scholarly journals Pyrosequencing for Rapid Molecular Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Strains and Clinical Specimens

2011 ◽  
Vol 49 (10) ◽  
pp. 3683-3686 ◽  
Author(s):  
N. Garcia-Sierra ◽  
A. Lacoma ◽  
C. Prat ◽  
L. Haba ◽  
J. Maldonado ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Molecular Detection ◽  
Isoniazid Resistance ◽  
Clinical Specimens

scholarly journals Molecular detection of Mycobacterium tuberculosis in poor-quality cough specimens

2020 ◽  
Vol 69 (9) ◽  
pp. 1179-1182
Author(s):  
Tondani A. Mboneni ◽  
Owen O. Eales ◽  
Ntsoaki L. Mosina ◽  
P. Bernard Fourie
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Type Species ◽  
Molecular Detection ◽  
Molecular Transport ◽  
Poor Quality ◽  
Rt Pcr ◽  
Missed Opportunities ◽  
Content Type ◽  
Clinical Specimens ◽  
Enhanced Sensitivity

Clinical specimens unfit for laboratory processing represent missed opportunities for diagnosing tuberculosis. Poor-quality cough specimens (n=61) from presumptive tuberculosis cases were cultured and GeneXpert MTB/RIF (Xpert) successfully performed on samples transferred by flocked swab into PrimeStore molecular transport medium (PS-MTM). Mycobacterium tuberculosis was grown in culture from 13 (21.3 %) and Xpert reported 15 (24.2 %) positive, of which 10 concordant. RT-PCR of PS-MTM samples showed enhanced sensitivity; three positives were missed by Xpert, five by culture and three more detected for a total of 21 positives (34.4 %).

scholarly journals Detection of mutations associated with isoniazid resistance in multidrug-resistant Mycobacterium tuberculosis clinical isolates

2014 ◽  
Vol 69 (9) ◽  
pp. 2369-2375 ◽  
Author(s):  
Tomasz Jagielski ◽  
Zofia Bakuła ◽  
Katarzyna Roeske ◽  
Michał Kamiński ◽  
Agnieszka Napiórkowska ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Isoniazid Resistance ◽  
Detection Of Mutations
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