scholarly journals The DNA-A Component of a Plant Geminivirus (Indian Mung Bean Yellow Mosaic Virus) Replicates in Budding Yeast Cells

2004 ◽  
Vol 78 (5) ◽  
pp. 2405-2413 ◽  
Author(s):  
Vineetha Raghavan ◽  
Punjab S. Malik ◽  
Nirupam Roy Choudhury ◽  
Sunil K. Mukherjee
Keyword(s):  
Dna Replication ◽  
Mosaic Virus ◽  
Plasmid Dna ◽  
Mung Bean ◽  
Model System ◽  
Bean Yellow Mosaic Virus ◽  
Yeast Cells ◽  
Yellow Mosaic Virus ◽  
Dna Viruses ◽  
Viral Factor

ABSTRACT Understanding the biochemistry of DNA replication of the plant DNA viruses is important for the development of antiviral strategies. Since DNA replication is little studied in plants, a genetically tractable, easily culturable, eukaryotic model system is required to pursue such studies in a facile manner. Here we report the development of a yeast model system that supports DNA replication of a chosen geminivirus strain, Indian mung bean yellow mosaic virus. The replication of plasmid DNA in the model system relies specifically on the virus-derived elements and factors. Usage of this model system revealed the role of at least one hitherto unknown viral factor for viral DNA replication. The episomal characteristic of single-strandedness of replicated plasmid DNA was shown, and the expression of viral genes was also confirmed. This model system is expected to shed light on the machinery and mechanism involved in geminiviral DNA replication in plants.

scholarly journals PCNA Interacts with Indian Mung Bean Yellow Mosaic Virus Rep and Downregulates Rep Activity

2004 ◽  
Vol 78 (21) ◽  
pp. 11890-11903 ◽  
Author(s):  
Basavaraj Bagewadi ◽  
Shoajiang Chen ◽  
Sunil K. Lal ◽  
Nirupam Roy Choudhury ◽  
Sunil K. Mukherjee
Keyword(s):  
Mosaic Virus ◽  
Mung Bean ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Nuclear Antigen ◽  
Resting Cells ◽  
Rolling Circle Replication ◽  
Proliferative Cell Nuclear Antigen ◽  
Bipartite Genome

ABSTRACT Proliferative cell nuclear antigen (PCNA), a conserved plant protein as well as an important replication factor, is induced in response to geminivirus infection in the resting cells of the phloem tissues. The biochemical role of PCNA in rolling circle replication (RCR) of geminivirus DNA has not been explored in detail. The initiation of RCR of the bipartite genome of a geminivirus, Indian mung bean yellow mosaic virus (IMYMV), is mainly controlled by viral protein Rep (or AL1 or AC1). The role of host PCNA in RCR of IMYMV was revealed by studying the physical and functional interactions between recombinant PCNA and recombinant IMYMV Rep. Pea nuclear PCNA as well as recombinant pea PCNA showed binding to recombinant Rep in experiments involving both affinity chromatography and yeast two-hybrid approaches. The contacting amino acid residues of PCNA seemed to be present throughout a wide region of the trimeric protein, while those of Rep appeared to be localized only in the middle part of the protein. The site-specific nicking-closing activity and the ATPase function of IMYMV Rep were impaired by PCNA. These observations lead to interesting speculations about the control of viral RCR and dynamic profiles of protein-protein interactions at the RCR origin of the geminiviruses.

Physical Mapping and Molecular Cloning of Mung Bean Yellow Mosaic Virus DNA

Intervirology ◽  
1990 ◽  
Vol 31 (1) ◽  
pp. 50-56 ◽  
Author(s):  
T. Morinaga ◽  
M. Ikegami ◽  
K. Miura
Keyword(s):  
Mosaic Virus ◽  
Molecular Cloning ◽  
Physical Mapping ◽  
Mung Bean ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus

Identification and Molecular Characterization of Mung Bean Yellow Mosaic Virus in French Bean through Coat Protein Gene

2020 ◽  
Author(s):  
T.C. Archith ◽  
V. Devappa ◽  
B. Manjunath ◽  
Chirag Reddy
Keyword(s):  
Coat Protein ◽  
Early Detection ◽  
Mosaic Virus ◽  
Molecular Characterization ◽  
Mung Bean ◽  
French Bean ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Cp Gene

Background: French bean (Phaseolus vulgaris L.), is one of the important legume vegetables grown in India. The disease mung bean yellow mosaic virus (MYMV) limits successful production of beans. MYMV has become an epidemic in bean growing regions, particularly in the tropical and subtropical countries. In summer, the disease is more serious and widely distributed and reported in various countries. The current study is aimed at identification of MYMV and molecular characterization of mung bean yellow mosaic virus in French bean. This study will be helpful for early detection of the virus, so that better preventive measures can be taken to control MYMV.Methods: The investigations were conducted during 2016-2017, all the agro climatic zones of Karnataka province were surveyed. The coat protein (CP) gene of MYMV was amplified using gene specific primer with DNA isolated from MYMV infected leaves samples in french bean. Polymerase chain reaction products were Sequenced and analysed using a bioinformatic tool. Result: The CP gene decrypt sequences analysis revealed that the identity and similarity in global alignment for all the obtained sequences ranging from 80.8 to 95.3% with reference to MYMV, whereas for horse gram yellow mosaic virus (HYMV) it ranges from 90.4 to 99.1%. Two distinct yellow mosaic viruses infecting french bean (MYMV and HYMV) were identified and it was observed that there exists considerable genetic variation among these species. Present work showed that the CP region is efficient enough to provide a simple, rapid and reliable method for early detection of MYMV infections in french bean, which would help to develop proper management strategies to control these viruses.

scholarly journals Single-Stranded DNA in Mung Bean Yellow Mosaic Virus

1985 ◽  
Vol 29 (8) ◽  
pp. 783-789 ◽  
Author(s):  
Masato Ikegami ◽  
Kazumori Yazaki ◽  
Yohachiro Honda ◽  
Mitsuro Iwaki ◽  
Hiroshi Fujii ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Mung Bean ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Single Stranded Dna

Serological Studies on Mung Bean Yellow Mosaic Virus

1988 ◽  
Vol 122 (2) ◽  
pp. 108-112 ◽  
Author(s):  
Masato Ikegami ◽  
Shin-ichi Shimizu
Keyword(s):  
Mosaic Virus ◽  
Mung Bean ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Serological Studies

Seed-borne nature of a begomovirus, Mung bean yellow mosaic virus in black gram

2015 ◽  
Vol 100 (4) ◽  
pp. 1925-1933 ◽  
Author(s):  
Satya Vijayalakshmi Kothandaraman ◽  
Alice Devadason ◽  
Malathi Varagur Ganesan
Keyword(s):  
Mosaic Virus ◽  
Mung Bean ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Black Gram
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