scholarly journals Comparison of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Systems for Identification of Mycobacteria Using Simplified Protein Extraction Protocols

2013 ◽  
Vol 52 (1) ◽  
pp. 130-138 ◽  
Author(s):  
C. A. Mather ◽  
S. F. Rivera ◽  
S. M. Butler-Wu
Keyword(s):  
Mass Spectrometry ◽  
Protein Extraction ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Vitek Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals A Comprehensive Evaluation of the Bruker Biotyper MS and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Yeasts, Part of the National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) Study, 2012 to 2013

2016 ◽  
Vol 54 (5) ◽  
pp. 1376-1380 ◽  
Author(s):  
He Wang ◽  
Yan-Yan Fan ◽  
Timothy Kudinha ◽  
Zhi-Peng Xu ◽  
Meng Xiao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Vitek Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Among the 2,683 yeast isolates representing 41 different species (25CandidaandCandida-related species and 16 non-Candidayeast species) collected in the National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) program (2012 to 2013), the Bruker Biotyper MS matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) system exhibited significantly higher accuracy rates than the Vitek MS system for identification of all yeast isolates (98.8% versus 95.4%,P<0.001 by Pearson's chi-square test) and for allCandidaandCandida-related species isolates (99.4% versus 95.5%,P< 0.001).

scholarly journals Comparison of Saramis 4.12 and IVD 3.0 Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Mycobacteria from Solid and Liquid Culture Media

2017 ◽  
Vol 55 (7) ◽  
pp. 2045-2054 ◽  
Author(s):  
Caroline Leyer ◽  
Guillaume Gregorowicz ◽  
Faiza Mougari ◽  
Laurent Raskine ◽  
Emmanuelle Cambau ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Vitek Ms ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

ABSTRACTDuring the last decade, many investigators have studied matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) for identification of mycobacteria. Diverse and contradictory results indicated that optimal level for routine testing has not been reached yet. This work aimed to assess Vitek MS through two distinct versions, Saramis v4.12 RUO and the IVD v3.0, under conditions close to routine laboratory practice. Overall, 111 mycobacterial isolates were subjected to protein extraction and same spectra were matched against both databases. The IVD v3.0 database proved to be superior to Saramis v4.12 and its identification rates remarkably increased, from 67% to 94% for isolates grown on Middlebrook 7H10 solid medium and from 62% to 91% for isolates grown on mycobacterial growth indicator tube (MGIT) liquid medium. With this new version, IVD v3.0, MALDI-TOF MS might be integrated into routine clinical diagnostics, although molecular techniques remain mandatory in some cases.

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