First Report of Ixodes scapularis Ticks Parasitizing a North American Porcupine in Canada

Adult females of the blacklegged tick, Ixodes scapularis (Acari: Ixodidae), were collected from a North American porcupine, Erethizon dorsatum, in eastern Ontario, Canada. This porcupine parasitism indicates that an established population of I. scapularis is present in the local vicinity. This tick species is known to parasitize more than 150 different vertebrate hosts, including the North American porcupine. The presence of I. scapularis ticks parasitizing a North American porcupine constitutes a new tick-host record in Canada.

HOME RANGE AND HABITAT SELECTION OF CAVE-DWELLING NORTH AMERICAN PORCUPINES (ERETHIZON DORSATUM) IN CENTRAL TEXAS

North American porcupines (Erethizon dorsatum) have expanded their range into central Texas and are now frequent users of caves as den sites. What remains unknown is how caves affect their home range, and their local habitat preferences. This information is important for management decisions on Joint Base San Antonio – Camp Bullis where novel and abundant porcupine scat in caves could jeopardize federally endangered cave-obligate arthropods by allowing for the invasion of less specialized terrestrial species. To better understand porcupine home range and habitat use at Camp Bullis, we trapped four porcupines at cave entrances and fitted them with GPS collars. The 95% home range averaged 71.3 ha for females and measured 420.6 ha for the male. The 50% core habitat averaged 55.4 ha for females and measured 7.4 ha for the male. Porcupines typically stayed near the den-cave trap site except when visiting more diverse mixed forest patches. At the landscape and point levels, individuals selected for forested cover and avoided open areas. At the home range level, individuals selected for bare ground and roads, which were likely used to get from the cave den site to feed at mixed forest patches. Typically solitary, individuals in this study tolerated sharing a cave. Because of the small sample size and single sampling location, this study represents a pilot study and additional research is needed to establish concrete conclusions. Should cave managers need to limit the cave use by porcupines, a cave gate, exclosure, or reduction of forested cover would make caves less desirable.

Complete genome analysis confirms that the pygmy marmoset adenovirus is a variant of the skunk adenovirus 1 – Short communication

The complete genomic sequence along with phylogenetic analyses of an adenovirus (AdV), isolated from a dead captive pygmy marmoset (Callithrix pygmaea) from a Hungarian zoo is reported. Earlier, based on the phylogenetic analysis of the sequence of a PCR-amplified fragment from the DNA polymerase gene, the pygmy marmoset AdV (PMAdV) has been reported to cluster closest to certain chiropteran AdVs. In the following years similar AdVs were discovered in additional mammalian hosts, including a skunk (Mephitis mephitis), African pygmy hedgehogs (Atelerix albiventris), North American porcupines (Erethizon dorsatum) and grey fox (Urocyon cinereoargenteus). After the full genome analysis of the skunk adenovirus (SkAdV-1), a novel species Skunk mastadenovirus A (SkAdV-A) has been established. The AdVs, originating from the African pygmy hedgehogs, have been found to belong to virus species SkAdV-A. Partial gene sequences from the porcupine AdVs have also implied their very close genetic relatedness to SkAdV-A. The complete genomic sequence of PMAdV, examined in this study, was found to share 99.83% nucleotide identity with SkAdV-1, thus unequivocally represents a genomic variant of SkAdV-1. The observation that viruses classifiable as SkAdV-A are able to infect and cause diseases in several, distantly related mammals seems to deserve further studies to elucidate the infection biology of this intriguing AdV.

Atypical Dermatophytosis in 12 North American Porcupines (Erethizon dorsatum) from the Northeastern United States 2010–2017

Twelve wild North American porcupines (Erethizon dorsatum) out of a total of 44 of this species examined in an 8-year period were diagnosed with dermatopathies while being cared for at two wildlife rehabilitation clinics. Biopsy and necropsy were performed on seven and five animals, respectively. Atypical dermatophytosis was diagnosed in all cases. Lesions consisted of diffuse severe epidermal hyperkeratosis and mild hyperplasia with mild lymphoplasmacytic dermatitis and no folliculitis. Dermatophytes were noted histologically as hyphae and spores in hair shafts, and follicular and epidermal keratin. Trichophyton sp. was grown in 5/6 animals where culture was performed, with a molecular diagnosis of Arthroderma benhamiae/Trichophyton mentagrophytes in these five cases. Metagenomic analysis of formalin-fixed paraffin-embedded tissue samples from three cases identified fungi from 17 orders in phyla Basidiomycota and Ascomycota. Alteration of therapy from ketaconazole, which was unsuccessful in four out of five early cases, to terbinafine or nitraconazole led to the resolution of disease and recovery to release in four subsequent animals. In all, six animals were euthanized or died due to dermatopathy, no cases resolved spontaneously, and six cases were resolved with therapy. The work we present demonstrates an atypical lesion and anatomical distribution due to dermatophytosis in a series of free-ranging wild porcupines and the successful development of novel techniques for extracting and sequencing nucleic acids from fungus in archival formalin-fixed paraffin-embedded animal tissue.

Behaviour of a porcupine (Erethizon dorsatum) swimming across a small boreal stream

The swimming behaviour of North American Porcupine (Erethizon dorsatum) is largely unrecorded, even though much of its habitat is bisected by innumerable rivers and streams. Moreover, the literature is inconsistent regarding how readily porcupines take to the water and how well adapted they are for swimming. I observed a porcupine swimming across a relatively placid and shallow braid in the Klondike River (Yukon, Canada), after it had aborted three apparent attempts to swim at a relatively fast-flowing, deep channel upstream. This observation provides evidence of porcupine swimming across moving water and suggests that they may be reluctant to do so and selective of where they cross rivers and streams.

Atypical Dermatophytosis in 12 North American Porcupines (Erethizon dorsatum) from the Northeastern United States 2010-2017

Twelve wild North American Porcupines (Erethizon dorsatum) were diagnosed with dermatopathies while being cared for at two wildlife rehabilitation clinics. Biopsy and necropsy were performed on 7 and 5 animals respectively. Atypical dermatophytosis was diagnosed in all cases. Lesions consisted of diffuse severe epidermal hyperkeratosis and mild hyperplasia, with mild lymphoplasmacytic dermatitis, and no folliculitis. Dermatophytes were noted histologically as hyphae and spores in hair shafts, and follicular and epidermal keratin. Trichophyton sp. was grown in 5/6 animals where culture was performed, with molecular diagnosis of Arthroderma benhamiae / Trichophyton mentagrophytes in these 5 cases. Metagenomic analysis of formalin-fixed paraffin-embedded tissue samples from 3 cases identified fungi from 17 orders in phyla Basidiomycota and Ascomycota. Alteration of therapy from ketaconazole, which was unsuccessful in 4 of 5 early cases, to terbinafine or nitraconazole lead to resolution of disease and recovery to release in four subsequent animals. In all, 6 animals were euthanized or died due to dermatopathy, no cases resolved spontaneously, and 6 cases resolved with therapy. The work we present demonstrates an atypical lesion and anatomical distribution due to dermatophytosis in a series of free-ranging wild porcupines and successful development of novel techniques for extraction and sequencing nucleic acids from fungus in archival formalin-fixed paraffin-embedded animal tissue.