Multimodal polarization-resolved/fluorescence optical scanning microscopy for chromatin organization imaging

2021 ◽  
Author(s):  
Ali Mohebi ◽  
Aymeric Le Gratiet ◽  
Fabio Callegari ◽  
Paolo Bianchini ◽  
Alberto Diaspro
Keyword(s):  
Chromatin Organization ◽  
Scanning Microscopy ◽  
Optical Scanning

scholarly journals Fast spectrally encoded Mueller optical scanning microscopy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sylvain Rivet ◽  
Matthieu Dubreuil ◽  
Adrian Bradu ◽  
Yann Le Grand
Keyword(s):  
Scanning Microscopy ◽  
Optical Scanning ◽  
Spectrally Encoded

Near‐field optical‐scanning microscopy

1986 ◽  
Vol 59 (10) ◽  
pp. 3318-3327 ◽  
Author(s):  
U. Dürig ◽  
D. W. Pohl ◽  
F. Rohner
Keyword(s):  
Near Field ◽  
Scanning Microscopy ◽  
Optical Scanning

Near‐field optical scanning microscopy in reflection

1988 ◽  
Vol 52 (4) ◽  
pp. 249-251 ◽  
Author(s):  
U. Ch. Fischer ◽  
U. T. Dürig ◽  
D. W. Pohl
Keyword(s):  
Near Field ◽  
Scanning Microscopy ◽  
Optical Scanning

Biological Relevancy of Quantitative Analysis of Adherence Receptors on Polymorphonuclear.Neutrophil by Flow Cytometry and Optical Scanning Microscopy..

1999 ◽  
Vol 5 (S2) ◽  
pp. 1122-1123
Author(s):  
Dominique Dumas ◽  
Véronique Latger ◽  
Jean F. Stoltz
Keyword(s):  
Flow Cytometry ◽  
Molecular Conformation ◽  
Polymorphonuclear Neutrophils ◽  
Scanning Microscopy ◽  
Optical Scanning ◽  
Surface Molecules ◽  
Activation Level ◽  
Cellular Population ◽  
Necrosis Factor

Leukocytes adherence receptors are the basis of cells interactions1. Flow cytometry (FCM) makes it possible to assess a characterization of the activation level of a given cellular population by receptor quantifying2. That technique, however does not integrate at the same time, all others factors of leucocyte adhesive phenotype regulation, as spatial distribution and molecular conformation. Our study consisted in exploring the main adhesion receptors (CD62L, CDllb/CD18) on the surface of Polymorphonuclear Neutrophils (PMN) that were prepared under identical conditions and simultaneously analyzed by FCM and Conventional Optical Scanning Microscopy/Deconvolution3 (COSM). Methods: PMN cells from whole blood were obtained using Polymorphprep™. Isolated PMN (105 cells /ml) were incubated with Tumor Necrosis Factor (TNFα, 100 UI/ml) at 37°C for one hour. Quantification of cell surface molecules was assessed according to the quantitative indirect immunofluorescence3 method using QIFIKIT® (Dako, France).

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