In vitro embryogenesis

Science ◽  
2017 ◽  
Vol 356 (6334) ◽  
pp. 149.12-151
Author(s):  
Beverly A. Purnell
Keyword(s):  
In Vitro Embryogenesis

scholarly journals MICROPROPAGATION OF PHALAENOPSIS SPP. BY SOMATIC EMBRYOGENESIS TECHNIQUE

2018 ◽  
Vol 6 ◽  
pp. 1185-1191
Author(s):  
Minh Van Tran
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Cell ◽  
Embryonic Cell ◽  
Cell Suspensions ◽  
Ms Medium ◽  
Callus Initiation ◽  
Set Up ◽  
Planting Materials ◽  
In Vitro Embryogenesis

Phalaenopsis spp. was regularly produced through micropropagation by protocorm like bodies (PLBs); micropropagation takes a lot of labor, and has high cost of seedlings, energy and material. The purpose of this paper was to study the new technique of using in vitro embryogenesis culturing for microprogation. The method involved using protocorm like bodies as planting materials. PLBs were cut into slices and placed on the medium for callus initiation. The callus was initiated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l) or 2,4D (1 mg/l) and was proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l). Somatic cell suspensions were initiated and proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (0.5, 1 mg/l). Somatic cell suspensions were differentiated to embryonic cell suspensions on the MS medium supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Embryonic cell suspensions were plated and regenerated on the medium: 1/2MS supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Micropropagation of Phalaenopsis sp. via the embryogenesis technique was set up to produce 5,800 plantlets per one liter of somatic embryogenesis suspension.

In Vitro Embryogenesis in Plants

1996 ◽  
Vol 38 (4) ◽  
pp. 616-616
Author(s):  
J. Opatrna
Keyword(s):  
In Vitro Embryogenesis

scholarly journals Study of Oil Palm (Elaeis guineensis Jacq) In Vitro Embryogenesis using Young Leaves Explants

2015 ◽  
Vol 2 (2) ◽  
pp. 5-9
Author(s):  
Ni Made Armini Wiendi ◽  
Mondjeli Constantin ◽  
Ade Wachyar
Keyword(s):  
Oil Palm ◽  
Embryogenic Callus ◽  
Elaeis Guineensis ◽  
Leaf Explants ◽  
Embryogenic Calli ◽  
Auxin Concentration ◽  
Young Leaves ◽  
Embryogenic Callus Induction ◽  
In Vitro Embryogenesis

This study reported in vitro embryogenesis of oil palm using young leaves as explants. Explants were grown in solid modified MS or Eeuwens medium containing different concentrations of NAA and 2,4-D, i.e. media C1, C2, C3, C4 and C5, M1, M2, M3 and M4, to induce embryogenic calli. Compact and pearly-white, globular calli were obtained from the youngest leaf explants 28 weeks after culture.C1 media (MS medium + 107.41 µM of NAA + 100 mg.L-1 of asparagine + 100 mg.L of glutamine-1) produced the highest percentage of calli formation (30.56%), whereas C4 media (C1 supplemented with 67.86 µM of 2,-D ) was the optimal media for embryogenic callus induction. Direct embryoids were obtained from slightly older leaf explants on the C3 media containing NAA after 36 weeks of culture. However, four subcultures using the same medium with gradual reduction of auxin concentration were not successful to develop embryogenic callus and embryoid cells during the course of this study.  

In vitro embryogenesis of Macrobrachium rosenbergii larvae following in vivo fertilization

Aquaculture ◽  
1996 ◽  
Vol 147 (3-4) ◽  
pp. 169-175 ◽  
Author(s):  
Thomas Caceci ◽  
Catherine B. Carlson ◽  
Thomas E. Toth ◽  
Stephen A. Smith
Keyword(s):  
Macrobrachium Rosenbergii ◽  
In Vitro Embryogenesis

IN VITRO EMBRYOGENESIS: AN EXPERIMENTAL MODEL FOR THE UNDERSTANDING OF REPRODUCTIVE PHYSIOLOGY AND DEVELOPMENT IN MAMMALS

1974 ◽  
Vol 16 (1) ◽  
pp. 11-30
Author(s):  
Anil B. Mukherjee
Keyword(s):  
Gene Action ◽  
Reproductive Physiology ◽  
Nuclear Transplantation ◽  
In Vitro Technique ◽  
Potential Applications ◽  
Oocyte Cytoplasm ◽  
In Vitro Embryogenesis ◽  
Spermatozoa Maturation

A complete in vitro technique has been developed in the mouse for capacitation of epididymal spermatozoa, maturation of ovarian oocytes and fertilization of such gemetes to give rise to viable embryos, which, when transplanted to proper recipients result in normal, fertile progeny. This technique, although it requires perfection, has many future possibilities and potential applications as a research tool in solving problems of mammalian reproductive physiology and developmental biology. It may be useful in the investigation of (a) gene action in the X chromosome and initiation of X-inactivation during embryogenesis; (b) nuclear transplantation and the ontogenetic commitment of oocyte cytoplasm; (c) the role of drugs, mutagens and carcinogens in the fetal environment; (d) genetic engineering; eg. enzyme induction and (e) many problems involving fertility and sterility.

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