scholarly journals Characterization of a Novel Close-to-Root Papillomavirus from a Florida Manatee by Using Multiply Primed Rolling-Circle Amplification: Trichechus manatus latirostris Papillomavirus Type 1

2004 ◽  
Vol 78 (22) ◽  
pp. 12698-12702 ◽  
Author(s):  
Annabel Rector ◽  
Gregory D. Bossart ◽  
Shin-Je Ghim ◽  
John P. Sundberg ◽  
A. Bennett Jenson ◽  
...  
Keyword(s):  
Marine Mammals ◽  
Rolling Circle Amplification ◽  
Phylogenetic Position ◽  
Florida Manatee ◽  
Trichechus Manatus ◽  
Trichechus Manatus Latirostris ◽  
Rolling Circle ◽  
Amplification Protocol

ABSTRACT By using an isothermal multiply primed rolling-circle amplification protocol, the complete genomic DNA of a novel papillomavirus was amplified from a skin lesion biopsy of a Florida manatee (Trichechus manatus latirostris), one of the most endangered marine mammals in United States coastal waters. The nucleotide sequence, genome organization, and phylogenetic position of the Trichechus manatus latirostris papillomavirus type 1 (TmPV-1) were determined. TmPV-1 is the first virus isolated from the order of Sirenia. A phylogenetic analysis shows that TmPV-1 is only distantly related to other papillomavirus sequences, and it appears in our phylogenetic tree as a novel close-to-root papillomavirus genus.

scholarly journals Molecular characterization of novel mucosotropic papillomaviruses from a Florida manatee (Trichechus manatus latirostris)

2015 ◽  
Vol 96 (12) ◽  
pp. 3545-3553 ◽  
Author(s):  
Maryam Zahin ◽  
Shin-je Ghim ◽  
Sujita Khanal ◽  
Gregory D. Bossart ◽  
Alfred B. Jenson ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Genomic Organization ◽  
Pairwise Alignment ◽  
Full Genome ◽  
Coding Region ◽  
Florida Manatee ◽  
Trichechus Manatus ◽  
Trichechus Manatus Latirostris ◽  
Sequence Similarities

We isolated two new manatee papillomavirus (PV) types, TmPV3 and TmPV4, from a Florida manatee (Trichechus manatus latirostris). Two PV types were previously isolated from this species. TmPV1 is widely dispersed amongst manatees and a close-to-root PV; not much is known about TmPV2. The genomes of TmPV3 and TmPV4 were 7622 and 7771 bp in size, respectively. Both PVs had a genomic organization characteristic of all PVs, with one non-coding region and seven ORFs, including the E7 ORF that is absent in other cetacean PVs. Although these PVs were isolated from separate genital lesions of the same manatee, an enlarged E2/E4 ORF was found only in the TmPV4 genome. The full genome and L1 sequence similarities between TmPV3 and TmPV4 were 63.2 and 70.3 %, respectively. These genomes shared only 49.1 and 50.2 % similarity with TmPV1. The pairwise alignment of L1 nucleotide sequences indicated that the two new PVs nested in a monophyletic group of the genus Rhopapillomavirus, together with the cutaneotropic TmPV1 and TmPV2.

ISOLATION AND CHARACTERIZATION OF A cDNA ENCODING INTERLEUKIN 2 FROM THE FLORIDA MANATEE, TRICHECHUS MANATUS LATIROSTRIS

1996 ◽  
Vol 12 (1) ◽  
pp. 89-98 ◽  
Author(s):  
Mary E. Cashman ◽  
Traci L. Ness ◽  
William B. Roess ◽  
W. Guy Bradley ◽  
John E. Reynolds
Keyword(s):  
Interleukin 2 ◽  
Florida Manatee ◽  
Trichechus Manatus ◽  
Trichechus Manatus Latirostris ◽  
Isolation And Characterization

Touch, Hearing, and Vision of the Florida Manatee, Trichechus manatus latirostris

2010 ◽  
Author(s):  
Gordon B. Bauer ◽  
Joseph C. Gaspard ◽  
Debborah E. Colbert ◽  
Roger L. Reep ◽  
David Mann
Keyword(s):  
Florida Manatee ◽  
Trichechus Manatus ◽  
Trichechus Manatus Latirostris

scholarly journals Isolation and cloning of a papillomavirus from a North American porcupine by using multiply primed rolling-circle amplification: the Erethizon dorsatum papillomavirus type 1

Virology ◽  
2005 ◽  
Vol 331 (2) ◽  
pp. 449-456 ◽  
Author(s):  
Annabel Rector ◽  
Ruth Tachezy ◽  
Koenraad Van Doorslaer ◽  
Tracey MacNamara ◽  
Robert D. Burk ◽  
...  
Keyword(s):  
North American ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Erethizon Dorsatum
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