Effects of Specific Zidovudine Resistance Mutations and Substrate Structure on Nucleotide-Dependent Primer Unblocking by Human Immunodeficiency Virus Type 1 Reverse Transcriptase
2002 ◽
Vol 46
(5)
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pp. 1540-1545
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Keyword(s):
ABSTRACT Nucleotide-dependent unblocking of chain-terminated DNA by human immunodeficiency virus type 1 reverse transcriptase (RT) is enhanced by the presence of mutations associated with 3′-azido-3′-deoxythymidine (AZT) resistance. The increase in unblocking activity was greater for mutant combinations associated with higher levels of in vivo AZT resistance. The difference between mutant and wild-type activity was further enhanced by introduction of a methyl group into the nucleotide substrate and was decreased for a nonaromatic substrate, suggesting that π-π interactions between RT and an aromatic structure may be facilitated by these mutations.
2003 ◽
Vol 77
(11)
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pp. 6127-6137
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1994 ◽
Vol 75
(5)
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pp. 951-957
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2001 ◽
Vol 75
(10)
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pp. 4832-4842
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2002 ◽
Vol 76
(7)
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pp. 3248-3256
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2009 ◽
Vol 53
(11)
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pp. 4667-4672
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2001 ◽
Vol 75
(15)
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pp. 7202-7205
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2000 ◽
Vol 74
(2)
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pp. 1023-1028
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2004 ◽
Vol 48
(11)
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pp. 4493-4494
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1999 ◽
Vol 73
(4)
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pp. 3455-3459
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