Immunodetection of Pneumocystis carinii in bronchoalveolar lavage specimens compared with methenamine silver stain.

1996 ◽  
Vol 34 (3) ◽  
pp. 728-730 ◽  
Author(s):  
I Lautenschlager ◽  
O Lyytikainen ◽  
L Jokipii ◽  
A Jokipii ◽  
A Maiche ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Pneumocystis Carinii ◽  
Silver Stain ◽  
Methenamine Silver ◽  
Methenamine Silver Stain

Detection of human cytomegalovirus infection in paraffin sections by Grocott’s methenamine silver stain for light and electron microscopy

1990 ◽  
Vol 48 (3) ◽  
pp. 808-809
Author(s):  
K. Chien ◽  
Y.K. Kim ◽  
I.P. Shintaku ◽  
R.L. Van de Velde ◽  
R.C. Heusser ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Fungal Infections ◽  
Pneumocystis Carinii ◽  
Light And Electron Microscopy ◽  
Paraffin Sections ◽  
Silver Stain ◽  
Infected Cells ◽  
Methenamine Silver ◽  
Methenamine Silver Stain ◽  
Light Green

Grocott's methenamine silver stain (GMS) has been used histologically for identifying Pneumocystis carinii and fungal infections in paraffin sections of surgical specimens. However, current studies indicate that aggregates of human cytomegalovirus (HCMV) in the cytoplasm of infected cells are also stained by GMS and can be detected by LM. Since precipitated silver particles are electron dense, the presence of silver labelled HCMV virions can be easily located and confirmed by TEM.GMS and light green stained paraffin sections positive for HCMV can be processed for TEM either by conventional procedures or as follows: Coverslips are removed by immersion in xylene. A few drops of 1% 0s04, freshly dissolved in xylene, are then pipetted onto each slide for 5 minutes. Prolonged osmication is to be avoided as it will cause reduction of the silver deposits. Slides are then rinsed in xylene and changed to 100% acetone. Substitution of acetone for xylene prior to resin infiltration will produce a better quality block for sectioning. The sections are embedded, polymerized and subsequently separated from slides as previously described. The light green staining is retained in the epoxy block, which facilitates tissue orientation and selection of areas for thin-sectioning.

Distinctive, rapid, and easy labeling of diffuse plaques in the Alzheimer brains by a new methenamine silver stain

1990 ◽  
Vol 79 (5) ◽  
pp. 569-572 ◽  
Author(s):  
H. Yamaguchi ◽  
C. Haga ◽  
S. Hirai ◽  
Y. Nakazato ◽  
K. Kosaka
Keyword(s):  
Silver Stain ◽  
Methenamine Silver ◽  
Methenamine Silver Stain ◽  
Diffuse Plaques

scholarly journals Pneumocystis jirovecii Pneumonia in a HIV-Infected Patient with a CD4 Count Greater Than 400 Cells/μL and Atovaquone Prophylaxis

2020 ◽  
Vol 2020 ◽  
pp. 1-4
Author(s):  
Abigayle Sullivan ◽  
Theresa Lanham ◽  
Ronald Krol ◽  
Shilla Zachariah
Keyword(s):  
Highly Active Antiretroviral Therapy ◽  
Lung Biopsy ◽  
Cd4 Count ◽  
Pneumocystis Jirovecii ◽  
Pneumocystis Jirovecii Pneumonia ◽  
Silver Stain ◽  
Highly Active ◽  
History Of ◽  
Methenamine Silver ◽  
Methenamine Silver Stain

We describe a rare case of Pneumocystis jirovecii pneumonia (PCP) in a heterosexual man with a pertinent medical history of well-controlled human immunodeficiency virus (HIV) on highly active antiretroviral therapy (HAART) and PCP prophylaxis with atovaquone. The patient presented with recurrent shortness of breath, worsening malaise, and fever, following treatment for hypersensitivity pneumonitis one month prior, including a twenty-four-day course of 40 milligrams daily glucocorticoid with taper. However, transbronchial biopsies, lavage, and cytology from prior admission were inconclusive. The patient refused video-assisted thoracic surgery (VATS) at that time. Upon readmission, bronchoscopy with right VATS and lung biopsy were performed. Grocott’s methenamine silver stain of right lung biopsy was positive for Pneumocystis jirovecii. This case is a rare example of PCP in a patient with a normal CD4 count (>487 cells/μL) and a low viral load (<20 copies/mL) despite PCP prophylactic antibiotics in the setting of recent iatrogenic immunosuppression.

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