Transcriptional changes of antioxidant responses, hormone signalling and developmental processes evoked by the Brassica napus SHOOTMERISTEMLESS during in vitro embryogenesis

2012 ◽  
Vol 58 ◽  
pp. 297-311 ◽  
Author(s):  
Mohamed Elhiti ◽  
Cunchun Yang ◽  
Mark F. Belmonte ◽  
Robert H. Gulden ◽  
Claudio Stasolla
Keyword(s):  
Brassica Napus ◽  
Developmental Processes ◽  
Antioxidant Responses ◽  
Transcriptional Changes ◽  
In Vitro Embryogenesis ◽  
Hormone Signalling

scholarly journals Plasticity in Cell Division Patterns and Auxin Transport Dependency during in Vitro Embryogenesis in Brassica napus

2014 ◽  
Vol 26 (6) ◽  
pp. 2568-2581 ◽  
Author(s):  
Mercedes Soriano ◽  
Hui Li ◽  
Cédric Jacquard ◽  
Gerco C. Angenent ◽  
Joan Krochko ◽  
...  
Keyword(s):  
Cell Division ◽  
Brassica Napus ◽  
Auxin Transport ◽  
In Vitro Embryogenesis

scholarly journals Analysis of Transcriptional Changes in Different Brassica napus Synthetic Allopolyploids

Genes ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 82
Author(s):  
Yunxiao Wei ◽  
Guoliang Li ◽  
Shujiang Zhang ◽  
Shifan Zhang ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Brassica Napus ◽  
Differentially Expressed Genes ◽  
Expression Patterns ◽  
Female Parent ◽  
Enrichment Analysis ◽  
Differentially Expressed ◽  
Transcriptional Changes ◽  
Aa Genome ◽  
High Degree

Allopolyploidy is an evolutionary and mechanistically intriguing process involving the reconciliation of two or more sets of diverged genomes and regulatory interactions, resulting in new phenotypes. In this study, we explored the gene expression patterns of eight F2 synthetic Brassica napus using RNA sequencing. We found that B. napus allopolyploid formation was accompanied by extensive changes in gene expression. A comparison between F2 and the parent shows a certain proportion of differentially expressed genes (DEG) and activation\silent gene, and the two genomes (female parent (AA)\male parent (CC) genomes) showed significant differences in response to whole-genome duplication (WGD); non-additively expressed genes represented a small portion, while Gene Ontology (GO) enrichment analysis showed that it played an important role in responding to WGD. Besides, genome-wide expression level dominance (ELD) was biased toward the AA genome, and the parental expression pattern of most genes showed a high degree of conservation. Moreover, gene expression showed differences among eight individuals and was consistent with the results of a cluster analysis of traits. Furthermore, the differential expression of waxy synthetic pathways and flowering pathway genes could explain the performance of traits. Collectively, gene expression of the newly formed allopolyploid changed dramatically, and this was different among the selfing offspring, which could be a prominent cause of the trait separation. Our data provide novel insights into the relationship between the expression of differentially expressed genes and trait segregation and provide clues into the evolution of allopolyploids.

scholarly journals EST-based in silico identification and in vitro test of antimicrobial peptides in Brassica napus

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Tao Ke ◽  
Huihui Cao ◽  
Junyan Huang ◽  
Fan Hu ◽  
Jin Huang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Antimicrobial Peptides ◽  
In Silico ◽  
In Vitro Test ◽  
Vitro Test ◽  
In Silico Identification

I.—On the Developmental Processes in Mammary Glands and other Epidermal Structures

1952 ◽  
Vol 62 (1) ◽  
pp. 1-31 ◽  
Author(s):  
B. I. Balinsky
Keyword(s):  
Alkaline Phosphatase ◽  
Nucleic Acid ◽  
Early Development ◽  
Mammary Glands ◽  
Mitotic Rate ◽  
Hair Follicles ◽  
Developmental Processes ◽  
Histochemical Methods

Synopsis:The early development of the mammary glands and of hair follicles in the mouse and the rabbit has been investigated by means of mitotic counts, by histochemical methods and by cultivation in vitro. In the first stage of the formation of both types of rudiment a thickening of the epidermis appears as a consequence of an aggregation of cells, not of a local elevation of the mitotic rate. During this stage alkaline phosphatase is absent from the cells and the content of ribose-nucleic acid reduced. Both these substances appear in higher concentrations later, and their relation to processes of growth and differentiation is discussed. Essentially similar phenomena are found in rudiments cultivated in vitro, which, however, frequently suffer a degeneration by a process of keratinisation.

scholarly journals In vitro germination and biochemical profiling of Brassica napus in response to biosynthesised zinc nanoparticles

2018 ◽  
Vol 13 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Sohail ◽  
Umme Amara ◽  
Salma Shad ◽  
Noshin Ilyas ◽  
Abdul Manaf ◽  
...  
Keyword(s):  
Brassica Napus ◽  
In Vitro Germination ◽  
Zinc Nanoparticles

scholarly journals Transcriptome Analyses of In Vitro Exercise Models by Clenbuterol Supplementation or Electrical Pulse Stimulation

2021 ◽  
Vol 11 (21) ◽  
pp. 10436
Author(s):  
Taku Fukushima ◽  
Miho Takata ◽  
Ayano Kato ◽  
Takayuki Uchida ◽  
Takeshi Nikawa ◽  
...  
Keyword(s):  
Gene Ontology ◽  
Electrical Pulse ◽  
C2c12 Myotubes ◽  
Rna Seq ◽  
Gene Expressions ◽  
Beneficial Effects ◽  
Electrical Pulse Stimulation ◽  
Transcriptional Changes ◽  
Pulse Stimulation

Exercise has beneficial effects on human health and is affected by two different pathways; motoneuron and endocrine. For the advancement of exercise research, in vitro exercise models are essential. We established two in vitro exercise models using C2C12 myotubes; EPS (electrical pulse stimulation) for a motoneuron model and clenbuterol, a specific β2 adrenergic receptor agonist, treatment for an endocrine model. For clenbuterol treatment, we found that Ppargc1a was induced only in low glucose media (1 mg/mL) using a 1-h treatment of 30 ng/mL clenbuterol. Global transcriptional changes of clenbuterol treatment were analyzed by RNA-seq and gene ontology analyses and indicated that mitogenesis and the PI3K-Akt pathway were enhanced, which is consistent with the effects of exercise. Cxcl1 and Cxcl5 were identified as candidate myokines induced by adrenaline. As for the EPS model, we compared 1 Hz of 1-pulse EPS and 1 Hz of 10-pulse EPS for 24 h and determined Myh gene expressions. Ten-pulse EPS induced higher Myh2 and Myh7 expression. Global transcriptional changes of 10-pulse EPS were also analyzed using RNA-seq, and gene ontology analyses indicated that CaMK signaling and hypertrophy pathways were enhanced, which is also consistent with the effects of exercise. In this paper, we provided two transcriptome results of in vitro exercise models and these databases will contribute to advances in exercise research.

scholarly journals MICROPROPAGATION OF PHALAENOPSIS SPP. BY SOMATIC EMBRYOGENESIS TECHNIQUE

2018 ◽  
Vol 6 ◽  
pp. 1185-1191
Author(s):  
Minh Van Tran
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Cell ◽  
Embryonic Cell ◽  
Cell Suspensions ◽  
Ms Medium ◽  
Callus Initiation ◽  
Set Up ◽  
Planting Materials ◽  
In Vitro Embryogenesis

Phalaenopsis spp. was regularly produced through micropropagation by protocorm like bodies (PLBs); micropropagation takes a lot of labor, and has high cost of seedlings, energy and material. The purpose of this paper was to study the new technique of using in vitro embryogenesis culturing for microprogation. The method involved using protocorm like bodies as planting materials. PLBs were cut into slices and placed on the medium for callus initiation. The callus was initiated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l) or 2,4D (1 mg/l) and was proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l). Somatic cell suspensions were initiated and proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (0.5, 1 mg/l). Somatic cell suspensions were differentiated to embryonic cell suspensions on the MS medium supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Embryonic cell suspensions were plated and regenerated on the medium: 1/2MS supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Micropropagation of Phalaenopsis sp. via the embryogenesis technique was set up to produce 5,800 plantlets per one liter of somatic embryogenesis suspension.

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