scholarly journals Evaluation of Matrix-Assisted Laser Desorption Ionization−Time of Flight Mass Spectrometry for Identification of Mycobacterium abscessus Subspecies According to Whole-Genome Sequencing

2016 ◽  
Vol 54 (12) ◽  
pp. 2982-2989 ◽  
Author(s):  
Liulin Luo ◽  
Weijia Liu ◽  
Bing Li ◽  
Mengling Li ◽  
Dongdong Huang ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Mycobacterium Abscessus ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Vitek Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

This study was undertaken to evaluate the utility of matrix-assisted laser desorption ionization–time of flight mass spectrometry with the Vitek MS Plus system for identifying Mycobacterium abscessus subspecies in order to facilitate more rapid and appropriate therapy. A total of 175 clinical M. abscessus strains were identified by whole-genome sequencing analysis: 139 Mycobacterium abscessus subsp. abscessus and 36 Mycobacterium abscessus subsp. massiliense . The research-use-only (RUO) Saramis Knowledge Base database v.4.12 was modified accordingly by adding 40 M. abscessus subsp. abscessus and 19 M. abscessus subsp. massiliense reference spectra to construct subspecies SuperSpectra. A blind test, used to validate the remaining 116 isolates, yielded 99.1% ( n = 115) reliability and only 0.9% ( n = 1) error for subspecies identification. Among the two subspecies SuperSpectra, two specific peaks were found for M. abscessus subsp. abscessus and four specific peaks were found for M. abscessus subsp. massiliense . Our study is the first to report differential peaks 3,354.4 m / z and 6,711.1 m / z , which were specific for M. abscessus subsp. massiliense . Our research demonstrates the capacity of the Vitek MS RUO Saramis Knowledge Base database to identify M. abscessus at the subspecies level. Moreover, it validates the potential ease and accuracy with which it can be incorporated into the IVD system for the identification of M. abscessus subspecies.

scholarly journals A Comprehensive Evaluation of the Bruker Biotyper MS and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Yeasts, Part of the National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) Study, 2012 to 2013

2016 ◽  
Vol 54 (5) ◽  
pp. 1376-1380 ◽  
Author(s):  
He Wang ◽  
Yan-Yan Fan ◽  
Timothy Kudinha ◽  
Zhi-Peng Xu ◽  
Meng Xiao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Vitek Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Among the 2,683 yeast isolates representing 41 different species (25CandidaandCandida-related species and 16 non-Candidayeast species) collected in the National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) program (2012 to 2013), the Bruker Biotyper MS matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) system exhibited significantly higher accuracy rates than the Vitek MS system for identification of all yeast isolates (98.8% versus 95.4%,P<0.001 by Pearson's chi-square test) and for allCandidaandCandida-related species isolates (99.4% versus 95.5%,P< 0.001).

scholarly journals Comparison of the Vitek MS and Bruker Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Rhodococcus equi and Dietzia spp.

2017 ◽  
Vol 55 (7) ◽  
pp. 2255-2260 ◽  
Author(s):  
Carlos Ruiz de Alegría Puig ◽  
Lilian Pilares ◽  
Francesc Marco ◽  
Jordi Vila ◽  
Luis Martínez-Martínez ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Species Level ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Vitek Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

ABSTRACTRhodococcus equicauses pyogranulomatous pneumonia in domesticated animals and immunocompromised humans.Dietziaspp. are environmental bacteria that have rarely been associated with human infections.R. equiandDietziaspp. are closely related actinomycetes. Phenotypic discrimination betweenR. equiandDietziaon the basis of their Gram stain morphology and colony appearance is problematic. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) is a fast, reliable, and cost-effective method for identification of a wide variety of microorganisms. We have evaluated the performance of Bruker Biotyper versus that of Vitek MS for identification of a collection of 154 isolates identified at the source asR. equithat includes isolates belonging to the genusDietzia. PCR amplification of thechoEgene, encoding a cholesterol oxidase, and 16S rRNA sequencing were considered the reference methods forR. equiidentification. Biotyper identified 131 (85.1%) of the 154 isolates at the species level, and this figure increased to 152 (98.7%) when the species cutoff was reduced from a score of ≥2.000 to ≥1.750. Vitek MS correctly identified at the species level 130 (84.4%) isolates as long as bacteria were extracted with ethanol but only 35 (22.7%) isolates when samples were prepared by direct extraction from colonies. The two systems allowed differentiation betweenR. equiandDietziaspp., but identification of allDietziasp. isolates at the species level needed sequencing of the 16S rRNA gene.

scholarly journals Mycobacterium abscessus Complex Identification with Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

2015 ◽  
Vol 53 (7) ◽  
pp. 2355-2358 ◽  
Author(s):  
Theofano Panagea ◽  
David H. Pincus ◽  
Dorothy Grogono ◽  
Melissa Jones ◽  
Josephine Bryant ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Mycobacterium Abscessus ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

We determined that the Vitek MS Plus matrix-assisted laser desorption ionization–time of flight mass spectrometry using research-use-only (RUO) v.4.12 andin vitro-diagnostic (IVD) v.3.0 databases accurately identified 41Mycobacterium abscessussubsp.abscessusand 13M. abscessussubsp.massilienseisolates identified by whole-genome sequencing to the species but not the subspecies level, from Middlebrook 7H11 andBurkholderia cepaciaselective agars. Peak analysis revealed three peaks potentially able to differentiate between subspecies.

scholarly journals Misidentification of a Rare Species, Cryptococcus laurentii, by Commonly Used Commercial Biochemical Methods and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems: Challenges for Clinical Mycology Laboratories

2015 ◽  
Vol 54 (1) ◽  
pp. 226-229 ◽  
Author(s):  
Meng Xiao ◽  
Xin Fan ◽  
Xin-Xin Chen ◽  
He Wang ◽  
Li Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Cryptococcus Laurentii ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Forty-two putativeCryptococcus laurentiiisolates identified by the Vitek 2 system were collected in China. The gold standard, internal transcribed spacer (ITS) sequencing, confirmed that only two isolates were genuineC. laurentii. Bruker Biotyper matrix-assisted laser desorption ionization–time of flight mass spectrometry was able to identify theC. laurentiiisolates with an expanded custom database.

scholarly journals Identification of Neisseria gonorrhoeae by the Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System Is Improved by a Database Extension: TABLE 1

2016 ◽  
Vol 54 (4) ◽  
pp. 1130-1132 ◽  
Author(s):  
Valentijn A. Schweitzer ◽  
Alje P. van Dam ◽  
I Putu Yuda Hananta ◽  
Rob Schuurman ◽  
Johannes G. Kusters ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Neisseria Gonorrhoeae ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Identification ofNeisseria gonorrhoeaeby the Bruker matrix-assisted laser desorption ionization−time of flight mass spectrometry (MALDI-TOF MS) system may be affected by “B consistency categorization.” A supplementary database of 17N. gonorrhoeaemain spectra was constructed. Twelve of 64N. gonorrhoeaeidentifications were categorized with B consistency, which disappeared using the supplementary database. Database extension did not result in misidentification ofNeisseria meningitidis.

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