scholarly journals Comparison of Two Enzyme-Linked Immunosorbent Assays and One Rapid Immunoblot Assay for Detection of Herpes Simplex Virus Type 2-Specific Antibodies in Serum

1998 ◽  
Vol 36 (3) ◽  
pp. 845-847 ◽  
Author(s):  
Jan Groen ◽  
Grietje Van Dijk ◽  
Hubert G. M. Niesters ◽  
Willem I. Van Der Meijden ◽  
Albert D. M. E. Osterhaus
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Serum Samples ◽  
Immunoblot Assay ◽  
Specific Antibodies ◽  
Immunosorbent Assays ◽  
Simplex Virus

The sensitivities and specificities of three immunoassays for the detection of herpes simplex virus type 2 (HSV-2)-specific immunoglobulin G antibodies in serum, including the one-strip rapid immunoblot assay (RIBA; Chiron Corporation) and two indirect enzyme immunosorbent assays (EIA; Gull Laboratories and Centocor), were compared by testing a panel of 1,250 serum samples from individuals attending an outpatient clinic for sexually transmitted diseases. A qualitative agreement among the three assays was observed with 1,080 serum samples (86.4%); 291 of the serum samples (23.3%) were positive, 789 samples (63.1%) were negative, and 170 serum samples (13.6%) gave a discordant result. Results were considered conclusive when a concordant result was obtained with two of three assays. The sensitivities and specificities of the RIBA, the Gull EIA, and the Centocor EIA proved to be 99.2, 99.7, and 89.9% and 97.1, 96.7, and 99.3%, respectively. These results indicate that the Chiron RIBA and the Gull EIA are especially useful and reliable for the detection of HSV-2-specific antibodies in serum.

scholarly journals Premarket Evaluation of a Commercial Glycoprotein G-Based Enzyme Immunoassay for Herpes Simplex Virus Type-Specific Antibodies

1998 ◽  
Vol 36 (1) ◽  
pp. 294-295 ◽  
Author(s):  
Rhoda L. Ashley ◽  
Linxian Wu ◽  
Jerry W. Pickering ◽  
Ming-Chieh Tu ◽  
Laura Schnorenberg
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Enzyme Immunoassay ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Glycoprotein G ◽  
Simplex Virus ◽  
Hsv 1

A new commercial glycoprotein G-based enzyme immunoassay (gG-EIA) was compared with Western blotting (WB) for detection of herpes simplex virus type 1 (HSV-1) or HSV-2 type-specific antibodies in 193 serum samples. Sensitivity for HSV-1 was 95%; specificity was 96%. Sensitivity for HSV-2 was 98%; specificity was 97%. Twelve of 13 serum samples with equivocal gG-EIA results were serotyped by WB.

scholarly journals Evaluation of Confirmatory Strategies for Detection of Type-Specific Antibodies against Herpes Simplex Virus Type 2

2002 ◽  
Vol 40 (2) ◽  
pp. 407-413 ◽  
Author(s):  
B. R. Eing ◽  
L. Lippelt ◽  
E. U. Lorentzen ◽  
W. Hafezi ◽  
W. Schlumberger ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Specific Antibodies ◽  
Simplex Virus

scholarly journals Performance of the BioPlex 2200 Multiplexing Immunoassay Platform for the Detection of Herpes Simplex Virus Type 2 Specific Antibodies in African Settings

2011 ◽  
Vol 18 (7) ◽  
pp. 1191-1193 ◽  
Author(s):  
Jérôme LeGoff ◽  
Gérard Grésenguet ◽  
Chrysostome Gody ◽  
Jean De Dieu Longo ◽  
Nzambi Khonde ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Sub Saharan Africa ◽  
Specific Antibodies ◽  
Sub Saharan ◽  
Simplex Virus ◽  
Herpes Simplex Virus 2

ABSTRACTThe BioPlex platform was evaluated for the detection of herpes simplex virus 2 (HSV-2) antibodies in sub-Saharan Africa individuals in comparison to clinicovirological standards and compared to HerpeSelect. The sensitivities and specificities were, respectively, 88.9% and 93.5% for BioPlex and 89.9% and 92.7% for HerpeSelect. The agreement between both assays was 95.7%.

scholarly journals Performance of Two Commercial Enzyme-Linked Immunosorbent Assay Kits Using Recombinant Glycoprotein G2 Antigen for Detection of Herpes Simplex Virus Type 2 Specific Antibodies

2005 ◽  
Vol 12 (2) ◽  
pp. 359-360 ◽  
Author(s):  
Sharmila M. Reddy ◽  
P. Balakrishnan ◽  
S. Uma ◽  
S. P. Thyagarajan ◽  
Suniti Solomon
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Immunosorbent Assay ◽  
Herpes Simplex Virus Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Western Blot Assay ◽  
Simplex Virus

ABSTRACT For 93 stored serum samples tested by HerpeSelect2 and the Euroimmun enzyme-linked immunosorbent assay for detection of herpes simplex virus type 2-specific immunoglobulin G antibodies, the concordance of positive and negative results was 100%. Moreover, all the results that were equivocal by HerpeSelect2 (negative by Euroimmun) were confirmed as being negative by a Western blot assay.

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