyramidal neurons in slices.

scholarly journals Two-photon single particle tracking in 3D

1997 ◽  
Author(s):  
Peter T. C. So ◽  
Timothy Ragan ◽  
Enrico Gratton ◽  
Jenny Carerro ◽  
Edward Voss
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Two Photon

NIR-emitting molecular-based nanoparticles as new two-photon absorbing nanotools for single particle tracking

2015 ◽  
Author(s):  
J. Daniel ◽  
A. G. Godin ◽  
G. Clermont ◽  
B. Lounis ◽  
L. Cognet ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Two Photon

Two-photon excitation microscopy in cellular biophysics

1995 ◽  
Vol 53 ◽  
pp. 62-63
Author(s):  
David W. Piston ◽  
Brian D. Bennett ◽  
Robert G. Summers
Keyword(s):  
Confocal Microscopy ◽  
Laser Beam ◽  
Duty Cycle ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10-5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

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