The role of the pseudoknot at the 3' end of turnip yellow mosaic virus RNA in minus-strand synthesis by the viral RNA-dependent RNA polymerase.

1997 ◽  
Vol 71 (8) ◽  
pp. 5990-5996 ◽  
Author(s):  
B A Deiman ◽  
R M Kortlever ◽  
C W Pleij
Keyword(s):  
Rna Polymerase ◽  
Mosaic Virus ◽  
Viral Rna ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Minus Strand ◽  
Rna Dependent Rna Polymerase ◽  
Virus Rna

scholarly journals The Ubiquitin-Proteasome System Regulates the Accumulation of Turnip yellow mosaic virus RNA-Dependent RNA Polymerase during Viral Infection

2010 ◽  
Vol 22 (9) ◽  
pp. 3142-3152 ◽  
Author(s):  
Laurent Camborde ◽  
Séverine Planchais ◽  
Vincent Tournier ◽  
Anna Jakubiec ◽  
Gabrièle Drugeon ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Viral Infection ◽  
Mosaic Virus ◽  
Ubiquitin Proteasome System ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Rna Dependent Rna Polymerase ◽  
Virus Rna ◽  
Ubiquitin Proteasome

scholarly journals Minimal Template Requirements for Initiation of Minus-Strand Synthesis In Vitro by the RNA-Dependent RNA Polymerase of Turnip Yellow Mosaic Virus

1998 ◽  
Vol 72 (5) ◽  
pp. 3965-3972 ◽  
Author(s):  
B. A. L. M. Deiman ◽  
A. K. Koenen ◽  
P. W. G. Verlaan ◽  
C. W. A. Pleij
Keyword(s):  
Rna Polymerase ◽  
Mosaic Virus ◽  
Mutational Analysis ◽  
Yellow Mosaic Virus ◽  
Turnip Yellow Mosaic Virus ◽  
Minus Strand ◽  
Rna Dependent Rna Polymerase ◽  
Substitution Mutations ◽  
Internal Initiation

ABSTRACT From mutational analysis of the 3′-terminal hairpin of turnip yellow mosaic virus (TYMV) RNA and use of nonstructured C-rich RNA templates, we conclude that the main determinant in the tRNA-like structure of TYMV RNA for initiation of minus-strand synthesis by the viral RNA-dependent RNA polymerase (RdRp) is the non-base-paired 3′ ACC(A) end. Base pairing of this 3′ end reduces the transcription efficiency drastically, and deletion of only the 3′-terminal A residue results in a fivefold drop in efficiency. The two C residues of the 3′ ACCA end are required for efficient transcription, as shown by substitution mutations. However, the 5′ A residue is not specifically involved in initiation of transcription, as shown by substitution mutations. Furthermore, the hairpin stem and loop upstream of the 3′ ACCA end also do not interact with the RdRp in a base-specific way. However, for efficient transcription, the hairpin stem should be at least five bp in length, while the calculated ΔG value should be less than −10.5 kcal/mol. Unexpectedly, the use of nonstructured C-rich RNA templates showed that the RdRp can start internally on an NCCN or NUCN sequence. Therefore, a possible function of the tRNA-like structure of TYMV RNA may be to prevent internal initiation of minus-strand synthesis.

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