scholarly journals Identification of Critical Members in a Sulfidogenic Benzene-Degrading Consortium by DNA Stable Isotope Probing

2008 ◽  
Vol 74 (20) ◽  
pp. 6476-6480 ◽  
Author(s):  
A. R. Oka ◽  
C. D. Phelps ◽  
L. M. McGuinness ◽  
A. Mumford ◽  
L. Y. Young ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Stable Isotope Probing ◽  
Polymorphism Analysis ◽  
Benzene Degradation ◽  
Anaerobic Benzene Degradation ◽  
Restriction Fragment Length

ABSTRACT Stable isotope probing (SIP) was used to identify the active members in a benzene-degrading sulfidogenic consortium. SIP-terminal restriction fragment length polymorphism analysis indicated that a 270-bp peak incorporated the majority of the 13C label and is a sequence closely related to that of clone SB-21 (GenBank accession no. AF029045). This target may be an important biomarker for anaerobic benzene degradation in the field.

scholarly journals Detection of 2,4,6-Trinitrotoluene-Utilizing Anaerobic Bacteria by 15N and 13C Incorporation

2010 ◽  
Vol 76 (5) ◽  
pp. 1695-1698 ◽  
Author(s):  
Erin M. Gallagher ◽  
Lily Y. Young ◽  
Lora M. McGuinness ◽  
Lee J. Kerkhof
Keyword(s):  
Stable Isotope ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Anaerobic Bacteria ◽  
Stable Isotope Probing ◽  
Restriction Fragment Length ◽  
Harbor Sediments

ABSTRACT 2,4,6-Trinitrotoluene (15N or 13C labeled) was added to Norfolk Harbor sediments to test whether anaerobic bacteria use TNT for growth. Stable-isotope probing (SIP)-terminal restriction fragment length polymorphism (TRFLP) detected peaks in the [15N]TNT cultures (60, 163, and 168 bp). The 60-bp peak was also present in the [13C]TNT cultures and was related to Lysobacter taiwanensis.

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