scholarly journals Rapid fluorescent-antibody method with bromelase for identification of group A streptococci

1977 ◽  
Vol 5 (2) ◽  
pp. 255-256
Author(s):  
C A Waters ◽  
M A Makens
Keyword(s):  
Staining Method ◽  
Fluorescent Antibody ◽  
Group A Streptococci ◽  
Simple Method ◽  
Antibody Staining ◽  
Antibody Method ◽  
Nonspecific Staining ◽  
Group A ◽  
Method Group ◽  
Fluorescent Antibody Method

This is a preliminary report on a rapid fluorescent-antibody staining method utilizing the enzyme Bromelase that effectively eliminates nonspecific staining of staphylococci. By this simple method, group A streptococci were accurately identified from 2- to 4-h broth cultures.

Identification of Group A Beta-Hemolytic Streptococci by the Fluorescent Antibody Method

1978 ◽  
Vol 143 (11) ◽  
pp. 782-784
Author(s):  
Jerome B. Myers ◽  
John W. Crum ◽  
Lynn P. Beaulieu
Keyword(s):  
Fluorescent Antibody ◽  
Antibody Method ◽  
Group A ◽  
Fluorescent Antibody Method

scholarly journals Rapid grouping of beta-hemolytic streptococci by latex agglutination

1978 ◽  
Vol 8 (3) ◽  
pp. 326-328
Author(s):  
Y A Lue ◽  
I P Howit ◽  
P D Ellner
Keyword(s):  
Fluorescent Antibody ◽  
Latex Agglutination ◽  
Group A Streptococci ◽  
Cross Reactions ◽  
Antibody Staining ◽  
Group A ◽  
Group B ◽  
Fluorescent Antibody Staining

Latex agglutination was compared with fluorescent-antibody staining with group A conjugate and Lancefield precipitation for grouping of beta-hemolytic streptococci. Latex agglutination correctly grouped 98.8% of 82 group A streptococci and more than 95% of 187 group B, C, or G streptococci. Occasional cross-reactions occurred between groups A and C and groups B and G.

Localization of pancreatic enzymes in ultrathin frozen sections stained with metal labeled antibody

1978 ◽  
Vol 36 (2) ◽  
pp. 90-91
Author(s):  
Kenjiro Yasuda
Keyword(s):  
Fluorescent Antibody ◽  
Pancreatic Enzymes ◽  
Tissue Preparation ◽  
Zymogen Granules ◽  
Frozen Sections ◽  
En Bloc ◽  
Antibody Method ◽  
Ultrathin Frozen Sections ◽  
Fluorescent Antibody Method

Localization of amylase,chymotrypsinogen and trypsinogen in pancreas was demonstrated by Yasuda and Coons (1966), by using fluorescent antibody method. These enzymes were naturally found in the zymogen granules. Among them, amylase showed a diffuse localization around the nucleus, in addition to the zymogen granules. Using ferritin antibody method, scattered ferritin granules were also found around the Golgi area (Yasuda et al.,1967). The recent advance in the tissue preparation enables the antigen to be localized in the ultrathin frozen sections, by applying the labeled antibodies onto the sections instead of staining the tissue en bloc.The present study deals with the comparison of the localization of amylase and lipase demonstrated by applying the bismuth-labeled, peroxidase-labeled and ferritin-labeled antibody methods on the ultrathin frozen sections of pancreas, and on the blocks of the same tissue.

Sign in / Sign up

Export Citation Format

Share Document