scholarly journals Feasibility of Detecting Human Immunodeficiency Virus Type 1 Drug Resistance in DNA Extracted from Whole Blood or Dried Blood Spots

2007 ◽  
Vol 45 (10) ◽  
pp. 3342-3351 ◽  
Author(s):  
K. Steegen ◽  
S. Luchters ◽  
E. Demecheleer ◽  
K. Dauwe ◽  
K. Mandaliya ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Drug Resistance ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Whole Blood ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Immunodeficiency Virus

scholarly journals Evaluation of the human immunodeficiency virus type 1 and 2 antibodies detection in dried whole blood spots (dbs) samples

2008 ◽  
Vol 50 (3) ◽  
pp. 151-156 ◽  
Author(s):  
Andréa Cauduro de Castro ◽  
Luiz Gustavo dos Anjos Borges ◽  
Ricardo da Silva de Souza ◽  
Melina Grudzinski ◽  
Pedro Alves D'Azevedo
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Whole Blood ◽  
Confirmatory Test ◽  
Blood Spots ◽  
Immunodeficiency Virus ◽  
Blood Spot ◽  
Antibodies Detection

Human Immunodeficiency Vírus Type 1 and 2 antibodies detection was performed in 457 dried whole blood spots samples (S&S 903). Q-Preven HIV 1+2 was the screening test used. The results were compared with the gold standard serum tests by ELISA (Cobas Core e Axsym HIV1/2 gO) and imunofluorescence was the definitive confirmatory test. The samples were obtained from the Hospital Nossa Senhora da Conceição in Porto Alegre, RS - Brazil, through whole blood transfer to filter paper card and sent to Caxias do Sul, RS - Brazil where the tests were performed. The dried whole blood spot stability was evaluated with two different panels. The first one was composed of five negative and five positive samples stored at room temperature, 4 ºC, -20 ºC and -70 ºC, while the second was composed of two negative and three positive samples stored at 37 ºC (humidity <50%). Each sample was screened every week for six weeks. These measurement results didn't show variation during the study period. The detected sensibility was 100%, specificity was 99.6%, the positive predictive value was 99.5% and negative predictive values were 100%. The results demonstrated high performance characteristics, opening a new perspective of dried whole blood spot utilization in HIV screening diagnosis.

scholarly journals Evaluation of Dried Whole Blood Spots Obtained by Heel or Finger Stick as an Alternative to Venous Blood for Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Vertically Exposed Infants in the Routine Diagnostic Laboratory

2006 ◽  
Vol 14 (2) ◽  
pp. 201-203 ◽  
Author(s):  
Janet C. Patton ◽  
Eveline Akkers ◽  
Ashraf H. Coovadia ◽  
Tammy M. Meyers ◽  
Wendy S. Stevens ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Diagnostic Laboratory ◽  
Blood Spots ◽  
Immunodeficiency Virus ◽  
Finger Stick

ABSTRACT The diagnostic accuracy of the Roche Amplicor human immunodeficiency virus type 1 DNA PCR assay (version 1.5) on DNA extracted from pediatric heel prick dried blood spots using Roche MagNA Pure nucleic acid purification technology was evaluated. The methodologies transfer successfully from the labor-intensive research laboratory to the high-throughput automated routine laboratory.

Sign in / Sign up

Export Citation Format

Share Document