scholarly journals Activity of simian DNA-binding factors is altered in the presence of simian virus 40 (SV40) early proteins: characterization of factors binding to elements involved in activation of the SV40 late promoter.

1990 ◽  
Vol 64 (1) ◽  
pp. 173-184 ◽  
Author(s):  
G J Gallo ◽  
M C Gruda ◽  
J R Manuppello ◽  
J C Alwine
Keyword(s):  
Dna Binding ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Late Promoter ◽  
Early Proteins ◽  
Dna Binding Factors

Simian virus 40 T antigen alters the binding characteristics of specific simian DNA-binding factors

1988 ◽  
Vol 8 (4) ◽  
pp. 1648-1656
Author(s):  
G J Gallo ◽  
G Gilinger ◽  
J C Alwine
Keyword(s):  
Dna Binding ◽  
Binding Sites ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Late Promoter ◽  
Promoter Elements ◽  
Binding Characteristics ◽  
Dna Binding Factors ◽  
Cellular Dna

The late promoter of simian virus 40 is transcriptionally activated, in trans, by large T antigen, the primary viral early gene product. Although large T antigen is a well-characterized DNA-binding protein, a variety of data suggest that its trans-activation function does not require direct interaction with DNA. We demonstrate that defined late promoter elements, omega (omega), tau (tau), and delta (delta), necessary for T-antigen-mediated trans-activation, are binding sites for simian cellular factors, not T antigen. Two of the late promoter elements (omega and tau) are shown to bind the same factor or family of factors. These factors bind to a site very similar to that for the HeLa cell factor AP1. We refer to these factors as the simian AP1-sequence recognition proteins (sAP1-SRPs). Compared with normal simian CV-1P cells, the sAP1-SRPs from T-antigen-producing COS cells, or from 14-h simian virus 40-infected CV-1P cells, showed altered binding patterns to both the omega and tau binding sites. In addition, the sAP1-SRPs from T-antigen-containing cells bound to the tau site more stably than did the analogous factors from normal CV-1P cells. The altered pattern of binding and the increased stability of binding correlated with the presence of T antigen in the cell. Additionally, the alteration of the binding pattern within 14 h of infection in CV-1P cells is temporally correct for late promoter activation. Overall, the data show (i) that the late promoter elements necessary for T-antigen-mediated trans-activation contain binding sites for simian cellular DNA-binding proteins; (ii) that the presence of T antigen causes alterations in the binding characteristics of specific simian cellular DNA-binding factors or families of factors; and (iii) that factors which bind to the late promoter elements required for activation have altered and more stable binding characteristics in the presence of T antigen. These points strongly suggest that T antigen mediates trans-activation indirectly through the alteration of binding of at least one specific simian cellular factor, sAP1-SRP, or through the induction of a family of sAP1-SRP factors.

scholarly journals Simian virus 40 T antigen alters the binding characteristics of specific simian DNA-binding factors.

1988 ◽  
Vol 8 (4) ◽  
pp. 1648-1656 ◽  
Author(s):  
G J Gallo ◽  
G Gilinger ◽  
J C Alwine
Keyword(s):  
Dna Binding ◽  
Binding Sites ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Late Promoter ◽  
Promoter Elements ◽  
Binding Characteristics ◽  
Dna Binding Factors ◽  
Cellular Dna

The late promoter of simian virus 40 is transcriptionally activated, in trans, by large T antigen, the primary viral early gene product. Although large T antigen is a well-characterized DNA-binding protein, a variety of data suggest that its trans-activation function does not require direct interaction with DNA. We demonstrate that defined late promoter elements, omega (omega), tau (tau), and delta (delta), necessary for T-antigen-mediated trans-activation, are binding sites for simian cellular factors, not T antigen. Two of the late promoter elements (omega and tau) are shown to bind the same factor or family of factors. These factors bind to a site very similar to that for the HeLa cell factor AP1. We refer to these factors as the simian AP1-sequence recognition proteins (sAP1-SRPs). Compared with normal simian CV-1P cells, the sAP1-SRPs from T-antigen-producing COS cells, or from 14-h simian virus 40-infected CV-1P cells, showed altered binding patterns to both the omega and tau binding sites. In addition, the sAP1-SRPs from T-antigen-containing cells bound to the tau site more stably than did the analogous factors from normal CV-1P cells. The altered pattern of binding and the increased stability of binding correlated with the presence of T antigen in the cell. Additionally, the alteration of the binding pattern within 14 h of infection in CV-1P cells is temporally correct for late promoter activation. Overall, the data show (i) that the late promoter elements necessary for T-antigen-mediated trans-activation contain binding sites for simian cellular DNA-binding proteins; (ii) that the presence of T antigen causes alterations in the binding characteristics of specific simian cellular DNA-binding factors or families of factors; and (iii) that factors which bind to the late promoter elements required for activation have altered and more stable binding characteristics in the presence of T antigen. These points strongly suggest that T antigen mediates trans-activation indirectly through the alteration of binding of at least one specific simian cellular factor, sAP1-SRP, or through the induction of a family of sAP1-SRP factors.

Characterization of a new tissue-specific transcription factor binding to the simian virus 40 enhancer TC-II (NF-kappa B) element

1992 ◽  
Vol 12 (11) ◽  
pp. 5217-5227
Author(s):  
A L Lattion ◽  
E Espel ◽  
P Reichenbach ◽  
C Fromental ◽  
P Bucher ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
B Lymphocytes ◽  
Binding Proteins ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Dna Binding Proteins ◽  
Nf Kappa B ◽  
Dna Binding Specificity

We have biochemically and functionally characterized a new transcription factor, NP-TCII, which is present in nuclei from unstimulated T and B lymphocytes but is not found in nonhematopoietic cells. This factor has a DNA-binding specificity similar to that of NF-kappa B but is unrelated to this or other Rel proteins by functional and biochemical criteria. It can also be distinguished from other previously described lymphocyte-specific DNA-binding proteins.

scholarly journals Characterization of a new tissue-specific transcription factor binding to the simian virus 40 enhancer TC-II (NF-kappa B) element.

1992 ◽  
Vol 12 (11) ◽  
pp. 5217-5227 ◽  
Author(s):  
A L Lattion ◽  
E Espel ◽  
P Reichenbach ◽  
C Fromental ◽  
P Bucher ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dna Binding ◽  
B Lymphocytes ◽  
Binding Proteins ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Dna Binding Proteins ◽  
Nf Kappa B ◽  
Dna Binding Specificity

We have biochemically and functionally characterized a new transcription factor, NP-TCII, which is present in nuclei from unstimulated T and B lymphocytes but is not found in nonhematopoietic cells. This factor has a DNA-binding specificity similar to that of NF-kappa B but is unrelated to this or other Rel proteins by functional and biochemical criteria. It can also be distinguished from other previously described lymphocyte-specific DNA-binding proteins.

scholarly journals Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.

1997 ◽  
Vol 71 (5) ◽  
pp. 3972-3985 ◽  
Author(s):  
W S Joo ◽  
X Luo ◽  
D Denis ◽  
H Y Kim ◽  
G J Rainey ◽  
...  
Keyword(s):  
Dna Binding ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Sv40 T Antigen ◽  
Sv40 Origin

scholarly journals Characterization of a minimal simian virus 40 late promoter: enhancer elements in the 72-base-pair repeat not required.

1989 ◽  
Vol 63 (3) ◽  
pp. 1420-1427 ◽  
Author(s):  
W S Dynan ◽  
S A Chervitz
Keyword(s):  
Base Pair ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Late Promoter

Interaction between T antigen and TEA domain of the factor TEF-1 derepresses simian virus 40 late promoter in vitro: identification of T-antigen domains important for transcription control.

1996 ◽  
Vol 70 (2) ◽  
pp. 1203-1212 ◽  
Author(s):  
L C Berger ◽  
D B Smith ◽  
I Davidson ◽  
J J Hwang ◽  
E Fanning ◽  
...  
Keyword(s):  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Late Promoter ◽  
Transcription Control

scholarly journals Identification of a DNA binding domain in simian virus 40 capsid proteins Vp2 and Vp3.

1993 ◽  
Vol 268 (28) ◽  
pp. 20877-20883
Author(s):  
J Clever ◽  
D.A. Dean ◽  
H Kasamatsu
Keyword(s):  
Dna Binding ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Binding Domain ◽  
Capsid Proteins ◽  
Dna Binding Domain
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