scholarly journals Sequences 5′ of the Conserved tRNA-like Promoter Modulate the Initiation of Minus-Strand Synthesis by the Brome Mosaic Virus RNA-Dependent RNA Polymerase

Virology ◽  
1998 ◽  
Vol 252 (2) ◽  
pp. 458-467 ◽  
Author(s):  
Matthew R. Chapman ◽  
A.L.N. Rao ◽  
C.Cheng Kao
Keyword(s):  
Rna Polymerase ◽  
Mosaic Virus ◽  
Brome Mosaic Virus ◽  
Minus Strand ◽  
Rna Dependent Rna Polymerase ◽  
Virus Rna

scholarly journals Initiation of Genomic Plus-Strand RNA Synthesis from DNA and RNA Templates by a Viral RNA-Dependent RNA Polymerase

1999 ◽  
Vol 73 (8) ◽  
pp. 6415-6423 ◽  
Author(s):  
K. Sivakumaran ◽  
C. Cheng Kao
Keyword(s):  
Rna Polymerase ◽  
Mosaic Virus ◽  
Rna Synthesis ◽  
Mutational Analysis ◽  
Initiation Site ◽  
Brome Mosaic Virus ◽  
Minus Strand ◽  
Rna Dependent Rna Polymerase ◽  
Dna And Rna

ABSTRACT In contrast to the synthesis of minus-strand genomic and plus-strand subgenomic RNAs, the requirements for brome mosaic virus (BMV) genomic plus-strand RNA synthesis in vitro have not been previously reported. Therefore, little is known about the biochemical requirements for directing genomic plus-strand synthesis. Using DNA templates to characterize the requirements for RNA-dependent RNA polymerase template recognition, we found that initiation from the 3′ end of a template requires one nucleotide 3′ of the initiation nucleotide. The addition of a nontemplated nucleotide at the 3′ end of minus-strand BMV RNAs led to initiation of genomic plus-strand RNA in vitro. Genomic plus-strand initiation was specific since cucumber mosaic virus minus-strand RNA templates were unable to direct efficient synthesis under the same conditions. In addition, mutational analysis of the minus-strand template revealed that the −1 nontemplated nucleotide, along with the +1 cytidylate and +2 adenylate, is important for RNA-dependent RNA polymerase interaction. Furthermore, genomic plus-strand RNA synthesis is affected by sequences 5′ of the initiation site.

scholarly journals Characterization of a host protein associated with brome mosaic virus RNA-dependent RNA polymerase.

1993 ◽  
Vol 90 (4) ◽  
pp. 1498-1502 ◽  
Author(s):  
R. Quadt ◽  
C. C. Kao ◽  
K. S. Browning ◽  
R. P. Hershberger ◽  
P. Ahlquist
Keyword(s):  
Rna Polymerase ◽  
Mosaic Virus ◽  
Brome Mosaic Virus ◽  
Host Protein ◽  
Rna Dependent Rna Polymerase ◽  
Virus Rna
Sign in / Sign up

Export Citation Format

Share Document